##gff-version 3
##sequence-region BBa_J92210 1 4343
BBa_J92210	.	mature_transcript_region	1	136	.	+	0	ID=BBa_K228001;Name=BBa_K228001
BBa_J92210	.	engineered_region	626	1527	.	+	0	ID=BBa_K173005;Name=BBa_K173005
BBa_J92210	.	promoter	1474	1527	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_K173005
BBa_J92210	.	promoter	1516	1521	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_J92210	.	non_covalent_binding_site	1474	1492	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_J92210	.	promoter	1493	1498	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_J92210	.	non_covalent_binding_site	1499	1517	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_J92210	.	engineered_region	1474	1527	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_J92210	.	engineered_region	626	1465	.	+	0	ID=BBa_P0440;Name=BBa_P0440;Parent=BBa_K173005
BBa_J92210	.	terminator	1337	1416	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0440
BBa_J92210	.	stem_loop	1348	1391	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J92210	.	engineered_region	1337	1416	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J92210	.	ribosome_entry_site	626	637	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0440
BBa_J92210	.	conserved_region	630	633	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_J92210	.	terminator	1425	1465	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0440
BBa_J92210	.	polyA_site	1452	1465	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J92210	.	stem_loop	1432	1451	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J92210	.	engineered_region	1425	1465	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J92210	.	stop_codon	1458	1458	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J92210	.	CDS	644	1303	.	+	0	ID=BBa_C0040;Name=BBa_C0040;Parent=BBa_P0440
BBa_J92210	.	engineered_tag	1304	1328	.	+	0	ID=annotation2213989;Name=Help:Barcodes;Parent=BBa_C0040
BBa_J92210	.	CDS	647	1263	.	+	0	ID=annotation23329;Name=tetR;Parent=BBa_C0040
BBa_J92210	.	tag	1264	1297	.	+	0	ID=annotation23330;Name=SsrA;Parent=BBa_C0040
BBa_J92210	.	terminator	4303	4343	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_J92210	.	polyA_site	4330	4343	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J92210	.	stem_loop	4310	4329	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J92210	.	engineered_region	4303	4343	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J92210	.	stop_codon	4336	4336	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J92210	.	engineered_region	145	617	.	+	0	ID=BBa_K274370;Name=BBa_K274370
BBa_J92210	.	engineered_region	145	381	.	+	0	ID=BBa_I746350;Name=BBa_I746350;Parent=BBa_K274370
BBa_J92210	.	engineered_region	145	156	.	+	0	ID=annotation1943865;Name=B0034;Parent=BBa_I746350
BBa_J92210	.	start_codon	163	163	.	+	0	ID=annotation1943866;Name=P2 ogr;Parent=BBa_I746350
BBa_J92210	.	CDS	163	381	.	+	0	ID=annotation1943867;Name=P2 ogr;Parent=BBa_I746350
BBa_J92210	.	promoter	527	617	.	+	0	ID=BBa_I746360;Name=BBa_I746360;Parent=BBa_K274370
BBa_J92210	.	promoter	527	617	.	+	0	ID=annotation1943783;Name=PF;Parent=BBa_I746360
BBa_J92210	.	terminator	390	469	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_K274370
BBa_J92210	.	stem_loop	401	444	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J92210	.	engineered_region	390	469	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J92210	.	terminator	478	518	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_K274370
BBa_J92210	.	polyA_site	505	518	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_J92210	.	stem_loop	485	504	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_J92210	.	engineered_region	478	518	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_J92210	.	stop_codon	511	511	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_J92210	.	terminator	4215	4294	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_J92210	.	stem_loop	4226	4269	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_J92210	.	engineered_region	4215	4294	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_J92210	.	CDS	1534	4206	.	+	0	ID=BBa_K228000;Name=BBa_K228000
BBa_J92210	.	CDS	1534	4206	.	+	0	ID=annotation2017714;Name=cds;Parent=BBa_K228000
>BBa_J92210
caattcggagagatgccggagcggctgaacggaccggtctctaaaaccggagtaggggcaactctaccgggggttcaaa
tccccctctctccgccactacagatccttagcgaaagctaaggattttttttaagcttactagagaaagaggagaaata
ctagatgtttcattgtcctttatgccagcatgccgcacatgcgcgtacaagtcgctatatcactgacacgacaaaagag
cgttatcatcagtgccagaacgtgaattgcagcgccacgttcatcacttatgagtcggtacagcgatacatcgtgaagc
cgggagaagtccacgccgtaaggccgcacccgttgccatcagggcagcaaattatgtggatgtaatactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagttgcccgccttttctttaccggtggtt
gtgctgtcgattagccaaccgggacaaatagcctgacatctccggcgcaactgaaaataccacttactagagaaagagg
agaaatactagatgtccagattagataaaagtaaagtgattaacagcgcattagagctgcttaatgaggtcggaatcga
aggtttaacaacccgtaaactcgcccagaagctaggtgtagagcagcctacattgtattggcatgtaaaaaataagcgg
gctttgctcgacgccttagccattgagatgttagataggcaccatactcacttttgccctttagaaggggaaagctggc
aagattttttacgtaataacgctaaaagttttagatgtgctttactaagtcatcgcgatggagcaaaagtacatttagg
tacacggcctacagaaaaacagtatgaaactctcgaaaatcaattagcctttttatgccaacaaggtttttcactagag
aatgcattatatgcactcagcgctgtggggcattttactttaggttgcgtattggaagatcaagagcatcaagtcgcta
aagaagaaagggaaacacctactactgatagtatgccgccattattacgacaagctatcgaattatttgatcaccaagg
tgcagagccagccttcttattcggccttgaattgatcatatgcggattagaaaaacaacttaaatgtgaaagtgggtcc
gctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagccaggca
tcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactag
agtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatcc
ctatcagtgatagagatactgagcactactagatgaccatgattaccgtgcactagaataccattaacattgctaagaa
cgacttctctgacatcgaactggctgctatcccgttcaacactctggctgaccattacggtgagcgtttagctcgcgaa
cagttggcccttgagcatgagtcttacgagatgggtgaagcacgcttccgcaagatgtttgagcgtcaacttaaagctg
gtgaggttgcggataacgctgccgccaagcctctcatcactaccctactccctaagatgattgcacgcatcaacgactg
gtttgaggaagtgaaagctaagcgcggcaagcgcccgacagccttccagttcctgtaggaaatcaagccggaagccgta
gcgtacatcaccattaagaccactctggcttgcctaaccagtgctgacaatacaaccgttcaggctgtagcaagcgcaa
tcggtcgggccattgaggacgaggctcgcttcggtcgtatccgtgaccttgaagctaagcacttcaagaaaaacgttga
ggaacaactcaacaagcgcgtagggcacgtctacaagaaagcatttatgcaagttgtcgaggctgacatgctctctaag
ggtctactcggtggcgaggcgtggtcttcgtggcataaggaagactctattcatgtaggagtacgctgcatcgagatgc
tcattgagtcaaccggaatggttagcttacaccgccaaaatgctggcgtagtaggtcaagactctgagactatcgaact
cgcacctgaatacgctgaggctatcgcaacccgtgcaggtgcgctggctggcatctctccgatgttccaaccttgcgta
gttcctcctaagccgtggactggcattactggtggtggctattgggctaacggtcgtcgtcctctggcgctggtgcgta
ctcacagtaagaaagcactgatgcgctacgaagacgtttacatgcctgaggtgtacaaagcgattaacattgcgcaaaa
caccgcatggaaaatcaacaagaaagtcctagcggtcgccaacgtaatcaccaagtggaagcattgtccggtcgaggac
atccctgcgattgagcgtgaagaactcccgatgaaaccggaagacatcgacatgaatcctgaggctctcaccgcgtgga
aacgtgctgccgctgctgtgtaccgcaaggacaaggctcgcaagtctcgccgtatcagccttgagttcatgcttgagca
agccaataagtttgctaaccataaggccatctggttcccttacaacatggactggcgcggtcgtgtttacgctgtgtca
atgttcaacccgcaaggtaacgatatgaccaaaggactgcttacgctggcgaaaggtaaaccaatcggtaaggaaggtt
actactggctgaaaatccacggtgcaaactgtgcgggtgtcgataaggttccgttccctgagcgcatcaagttcattga
ggaaaaccacgagaacatcatggcttgcgctaagtctccactggagaacacttggtgggctgagcaagattctccgttc
tgcttccttgcgttctgctttgagtacgctggggtacagcaccacggcctgagctataactgctcccttccgctggcgt
ttgacgggtcttgctctggcatccagcacttctccgcgatgctccgagatgaggtaggtggtcgcgcggttaacttgct
tcctagtgaaaccgttcaggacatctacgggattgttgctaagaaagtcaacgagattctacaagcagacgcaatcaat
gggaccgataacgaagtagttaccgtgaccgatgagaacactggtgaaatctctgagaaagtcaagctgggcactaagg
cactggctggtcaatggctggcttacggtgttactcgcagtgtgactaagcgttcagtcatgacgctggcttacgggtc
caaagagttcggcttccgtcaacaagtgctggaagataccattcagccagctattgattccggcaagggtctgatgttc
actcagccgaatcaggctgctggatacatggctaagctgatttgggaatctgtgagcgtgacggtggtagctgcggttg
aagcaatgaactggcttaagtctgctgctaagctgctggctgctgaggtcaaagataagaagactggagagattcttcg
caagcgttgcgctgtgcattgggtaactcctgatggtttccctgtgtggcaggaatacaagaagcctattcagacgcgc
ttgaacctgatgttcctcggtcagttccgcttacagcctaccattaacaccaacaaagatagcgagattgatgcacaca
aacaggagtctggtatcgctcctaactttgtacacagccaagacggtagccaccttcgtaagactgtagtgtgggcaca
cgagaagtacggaatcgaatcttttgcactgattcacgactccttcggtaccattccggctgacgctgcgaacctgttc
aaagcagtgcgcgaaactatggttgacacatatgagtcttgtgatgtactggctgatttctacgaccagttcgctgacc
agttgcacgagtctcaattggacaaaatgccagcacttccggctaaaggtaacttgaacctccgtgacatcttagagtc
ggacttcgcgttcgcataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttt
tatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata