BBa_J92001
type: ComponentDefinition
persistent identity: BBa J92001
display id: BBa_J92001
version: 1
was generated by: iGEM to SBOL conversion
description: Lead Remover and Reporter Device
created: 2010-05-22T11:00:00Z
modified: 2015-05-08T01:08:30Z
mutable provenance: The majority of the subparts come from existing parts in the registry. We also designed a lead uptake part (Bba_J92000) which transports lead into the cell.
owned by: james
owned by: myers
mutable description: Our device has two main functions. First of all, it acts as an environmental lead sensor, alerting the user to the presence of lead 2+ ions. Secondly it bioaccumulates the lead ions and removes them from the solution.
discontinued: false
dominant: true
group u list: _415_
m user id: 0
owner id: 6514
owning group id: 415
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: In order to design this part we used the standard 23. We used the NEB biobrick assembly kit for this standard.
star: false
creator: Justin D Smith
type: DnaRegion
role: partType/Device
role: SO:0000804
component: BBa_J23119
component: BBa_I721002
component: BBa_B0034
component: BBa_J92000
component: BBa_I721001
component: BBa_B1006
component: BBa_I750016
component: BBa_K274100
sequence annotation: BBa_J23119
sequence annotation: BBa_B0034
sequence annotation: BBa_I721002
sequence annotation: BBa_J92000
sequence annotation: BBa_B1006
sequence annotation: BBa_I721001
sequence annotation: BBa_I750016
sequence annotation: BBa_K274100
sequence: https://synbiohub.org/public/igem/BBa_J92001_sequence/1

BBa_J23119
type: Component
persistent identity: component2236538
display id: component2236538
version: 1
top level: BBa_J92001
definition: BBa_J23119
access: public

BBa_I721002
type: Component
persistent identity: component2236541
display id: component2236541
version: 1
top level: BBa_J92001
definition: BBa_I721002
access: public

BBa_B0034
type: Component
persistent identity: component2236540
display id: component2236540
version: 1
top level: BBa_J92001
definition: BBa_B0034
access: public

BBa_J92000
type: Component
persistent identity: component2236544
display id: component2236544
version: 1
top level: BBa_J92001
definition: BBa_J92000
access: public

BBa_B0034
type: Component
persistent identity: component2236543
display id: component2236543
version: 1
top level: BBa_J92001
definition: BBa_B0034
access: public

BBa_I721001
type: Component
persistent identity: component2236550
display id: component2236550
version: 1
top level: BBa_J92001
definition: BBa_I721001
access: public

BBa_B1006
type: Component
persistent identity: component2236549
display id: component2236549
version: 1
top level: BBa_J92001
definition: BBa_B1006
access: public

BBa_B1006
type: Component
persistent identity: component2236581
display id: component2236581
version: 1
top level: BBa_J92001
definition: BBa_B1006
access: public

BBa_I750016
type: Component
persistent identity: component2236576
display id: component2236576
version: 1
top level: BBa_J92001
definition: BBa_I750016
access: public

BBa_K274100
type: Component
persistent identity: component2236597
display id: component2236597
version: 1
top level: BBa_J92001
definition: BBa_K274100
access: public

BBa_I721001
type: Component
persistent identity: component2236582
display id: component2236582
version: 1
top level: BBa_J92001
definition: BBa_I721001
access: public

BBa_B1006
type: Component
persistent identity: component2236602
display id: component2236602
version: 1
top level: BBa_J92001
definition: BBa_B1006
access: public

BBa_J23119
type: SequenceAnnotation
persistent identity: annotation2236538
display id: annotation2236538
version: 1
top level: BBa_J92001
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component: BBa_J23119

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persistent identity: range2236538
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top level: BBa_J92001
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start: 1
end: 35
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2236540
display id: annotation2236540
version: 1
top level: BBa_J92001
location: https://synbiohub.org/public/igem/BBa_J92001/annotation2236540/range2236540/1
component: BBa_B0034

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BBa_I721002
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version: 1
top level: BBa_J92001
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component: BBa_I721002

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BBa_B0034
type: SequenceAnnotation
persistent identity: annotation2236543
display id: annotation2236543
version: 1
top level: BBa_J92001
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component: BBa_B0034

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persistent identity: range2236543
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BBa_J92000
type: SequenceAnnotation
persistent identity: annotation2236544
display id: annotation2236544
version: 1
top level: BBa_J92001
location: https://synbiohub.org/public/igem/BBa_J92001/annotation2236544/range2236544/1
component: BBa_J92000

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BBa_B1006
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display id: annotation2236549
version: 1
top level: BBa_J92001
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component: BBa_B1006

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BBa_I721001
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display id: annotation2236550
version: 1
top level: BBa_J92001
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component: BBa_I721001

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BBa_I750016
type: SequenceAnnotation
persistent identity: annotation2236576
display id: annotation2236576
version: 1
top level: BBa_J92001
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component: BBa_I750016

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BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2236581
display id: annotation2236581
version: 1
top level: BBa_J92001
location: https://synbiohub.org/public/igem/BBa_J92001/annotation2236581/range2236581/1
component: BBa_B1006

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persistent identity: range2236581
display id: range2236581
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direction: direction/reverse
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end: 8701
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BBa_I721001
type: SequenceAnnotation
persistent identity: annotation2236582
display id: annotation2236582
version: 1
top level: BBa_J92001
location: https://synbiohub.org/public/igem/BBa_J92001/annotation2236582/range2236582/1
component: BBa_I721001

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persistent identity: range2236582
display id: range2236582
version: 1
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direction: direction/reverse
start: 8710
end: 8803
orientation: inline

BBa_K274100
type: SequenceAnnotation
persistent identity: annotation2236597
display id: annotation2236597
version: 1
top level: BBa_J92001
location: https://synbiohub.org/public/igem/BBa_J92001/annotation2236597/range2236597/1
component: BBa_K274100

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display id: range2236597
version: 1
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direction: direction/reverse
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end: 12196
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BBa_B1006
type: SequenceAnnotation
persistent identity: annotation2236602
display id: annotation2236602
version: 1
top level: BBa_J92001
location: https://synbiohub.org/public/igem/BBa_J92001/annotation2236602/range2236602/1
component: BBa_B1006

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type: Range
persistent identity: range2236602
display id: range2236602
version: 1
top level: BBa_J92001
direction: direction/reverse
start: 12205
end: 12243
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

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type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_I750016
type: ComponentDefinition
persistent identity: BBa I750016
display id: BBa_I750016
version: 1
was generated by: iGEM to SBOL conversion
description: Gas Vesicle polycistonic gene
created: 2007-10-20T11:00:00Z
modified: 2015-08-31T04:08:05Z
mutable provenance: This 5.7Kbp part contains 11 open reading frames coding for gas vesicle genes (gvpB,R,N,F,G,L,S,K,J,T and U) from Bacillus megaterium VT1660 AF053765. Original AF053765 DNA in pBluescriptIIKS plasmid (pNL29 in "Gas Vesicle genes identified in Bacillus megaterium and Functional Expression in Escherichia coli, Ning Li and Maura C. Cannon Jounal of bacteriology May 1998 p2450-2458).
owned by: james
owned by: myers
mutable description: This 5.7Kbp part contains 11 open reading frames coding for gas vesicle genes (gvpB,R,N,F,G,L,S,K,J,T and U) from Bacillus megaterium VT1660 AF053765. Promotion of the sequence results in expression of gas vesicles which slow the rate of settling of the cells in Saline.
discontinued: false
dominant: false
experience: experience/None
group u list: _132_
m user id: 0
owner id: 1721
owning group id: 9
sample status: It's complicated
status: status/Planning
bookmark: false
mutable notes: Site directed mutagenesis was performed in four rounds to remove 3 PstI sites and one EcoRI site from gvpL in the sequence. Primers with biobrick prefix and suffix were then used in a PCR reaction to amplify the part. Finally BBa_J61035 was used to provide the plasmid backbone which introduced a RBS at the front of the part.
star: true
creator: Phillip Dodson
type: DnaRegion
role: SO:0000804
role: partType/Generator
sequence annotation: b0034
sequence annotation: gvpB
sequence annotation: gvpR
sequence annotation: gvpN
sequence annotation: c->a
sequence annotation: gvpF
sequence annotation: gvpG
sequence annotation: gvpL
sequence annotation: t->c
sequence annotation: gvpL g213a
sequence annotation: gvpL g318a
sequence annotation: gvpL g351a
sequence annotation: t->a
sequence annotation: First copy of 40bp section
sequence annotation: gvpL g696a
sequence annotation: 10 repeats of 40bp induced durring mutagenesis
sequence annotation: gvpS
sequence annotation: gvpK
sequence annotation: gvpJ
sequence annotation: gvpT
sequence annotation: g->a
sequence annotation: gvpU
sequence: https://synbiohub.org/public/igem/BBa_I750016_sequence/1

b0034
type: SequenceAnnotation
persistent identity: annotation1951682
display id: annotation1951682
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951682/range1951682/1
role: SO:0000139
role: feature/rbs

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persistent identity: range1951682
display id: range1951682
version: 1
top level: BBa_I750016
direction: direction/forward
start: 1
end: 12
orientation: inline

gvpB
type: SequenceAnnotation
persistent identity: annotation1951683
display id: annotation1951683
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951683/range1951683/1
role: SO:0000316
role: feature/cds

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persistent identity: range1951683
display id: range1951683
version: 1
top level: BBa_I750016
direction: direction/forward
start: 19
end: 285
orientation: inline

gvpR
type: SequenceAnnotation
persistent identity: annotation1951684
display id: annotation1951684
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951684/range1951684/1
role: feature/cds
role: SO:0000316

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type: Range
persistent identity: range1951684
display id: range1951684
version: 1
top level: BBa_I750016
direction: direction/forward
start: 361
end: 627
orientation: inline

gvpN
type: SequenceAnnotation
persistent identity: annotation1951685
display id: annotation1951685
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951685/range1951685/1
role: feature/cds
role: SO:0000316

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type: Range
persistent identity: range1951685
display id: range1951685
version: 1
top level: BBa_I750016
direction: direction/forward
start: 656
end: 1583
orientation: inline

c->a
type: SequenceAnnotation
persistent identity: annotation1954599
display id: annotation1954599
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954599/range1954599/1
role: feature/s mutation
role: SO:1000008

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persistent identity: range1954599
display id: range1954599
version: 1
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end: 718
orientation: inline

gvpF
type: SequenceAnnotation
persistent identity: annotation1951686
display id: annotation1951686
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951686/range1951686/1
role: SO:0000316
role: feature/cds

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persistent identity: range1951686
display id: range1951686
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gvpG
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persistent identity: annotation1951687
display id: annotation1951687
version: 1
top level: BBa_I750016
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role: feature/cds
role: SO:0000316

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type: Range
persistent identity: range1951687
display id: range1951687
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2364
end: 2632
orientation: inline

gvpL
type: SequenceAnnotation
persistent identity: annotation1951688
display id: annotation1951688
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951688/range1951688/1
role: feature/cds
role: SO:0000316

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persistent identity: range1951688
display id: range1951688
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2633
end: 3842
orientation: inline

t->c
type: SequenceAnnotation
persistent identity: annotation1954600
display id: annotation1954600
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954600/range1954600/1
role: feature/s mutation
role: SO:1000008

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type: Range
persistent identity: range1954600
display id: range1954600
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2703
end: 2703
orientation: inline

t->c
type: SequenceAnnotation
persistent identity: annotation1954601
display id: annotation1954601
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954601/range1954601/1
role: feature/s mutation
role: SO:1000008

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persistent identity: range1954601
display id: range1954601
version: 1
top level: BBa_I750016
direction: direction/forward
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gvpL g213a
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persistent identity: annotation1951694
display id: annotation1951694
version: 1
top level: BBa_I750016
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role: SO:1000008
role: feature/s mutation

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persistent identity: range1951694
display id: range1951694
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2845
end: 2845
orientation: inline

gvpL g318a
type: SequenceAnnotation
persistent identity: annotation1951696
display id: annotation1951696
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951696/range1951696/1
role: SO:1000008
role: feature/s mutation

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persistent identity: range1951696
display id: range1951696
version: 1
top level: BBa_I750016
direction: direction/forward
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end: 2950
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gvpL g351a
type: SequenceAnnotation
persistent identity: annotation1951697
display id: annotation1951697
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951697/range1951697/1
role: feature/s mutation
role: SO:1000008

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type: Range
persistent identity: range1951697
display id: range1951697
version: 1
top level: BBa_I750016
direction: direction/forward
start: 2983
end: 2983
orientation: inline

t->a
type: SequenceAnnotation
persistent identity: annotation1954602
display id: annotation1954602
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954602/range1954602/1
role: SO:0001059
role: feature/mutation

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type: Range
persistent identity: range1954602
display id: range1954602
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3222
end: 3222
orientation: inline

First copy of 40bp section
type: SequenceAnnotation
persistent identity: annotation1954616
display id: annotation1954616
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954616/range1954616/1
role: feature/misc
role: SO:0000110

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type: Range
persistent identity: range1954616
display id: range1954616
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3304
end: 3343
orientation: inline

gvpL g696a
type: SequenceAnnotation
persistent identity: annotation1951698
display id: annotation1951698
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951698/range1951698/1
role: feature/s mutation
role: SO:1000008

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type: Range
persistent identity: range1951698
display id: range1951698
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3328
end: 3328
orientation: inline

10 repeats of 40bp induced durring mutagenesis
type: SequenceAnnotation
persistent identity: annotation1954598
display id: annotation1954598
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954598/range1954598/1
role: SO:0000110
role: feature/misc

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type: Range
persistent identity: range1954598
display id: range1954598
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3344
end: 3743
orientation: inline

gvpS
type: SequenceAnnotation
persistent identity: annotation1951689
display id: annotation1951689
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951689/range1951689/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951689/range1951689/1
type: Range
persistent identity: range1951689
display id: range1951689
version: 1
top level: BBa_I750016
direction: direction/forward
start: 3860
end: 4147
orientation: inline

gvpK
type: SequenceAnnotation
persistent identity: annotation1951690
display id: annotation1951690
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951690/range1951690/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951690/range1951690/1
type: Range
persistent identity: range1951690
display id: range1951690
version: 1
top level: BBa_I750016
direction: direction/forward
start: 4101
end: 4385
orientation: inline

gvpJ
type: SequenceAnnotation
persistent identity: annotation1951691
display id: annotation1951691
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951691/range1951691/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951691/range1951691/1
type: Range
persistent identity: range1951691
display id: range1951691
version: 1
top level: BBa_I750016
direction: direction/forward
start: 4407
end: 4709
orientation: inline

t->a
type: SequenceAnnotation
persistent identity: annotation1954603
display id: annotation1954603
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954603/range1954603/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1954603/range1954603/1
type: Range
persistent identity: range1954603
display id: range1954603
version: 1
top level: BBa_I750016
direction: direction/forward
start: 4532
end: 4532
orientation: inline

gvpT
type: SequenceAnnotation
persistent identity: annotation1951692
display id: annotation1951692
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951692/range1951692/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951692/range1951692/1
type: Range
persistent identity: range1951692
display id: range1951692
version: 1
top level: BBa_I750016
direction: direction/forward
start: 4746
end: 5624
orientation: inline

g->a
type: SequenceAnnotation
persistent identity: annotation1954604
display id: annotation1954604
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954604/range1954604/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1954604/range1954604/1
type: Range
persistent identity: range1954604
display id: range1954604
version: 1
top level: BBa_I750016
direction: direction/forward
start: 5070
end: 5070
orientation: inline

g->a
type: SequenceAnnotation
persistent identity: annotation1954605
display id: annotation1954605
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1954605/range1954605/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_I750016/annotation1954605/range1954605/1
type: Range
persistent identity: range1954605
display id: range1954605
version: 1
top level: BBa_I750016
direction: direction/forward
start: 5133
end: 5133
orientation: inline

gvpU
type: SequenceAnnotation
persistent identity: annotation1951693
display id: annotation1951693
version: 1
top level: BBa_I750016
location: https://synbiohub.org/public/igem/BBa_I750016/annotation1951693/range1951693/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_I750016/annotation1951693/range1951693/1
type: Range
persistent identity: range1951693
display id: range1951693
version: 1
top level: BBa_I750016
direction: direction/forward
start: 5648
end: 6064
orientation: inline

BBa_J23119
type: ComponentDefinition
persistent identity: BBa J23119
display id: BBa_J23119
version: 1
was generated by: iGEM to SBOL conversion
description: constitutive promoter family member
created: 2006-08-23T11:00:00Z
modified: 2015-08-31T04:08:40Z
mutable provenance: Overlap extension of synthetic oligonucleotides
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Later
discontinued: false
dominant: true
group u list: _52_
m user id: 0
owner id: 483
owning group id: 95
sample status: In stock
status: status/Available
bookmark: false
mutable notes: N/A
star: true
creator: John Anderson
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_J23119_sequence/1

BBa_I721001
type: ComponentDefinition
persistent identity: BBa I721001
display id: BBa_I721001
version: 1
was generated by: iGEM to SBOL conversion
description: Lead Promoter
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:53Z
mutable provenance: ralstonia metallidurans
owned by: james
owned by: myers
mutable description: Lead Promoter (Part 4) messed up EcoRI site
discontinued: false
dominant: false
experience: experience/None
group u list: _113_
m user id: 0
owner id: 1531
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: true
creator: Jeffrey Hofmann
type: DnaRegion
role: partType/Regulatory
role: SO:0000167
sequence: https://synbiohub.org/public/igem/BBa_I721001_sequence/1

BBa_I721002
type: ComponentDefinition
persistent identity: BBa I721002
display id: BBa_I721002
version: 1
was generated by: iGEM to SBOL conversion
description: Lead Binding Protein
created: 2007-10-25T11:00:00Z
modified: 2015-08-31T04:07:53Z
mutable provenance: ralstonia metallidurans
owned by: james
owned by: myers
mutable description: lead binding protein part 3
discontinued: false
dominant: false
experience: experience/None
group u list: _113_
m user id: 0
owner id: 1531
owning group id: 58
sample status: It's complicated
status: status/Planning
bookmark: true
mutable notes: none
star: true
creator: Jeffrey Hofmann
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I721002_sequence/1

BBa_B1006
type: ComponentDefinition
persistent identity: BBa B1006
display id: BBa_B1006
version: 1
was generated by: iGEM to SBOL conversion
description: Terminator (artificial, large, %T~>90)
created: 2006-08-30T11:00:00Z
modified: 2015-08-31T04:07:21Z
mutable provenance: modified E. coli thr terminator, replaced all A-T pairs in stem with C-G pairs
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Artificial terminator, estimated %T~>90% *8bp stem, 6nt loop *Bidirectional, estimated reverse %T~>90%
discontinued: false
dominant: true
experience: experience/Works
group u list: _41_
m user id: 0
owner id: 745
owning group id: 41
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Bidirectional, with the reverse estimated to be less effective than the forward. Has a polyA tail of 9 residues.
star: true
creator: Haiyao Huang
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: B1006
sequence annotation: PolyA
sequence annotation: modified thr terminator
sequence: https://synbiohub.org/public/igem/BBa_B1006_sequence/1

B1006
type: SequenceAnnotation
persistent identity: annotation1898428
display id: annotation1898428
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B1006/annotation1898428/range1898428/1
type: Range
persistent identity: range1898428
display id: range1898428
version: 1
top level: BBa_B1006
direction: direction/forward
start: 1
end: 39
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898431
display id: annotation1898431
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898431/range1898431/1
type: Range
persistent identity: range1898431
display id: range1898431
version: 1
top level: BBa_B1006
direction: direction/reverse
start: 1
end: 9
orientation: inline

modified thr terminator
type: SequenceAnnotation
persistent identity: annotation1898429
display id: annotation1898429
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B1006/annotation1898429/range1898429/1
type: Range
persistent identity: range1898429
display id: range1898429
version: 1
top level: BBa_B1006
direction: direction/forward
start: 10
end: 31
orientation: inline

PolyA
type: SequenceAnnotation
persistent identity: annotation1898430
display id: annotation1898430
version: 1
top level: BBa_B1006
location: https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
role: SO:0000553
role: feature/polya

https://synbiohub.org/public/igem/BBa_B1006/annotation1898430/range1898430/1
type: Range
persistent identity: range1898430
display id: range1898430
version: 1
top level: BBa_B1006
direction: direction/forward
start: 32
end: 39
orientation: inline

BBa_J92000
type: ComponentDefinition
persistent identity: BBa J92000
display id: BBa_J92000
version: 1
was generated by: iGEM to SBOL conversion
description: Lead Uptake Transporter
created: 2010-05-22T11:00:00Z
modified: 2015-05-08T01:08:30Z
mutable provenance: Borremans,B., Hobman,J.L., Provoost,A., Brown,N.L. and van Der Lelie,D. "Cloning and functional analysis of the pbr lead resistance determinant of Ralstonia metallidurans CH34" J. Bacteriol. 183 (19), 5651-5658 (2001)
owned by: james
owned by: myers
mutable description: This part was derived from the Pbr T lead uptake protein from Ralstonia metallidurans. The protein is responsible for the uptake of Pb 2+ ions. This part can be used for projects where one needs to uptake lead ions from the environment into the cell interior.
discontinued: false
dominant: false
group u list: _415_
m user id: 0
owner id: 6514
owning group id: 415
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: When designing the part we checked the gene sequence/restriction site profile to make sure the part would be compatible with standards 10 and 23.
star: false
creator: Justin D Smith
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_J92000_sequence/1

BBa_K274100
type: ComponentDefinition
persistent identity: BBa K274100
display id: BBa_K274100
version: 1
was generated by: iGEM to SBOL conversion
description: CrtEBI with rbs
created: 2009-09-17T11:00:00Z
modified: 2015-05-08T01:11:45Z
mutable provenance: Gene coding sequences come from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087), a type of Gram negative Enterobacteriaceae. This Composite Biobrick is created by standard assembly of Part BBa_K118014, Part BBa_K118006 and Part BBa_K118005.
owned by: james
owned by: myers
mutable description: This Composite Biobrick is created by standard assembly of Part BBa_K118014 (CrtE with rbs), Part BBa_K118006 (CrtB with rbs) and Part BBa_K118005 (CrtI with rbs), which are submitted by previous iGEM teams. The whole cassette is on plasmid pSB1A2 (high copy, Ampicillin resistance). Together, enzymes CrtE, CrtB and CrtI convert colourless farnesyl pyrophosphate to red lycopene (via intermediates geranylgeranyl pyroiphosphate and phytoene). The red lycopene pigment can then be used as a coloured signal output, e.g. for biosensors. There is already individual ribosome binding site before each enzyme gene sequence. Internal restriction sites have been removed by previous iGEM teams. Please refer to Parts BBa_K118014, BBa_K118006 and BBa_K118005 for more information on individual Biobricks components. This Composite Biobrick has been put under constitutive promoter R0011 (see Part BBa_K274110) and arabinose-inducible promoter I0500 (see Part BBa_274120), transformed and tested in E.coli strain MG1655. Amount of lycopene produced can be measured by photospectrometer with absorbance at 475nm (lycopene extraction using acetone).
discontinued: false
dominant: true
experience: experience/Works
group u list: _374_
m user id: 0
owner id: 5416
owning group id: 9
sample status: In stock
status: status/Available
bookmark: false
mutable notes: The colourless precursor, farnesyl pyrophosphate, is naturally produced in E.coli using Non-mevalonate Pathway (from pyruvate and glyceraldehyde-3-phosphate). Our experiences showed that the Composite Biobrick, when put under a promoter, works better in E.coli strains with up-regulated level of precursors. E.coli strain MG1655 demonostrates higher level of pigment (lycopene) production than strain TOP10.
star: false
creator: Siming Ma
type: DnaRegion
role: SO:0000804
role: partType/Composite
component: BBa_K118003
component: BBa_J15001
component: BBa_I742151
component: BBa_K118012
component: BBa_K118002
sequence annotation: BBa_K118012
sequence annotation: BBa_I742151
sequence annotation: BBa_J15001
sequence annotation: BBa_K118002
sequence annotation: BBa_K118003
sequence: https://synbiohub.org/public/igem/BBa_K274100_sequence/1

BBa_K118003
type: Component
persistent identity: component2021361
display id: component2021361
version: 1
top level: BBa_K274100
definition: BBa_K118003
access: public

BBa_J15001
type: Component
persistent identity: component2021357
display id: component2021357
version: 1
top level: BBa_K274100
definition: BBa_J15001
access: public

BBa_I742151
type: Component
persistent identity: component2021349
display id: component2021349
version: 1
top level: BBa_K274100
definition: BBa_I742151
access: public

BBa_K118012
type: Component
persistent identity: component2021348
display id: component2021348
version: 1
top level: BBa_K274100
definition: BBa_K118012
access: public

BBa_K118002
type: Component
persistent identity: component2021354
display id: component2021354
version: 1
top level: BBa_K274100
definition: BBa_K118002
access: public

BBa_J15001
type: Component
persistent identity: component2021352
display id: component2021352
version: 1
top level: BBa_K274100
definition: BBa_J15001
access: public

BBa_K118012
type: SequenceAnnotation
persistent identity: annotation2021348
display id: annotation2021348
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021348/range2021348/1
component: BBa_K118012

https://synbiohub.org/public/igem/BBa_K274100/annotation2021348/range2021348/1
type: Range
persistent identity: range2021348
display id: range2021348
version: 1
top level: BBa_K274100
direction: direction/reverse
start: 1
end: 4
orientation: inline

BBa_I742151
type: SequenceAnnotation
persistent identity: annotation2021349
display id: annotation2021349
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021349/range2021349/1
component: BBa_I742151

https://synbiohub.org/public/igem/BBa_K274100/annotation2021349/range2021349/1
type: Range
persistent identity: range2021349
display id: range2021349
version: 1
top level: BBa_K274100
direction: direction/reverse
start: 11
end: 922
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021352
display id: annotation2021352
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021352/range2021352/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274100/annotation2021352/range2021352/1
type: Range
persistent identity: range2021352
display id: range2021352
version: 1
top level: BBa_K274100
direction: direction/reverse
start: 931
end: 940
orientation: inline

BBa_K118002
type: SequenceAnnotation
persistent identity: annotation2021354
display id: annotation2021354
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021354/range2021354/1
component: BBa_K118002

https://synbiohub.org/public/igem/BBa_K274100/annotation2021354/range2021354/1
type: Range
persistent identity: range2021354
display id: range2021354
version: 1
top level: BBa_K274100
direction: direction/reverse
start: 947
end: 1879
orientation: inline

BBa_J15001
type: SequenceAnnotation
persistent identity: annotation2021357
display id: annotation2021357
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021357/range2021357/1
component: BBa_J15001

https://synbiohub.org/public/igem/BBa_K274100/annotation2021357/range2021357/1
type: Range
persistent identity: range2021357
display id: range2021357
version: 1
top level: BBa_K274100
direction: direction/reverse
start: 1888
end: 1897
orientation: inline

BBa_K118003
type: SequenceAnnotation
persistent identity: annotation2021361
display id: annotation2021361
version: 1
top level: BBa_K274100
location: https://synbiohub.org/public/igem/BBa_K274100/annotation2021361/range2021361/1
component: BBa_K118003

https://synbiohub.org/public/igem/BBa_K274100/annotation2021361/range2021361/1
type: Range
persistent identity: range2021361
display id: range2021361
version: 1
top level: BBa_K274100
direction: direction/reverse
start: 1904
end: 3385
orientation: inline

BBa_J15001
type: ComponentDefinition
persistent identity: BBa J15001
display id: BBa_J15001
version: 1
was generated by: iGEM to SBOL conversion
description: strong synthetic E. coli ribosome binding site with SacI site.
created: 2007-07-12T11:00:00Z
modified: 2015-08-31T04:08:32Z
mutable provenance: Synthetic.
owned by: james
owned by: myers
mutable description: This is a strong synthetic E. coli ribosome binding site. It is synthesised as two complementary oligonucleotides rather than being incorporated into a biobrick plasmid. It incorporates a SacI site overlapping the XbaI site, which is preserved when it is added to any other biobrick. This allows easy detection of the RBS after it has been added upstream of a biobrick coding sequence in a plasmid vector.
discontinued: false
dominant: false
experience: experience/None
group u list: _163_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: Note the presence of a SacI site overlapping the XbaI site, which is preserved when this biobrick is added to any other biobrick. At the time of writing, this biobrick is added as a short piece of DNA composed of two complementary oligonucleotides rather than being incorporated into a biobrick cloning vector by itself. It can be added upstream of a biobrick coding sequence, and its presence can easily be detected in miniprep DNA on a gel by using a SacI-SpeI or similar digest.
star: false
creator: Chris French
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: SacI
sequence annotation: rbs
sequence: https://synbiohub.org/public/igem/BBa_J15001_sequence/1

SacI
type: SequenceAnnotation
persistent identity: annotation1938045
display id: annotation1938045
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_J15001/annotation1938045/range1938045/1
type: Range
persistent identity: range1938045
display id: range1938045
version: 1
top level: BBa_J15001
direction: direction/forward
start: 1
end: 3
orientation: inline

rbs
type: SequenceAnnotation
persistent identity: annotation1938046
display id: annotation1938046
version: 1
top level: BBa_J15001
location: https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
role: SO:0000139
role: feature/rbs

https://synbiohub.org/public/igem/BBa_J15001/annotation1938046/range1938046/1
type: Range
persistent identity: range1938046
display id: range1938046
version: 1
top level: BBa_J15001
direction: direction/forward
start: 4
end: 10
orientation: inline

BBa_I742151
type: ComponentDefinition
persistent identity: BBa I742151
display id: BBa_I742151
version: 1
was generated by: iGEM to SBOL conversion
description: crtE (geranylgeranyl pyrophosphate synthase) coding sequence.
created: 2007-10-23T11:00:00Z
modified: 2015-08-31T04:08:03Z
mutable provenance: Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087.
owned by: james
owned by: myers
mutable description: crtE (geranylgeranyl pyrophosphate synthase) from Pantoea ananatis (formerly Erwinia uredovora) DSMZ 30080 (ATCC 19321). Accession: D90087. Part of the carotenoid biosynthesis parthway.
discontinued: false
dominant: false
group u list: _123_
m user id: 0
owner id: 837
owning group id: 163
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: No important considerations.
star: false
creator: Chris French
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence: https://synbiohub.org/public/igem/BBa_I742151_sequence/1

BBa_K118002
type: ComponentDefinition
persistent identity: BBa K118002
display id: BBa_K118002
version: 1
was generated by: iGEM to SBOL conversion
description: crtB coding sequence encoding phytoene synthase
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtB from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene synthase, part of the carotenoid biosynthesis pathway, which converts geranylgeranyl diphosphate to phytoene. Reference: Misawa,N., Nakagawa,M., Kobayashi,K., Yamano,S., Izawa,Y., Nakamura,K. and Harashima,K. Elucidation of the Erwinia uredovora carotenoid biosynthetic pathway by functional analysis of gene products expressed in Escherichia coli. J. Bacteriol. 172 (12), 6704-6712 (1990)
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: No special considerations.
star: true
creator: Andrew Hall
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: crtB coding sequence
sequence: https://synbiohub.org/public/igem/BBa_K118002_sequence/1

crtB coding sequence
type: SequenceAnnotation
persistent identity: annotation1995701
display id: annotation1995701
version: 1
top level: BBa_K118002
location: https://synbiohub.org/public/igem/BBa_K118002/annotation1995701/range1995701/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K118002/annotation1995701/range1995701/1
type: Range
persistent identity: range1995701
display id: range1995701
version: 1
top level: BBa_K118002
direction: direction/forward
start: 1
end: 930
orientation: inline

BBa_K118003
type: ComponentDefinition
persistent identity: BBa K118003
display id: BBa_K118003
version: 1
was generated by: iGEM to SBOL conversion
description: crtI coding sequence encoding phytoene dehydrogenase
created: 2008-07-23T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Pantoea ananatis genomic DNA
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: This is the coding sequence of crtI from Pantoea ananatis (formerly Erwinia uredovora) (Accession number D90087). It encodes phytoene dehydrogenase, part of the carotenoid biosynthesis pathway, which converts Phytoene to Lycopene.
discontinued: false
dominant: false
experience: experience/Works
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: In stock
status: status/Available
bookmark: true
mutable notes: The native crtI sequence contains two internal PstI sites. Primers crtImutf1 and crtImutf2 were ordered so that the MABEL protocol (invented for this purpose) could be used to remove these sites. The mutagenic primers were as follows: * crtImutf1: gtt tac agt aag gtt gcc * crtImutf2: agt aac tct ctg ttt gtg c In both cases, the PstI site CTGCAG was converted to CTGCAA, a silent mutation.
star: true
creator: Andrew Hall
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: crtI coding sequence
sequence annotation: PstI site removed
sequence: https://synbiohub.org/public/igem/BBa_K118003_sequence/1

crtI coding sequence
type: SequenceAnnotation
persistent identity: annotation1995702
display id: annotation1995702
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1
role: SO:0000316
role: feature/cds

https://synbiohub.org/public/igem/BBa_K118003/annotation1995702/range1995702/1
type: Range
persistent identity: range1995702
display id: range1995702
version: 1
top level: BBa_K118003
direction: direction/forward
start: 1
end: 1479
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968244
display id: annotation1968244
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K118003/annotation1968244/range1968244/1
type: Range
persistent identity: range1968244
display id: range1968244
version: 1
top level: BBa_K118003
direction: direction/forward
start: 478
end: 483
orientation: inline

PstI site removed
type: SequenceAnnotation
persistent identity: annotation1968245
display id: annotation1968245
version: 1
top level: BBa_K118003
location: https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1
role: SO:1000008
role: feature/s mutation

https://synbiohub.org/public/igem/BBa_K118003/annotation1968245/range1968245/1
type: Range
persistent identity: range1968245
display id: range1968245
version: 1
top level: BBa_K118003
direction: direction/forward
start: 889
end: 894
orientation: inline

BBa_K118012
type: ComponentDefinition
persistent identity: BBa K118012
display id: BBa_K118012
version: 1
was generated by: iGEM to SBOL conversion
description: Synthetic ribosome binding site added by Son of Babel procedure
created: 2008-08-18T11:00:00Z
modified: 2015-05-08T01:09:36Z
mutable provenance: Synthetic DNA
owned by: james
owned by: myers
mutable description: This is a 4bp synthetic ribosome binding site which can be added by fusion PCR using a reverse primer and the Son of Babel cloning system.
discontinued: false
dominant: false
experience: experience/None
group u list: _192_
m user id: 0
owner id: 3282
owning group id: 9
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: The part can be added by fusion PCR using a Son of Babel reverse primer.
star: false
creator: Andrew Hall
type: DnaRegion
role: SO:0000139
role: partType/RBS
sequence: https://synbiohub.org/public/igem/BBa_K118012_sequence/1

https://synbiohub.org/public/igem/BBa_J92001_sequence/1
type: Sequence
persistent identity: BBa J92001 sequence
display id: BBa_J92001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagctactagagaaagaggagaaatactagatgatgcggatcggtgaactgggcaagaaggcagattgcttggtgcagaccgtgcgcttttacgagtcagaaggcttgctgcccgagcctgcacgtagcgagggcaacttcaggctctatgacgaagtccatttgcagcgcttgctgttcatccgccgctgccgggcgaaggacatgacgctggatgagatccgtcaactgctgaacttacgggatcggccagagttgggctgcggcgaggtgaacgcgctggtcgacgctcatatcgcgcaagtgcggaccaagatgaaggaattgcgcgccttggagcgcgagttaatggatctgcgacgctcctgcgatagcgcccgaacctcgcgcgagtgcggcattctcaacagcttggccgagcccgcctgatactagagaaagaggagaaatactagatgcaagccctgcgtctcctttcgattgttctgctctctctgttcgtgacggtctcgaccgcacaggcagatcctctcgccacgcaagacaaggccaagcaaatctggcaagtactggactaccttgctgtcgactatggccggtccgtcaaggatggccacgtcgccaacgaagccgagtatgccgagatgcaggagttcgcgcaggccgctgaacggcagctaaccgagttgccgccgacgccagccgcgccagagttggccaaggaagcggcggcgttgcgcgcatccattgcagaaaaggccccgcccgaatcagtcggcgagcaagctcgcaaactggccggcggactcctcgcagcctatccggtcccgatggcccccggcaagctgcccgatctgcaacaaggggcaaagctgtatcaaagccaatgtgcttcgtgccacggcgtttccgggcacgcggacggcccactagctgcaaagctgagcccgccaccgatcgccctggctgatcacgagcgcgcccaggagcgcagcgtctttgccctacagcaaatcatcacgcgcggggtagaagggacgtcgatgccggcatttgctcaactctcggacgaggaacgctgggcgctcgcctattttgcctcgaccctttcgtattcggatgccgaccgccaggccggcgccaagctgtggacctctcaaccggctctccacgcagccgtgcccacgctggccttgttaagccagacctccgaggctgcgctcgccaagaccgtcggggcggatgccgctcgccaactgaccgcgtacctcaggagcacgccgggctcggtcaccgcgtcaagcaccgacagcctgatgatcgccagagacaagctgaaggaaagcttggccaccctggacaagggcgacaggcagttggcttcgcggctggcgttgtctgcctatctcgacggcttcgagcccgtcgagccggcgctggctgccaagaaccaggcgctgttccaggatatcgagaagacgatggggctctatcgcaatgctgtgacagcgggccaggtggaacgcgcccatgaaattgcgcagaagttgcaatcgcagcttgacgacgcacaggaggcgctgggcggcaccaatgacgccatatcgaccttcctcggcgccctgacaatcctgctacgagaagggcttgaagccctgttggtggtcgtcgcgatgatggcgttcctgaagaaagcggatcgcaccgatgtcctgccctacgtgcatgcaggctgggttactgcgctggcggccggtgggctgacctgggcggtagccacctatgtcgtcgacctgagtggcgccagtcgcgagatgacggaaggtttttccgcagtgtttgccgcaatcgtgttgcttggcgtgggcatgtggatgcaccagaagagtcttgcgggtcgctggcaggcctatgtgaaggcgaagctgtcctcggcattgaacaagaaatcagcgctgatgctgttcct…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I750016_sequence/1
type: Sequence
persistent identity: BBa I750016 sequence
display id: BBa_I750016_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgtctattcaaaaaagtactaatagttcaagtttagcagaagtcattgaccgtattttagataaaggaattgttattgatgcttttgcaagagtttctgttgtaggaattgaaattttaacgattgaagcgcgagtggttattgccagtgttgatacatggttacgctatgcagaagcagtagggcttcttcgtgacgacgtagaagaaaacggtcttcctgaacgttcaaattcaagtgaagggcagccgcgttttagtatttaatatgaagtggtatggaaccttccttttttgagggtggttccagcttttagatctaactattggaggctactaaaaatggaaattaaaaaaattatgcaagccgtgaacgactttttcggtgaacacgtagctcctcctcataaaattacctcggtggaagctactgaagatgaaggttggagagttattgttgaagtcattgaagaacgagaatatatgaaaaaatacgccaaagatgaaatgctcggaacgtacgagtgctttgtaaataaagaaaaagaagtcatttcattcaaacgactcgacgtcagatatagaagcgccattggcattgaagcataaacgataagatggcaggaggaacgtaaaaatgaccgtcttaacagacaaaaggaaaaaaggcagtggagcttttatacaagatgacgagacaaaagaggttctttcaagagcgctgagctatttaaaatccggctattccattcattttacaggtcctgccggcggaggcaaaacctctttagcgcgagcgcttgctaaaaagagaaagcgtcctgtaatgctgatgcacgggaatcacgagctcaacaacaaagatttaattggcgattttacgggatacacgagcaaaaaagtaatcgaccagtacgttcgttctgtctataaaaaagatgaacaggtgagtgaaaactggcaggatggccgattgcttgaagctgtaaaaaatggctatacgctgatttacgacgaatttactcgttctaagcctgcgacgaataatatctttctatcgatattagaagaaggcgtgctgccgctgtatggagtaaaaatgaccgatccttttgtgcgcgtgcatcccgatttccgcgtcatcttcacaagcaatccagctgagtatgccggcgtatatgatacgcaagatgcgcttctcgacaggttaattaccatgtttattgattataaagacatcgacagagagacagcgattttaacggagaaaacggacgtagaagaagatgaagcgcgcacaattgtaacgctcgtagcaaacgtgcgaaaccgctctggagacgaaaacagcagcggacttagcctgcgggcttcgcttatgatcgctacccttgccacgcagcaagacattcctatcgatggaagtgacgaagattttcaaacgttatgtatcgatattttgcatcatccgcttaccaaatgtttggatgaagaaaatgcaaaaagcaaagccgaaaaaatcattttagaagaatgtaagaatatagacactgaagaaaagtaaaggagcttgaaaacatgagtgaaacaaacgaaacaggtatttatatttttagcgccattcaaacggataaagacgaagaatttggcgccgtggaagtagaaggaacaaaagctgaaacatttttgattcgctacaaagacgcggctatggtagcagctgaagtaccgatgaaaatttatcatcctaatcgccaaaatttattaatgcatcaaaacgcagtagcagcgattatggacaagaacgatacggttattccaatcagctttgggaatgtattcaaatcaaaagaagacgtaaaagttcttttggaaaacctttatccgcagtttgaaaagctgtttccagcgatcaaaggaaaaattgaagtcggtttaaaagtaattgggaaaaaagaatggcttgagaaaaaagtaaacga…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J23119_sequence/1
type: Sequence
persistent identity: BBa J23119 sequence
display id: BBa_J23119_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ttgacagctagctcagtcctaggtataatgctagc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I721001_sequence/1
type: Sequence
persistent identity: BBa I721001 sequence
display id: BBa_I721001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ggttgcttcctataaaaaacttgactctatatctactagaggttttctaatgatggcatccggggaaaaccttgtcaatgaagagcgatctatg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I721002_sequence/1
type: Sequence
persistent identity: BBa I721002 sequence
display id: BBa_I721002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgatgcggatcggtgaactgggcaagaaggcagattgcttggtgcagaccgtgcgcttttacgagtcagaaggcttgctgcccgagcctgcacgtagcgagggcaacttcaggctctatgacgaagtccatttgcagcgcttgctgttcatccgccgctgccgggcgaaggacatgacgctggatgagatccgtcaactgctgaacttacgggatcggccagagttgggctgcggcgaggtgaacgcgctggtcgacgctcatatcgcgcaagtgcggaccaagatgaaggaattgcgcgccttggagcgcgagttaatggatctgcgacgctcctgcgatagcgcccgaacctcgcgcgagtgcggcattctcaacagcttggccgagcccgcctga
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B1006_sequence/1
type: Sequence
persistent identity: BBa B1006 sequence
display id: BBa_B1006_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaaaaaaaaccccgcccctgacagggcggggtttttttt
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J92000_sequence/1
type: Sequence
persistent identity: BBa J92000 sequence
display id: BBa_J92000_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgcaagccctgcgtctcctttcgattgttctgctctctctgttcgtgacggtctcgaccgcacaggcagatcctctcgccacgcaagacaaggccaagcaaatctggcaagtactggactaccttgctgtcgactatggccggtccgtcaaggatggccacgtcgccaacgaagccgagtatgccgagatgcaggagttcgcgcaggccgctgaacggcagctaaccgagttgccgccgacgccagccgcgccagagttggccaaggaagcggcggcgttgcgcgcatccattgcagaaaaggccccgcccgaatcagtcggcgagcaagctcgcaaactggccggcggactcctcgcagcctatccggtcccgatggcccccggcaagctgcccgatctgcaacaaggggcaaagctgtatcaaagccaatgtgcttcgtgccacggcgtttccgggcacgcggacggcccactagctgcaaagctgagcccgccaccgatcgccctggctgatcacgagcgcgcccaggagcgcagcgtctttgccctacagcaaatcatcacgcgcggggtagaagggacgtcgatgccggcatttgctcaactctcggacgaggaacgctgggcgctcgcctattttgcctcgaccctttcgtattcggatgccgaccgccaggccggcgccaagctgtggacctctcaaccggctctccacgcagccgtgcccacgctggccttgttaagccagacctccgaggctgcgctcgccaagaccgtcggggcggatgccgctcgccaactgaccgcgtacctcaggagcacgccgggctcggtcaccgcgtcaagcaccgacagcctgatgatcgccagagacaagctgaaggaaagcttggccaccctggacaagggcgacaggcagttggcttcgcggctggcgttgtctgcctatctcgacggcttcgagcccgtcgagccggcgctggctgccaagaaccaggcgctgttccaggatatcgagaagacgatggggctctatcgcaatgctgtgacagcgggccaggtggaacgcgcccatgaaattgcgcagaagttgcaatcgcagcttgacgacgcacaggaggcgctgggcggcaccaatgacgccatatcgaccttcctcggcgccctgacaatcctgctacgagaagggcttgaagccctgttggtggtcgtcgcgatgatggcgttcctgaagaaagcggatcgcaccgatgtcctgccctacgtgcatgcaggctgggttactgcgctggcggccggtgggctgacctgggcggtagccacctatgtcgtcgacctgagtggcgccagtcgcgagatgacggaaggtttttccgcagtgtttgccgcaatcgtgttgcttggcgtgggcatgtggatgcaccagaagagtcttgcgggtcgctggcaggcctatgtgaaggcgaagctgtcctcggcattgaacaagaaatcagcgctgatgctgttcctgctgtcgttcgtgaccgtctatcgagaagtgtttgaaacagtcctcttttacgctgccctgtggacagagggcaacggtgtatttttgctcgcaggacttgcgtcgggtatcgccatcctggccgcgatcgccatcgtgctcctgcgctccacggcgcgcctgccgattggtcagttctttgccttcagctcagcattggtcggcgtgttggcggtcgttctgattggtaaaggcatagctgcgcttcagaaagtcggcttcctacaagttacgccgatctccatgcctcgtatcgacgtcctgggcatttatccgtccgtccagaccgtcgtagcgcaagtcctgattctgctcattatcgccgcaagcgtcgtctacaatttgcggtcgcaccggccatctacccaggtgcaccaccaccaccaccactaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K274100_sequence/1
type: Sequence
persistent identity: BBa K274100 sequence
display id: BBa_K274100_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gaggtactagatgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataatactagagctcaaggaggtactagatgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataatactagagctcaaggaggtactagatgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaac…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_J15001_sequence/1
type: Sequence
persistent identity: BBa J15001 sequence
display id: BBa_J15001_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ctcaaggagg
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_I742151_sequence/1
type: Sequence
persistent identity: BBa I742151 sequence
display id: BBa_I742151_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgacggtctgcgcaaaaaaacacgttcatctcactcgcgatgctgcggagcagttactggctgatattgatcgacgccttgatcagttattgcccgtggagggagaacgggatgttgtgggtgccgcgatgcgtgaaggtgcgctggcaccgggaaaacgtattcgccccatgttgctgttgctgaccgcccgcgatctgggttgcgctgtcagccatgacggattactggatttggcctgtgcggtggaaatggtccacgcggcttcgctgatccttgacgatatgccctgcatggacgatgcgaagctgcggcgcggacgccctaccattcattctcattacggagagcatgtggcaatactggcggcggttgccttgctgagtaaagcctttggcgtaattgccgatgcagatggcctcacgccgctggcaaaaaatcgggcggtttctgaactgtcaaacgccatcggcatgcaaggattggttcagggtcagttcaaggatctgtctgaaggggataagccgcgcagcgctgaagctattttgatgacgaatcactttaaaaccagcacgctgttttgtgcctccatgcagatggcctcgattgttgcgaatgcctccagcgaagcgcgtgattgcctgcatcgtttttcacttgatcttggtcaggcatttcaactgctggacgatttgaccgatggcatgaccgacaccggtaaggatagcaatcaggacgccggtaaatcgacgctggtcaatctgttaggcccgagggcggttgaagaacgtctgagacaacatcttcagcttgccagtgagcatctctctgcggcctgccaacacgggcacgccactcaacattttattcaggcctggtttgacaaaaaactcgctgccgtcagttaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118002_sequence/1
type: Sequence
persistent identity: BBa K118002 sequence
display id: BBa_K118002_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaataatccgtcgttactcaatcatgcggtcgaaacgatggcagttggctcgaaaagttttgcgacagcctcaaagttatttgatgcaaaaacccggcgcagcgtactgatgctctacgcctggtgccgccattgtgacgatgttattgacgatcagacgctgggctttcaggcccggcagcctgccttacaaacgcccgaacaacgtctgatgcaacttgagatgaaaacgcgccaggcctatgcaggatcgcagatgcacgaaccggcgtttgcggcttttcaggaagtggctatggctcatgatatcgccccggcttacgcgtttgatcatctggaaggcttcgccatggatgtacgcgaagcgcaatacagccaactggatgatacgctgcgctattgctatcacgttgcaggcgttgtcggcttgatgatggcgcaaatcatgggcgtgcgggataacgccacgctggaccgcgcctgtgaccttgggctggcatttcagttgaccaatattgctcgcgatattgtggacgatgcgcatgcgggccgctgttatctgccggcaagctggctggagcatgaaggtctgaacaaagagaattatgcggcacctgaaaaccgtcaggcgctgagccgtatcgcccgtcgtttggtgcaggaagcagaaccttactatttgtctgccacagccggcctggcagggttgcccctgcgttccgcctgggcaatcgctacggcgaagcaggtttaccggaaaataggtgtcaaagttgaacaggccggtcagcaagcctgggatcagcggcagtcaacgaccacgcccgaaaaattaacgctgctgctggccgcctctggtcaggcccttacttcccggatgcgggctcatcctccccgccctgcgcatctctggcagcgcccgctctaataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118003_sequence/1
type: Sequence
persistent identity: BBa K118003 sequence
display id: BBa_K118003_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaccaactacggtaattggtgcaggcttcggtggcctggcactggcaattcgtctacaagctgcggggatccccgtcttactgcttgaacaacgtgataaacccggcggtcgggcttatgtctacgaggatcaggggtttacctttgatgcaggcccgacggttatcaccgatcccagtgccattgaagaactgtttgcactggcaggaaaacagttaaaagagtatgtcgaactgctgccggttacgccgttttaccgcctgtgttgggagtcagggaaggtctttaattacgataacgatcaaacccggctcgaagcgcagattcagcagtttaatccccgcgatgtcgaaggttatcgtcagtttctggactattcacgcgcggtgtttaaagaaggctatctaaagctcggtactgtcccttttttatcgttcagagacatgcttcgcgccgcacctcaactggcgaaactgcaagcatggagaagcgtttacagtaaggttgccagttacatcgaagatgaacatctgcgccaggcgttttctttccactcgctgttggtgggcggcaatcccttcgccacctcatccatttatacgttgatacacgcgctggagcgtgagtggggcgtctggtttccgcgtggcggcaccggcgcattagttcaggggatgataaagctgtttcaggatctgggtggcgaagtcgtgttaaacgccagagtcagccatatggaaacgacaggaaacaagattgaagccgtgcatttagaggacggtcgcaggttcctgacgcaagccgtcgcgtcaaatgcagatgtggttcatacctatcgcgacctgttaagccagcaccctgccgcggttaagcagtccaacaaactgcaaactaagcgcatgagtaactctctgtttgtgctctattttggtttgaatcaccatcatgatcagctcgcgcatcacacggtttgtttcggcccgcgttaccgcgagctgattgacgaaatttttaatcatgatggcctcgcagaggacttctcactttatctgcacgcgccctgtgtcacggattcgtcactggcgcctgaaggttgcggcagttactatgtgttggcgccggtgccgcatttaggcaccgcgaacctcgactggacggttgaggggccaaaactacgcgaccgtatttttgcgtaccttgagcagcattacatgcctggcttacggagtcagctggtcacgcaccggatgtttacgccgtttgattttcgcgaccagcttaatgcctatcatggctcagccttttctgtggagcccgttcttacccagagcgcctggtttcggccgcataaccgcgataaaaccattactaatctctacctggtcggcgcaggcacgcatcccggcgcaggcattcctggcgtcatcggctcggcaaaagcgacagcaggtttgatgctggaggatctgatataataa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_K118012_sequence/1
type: Sequence
persistent identity: BBa K118012 sequence
display id: BBa_K118012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: gagg
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z