LOCUS       BBa_J95012              2927 bp    DNA     linear       22-Dec-2024
DEFINITION  Reference standard for BioBrick promoter and RBS characterization
ACCESSION   BBa_J95012
VERSION     BBa_J95012.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     misc_feature    63..1715
                     /label=BBa_J95000
     stem_loop       1731..1750
                     /label=T7 TE
     misc_feature    1757..1757
                     /label=stop
     polyA_site      1751..1764
                     /label=polya
     misc_feature    1724..1764
                     /label=BBa_B0012
     terminator      1724..1764
                     /label=BBa_B0012
     stem_loop       1785..1807
                     /label=stem_loop
     misc_feature    1773..1818
                     /label=BBa_B0011
     terminator      1773..1818
                     /label=BBa_B0011
     promoter        1827..1861
                     /label=BBa_J23101
     RBS             1870..1882
                     /label=RBS-3\Weak
     RBS             1876..1879
                     /label=RBS
     misc_feature    1870..1882
                     /label=BBa_B0032
     RBS             1870..1882
                     /label=BBa_B0032
     misc_feature    1889..2824
                     /label=BBa_J95001
     stem_loop       2840..2859
                     /label=T7 TE
     misc_feature    2866..2866
                     /label=stop
     polyA_site      2860..2873
                     /label=polya
     misc_feature    2833..2873
                     /label=BBa_B0012
     terminator      2833..2873
                     /label=BBa_B0012
     stem_loop       2894..2916
                     /label=stem_loop
     misc_feature    2882..2927
                     /label=BBa_B0011
     terminator      2882..2927
                     /label=BBa_B0011
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg
      121 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
      181 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt
      241 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
      301 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
      361 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca
      421 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
      481 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg
      541 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
      601 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg
      661 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct
      721 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
      781 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
      841 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt
      901 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc
      961 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct
     1021 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat
     1081 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
     1141 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
     1201 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg
     1261 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
     1321 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt
     1381 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc
     1441 aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac
     1501 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
     1561 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
     1621 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
     1681 taaaggccaa gaagggcgga aagatcgccg tgtaatacta gagtcacact ggctcacctt
     1741 cgggtgggcc tttctgcgtt tatatactag agagagaata taaaaagcca gattattaat
     1801 ccggcttttt tattatttta ctagagttta cagctagctc agtcctaggt attatgctag
     1861 ctactagagt cacacaggaa agtactagat ggcttccaag gtgtacgacc ccgagcaacg
     1921 caaacgcatg atcactgggc ctcagtggtg ggctcgctgc aagcaaatga acgtgctgga
     1981 ctccttcatc aactactatg attccgagaa gcacgccgag aacgccgtga tttttctgca
     2041 tggtaacgct gcctccagct acctgtggag gcacgtcgtg cctcacatcg agcccgtggc
     2101 tagatgcatc atccctgatc tgatcggaat gggtaagtcc ggcaagagcg ggaatggctc
     2161 atatcgcctc ctggatcact acaagtacct caccgcttgg ttcgagctgc tgaaccttcc
     2221 aaagaaaatc atctttgtgg gccacgactg gggggcttgt ctggcctttc actactccta
     2281 cgagcaccaa gacaagatca aggccatcgt ccatgctgag agtgtcgtgg acgtgatcga
     2341 gtcctgggac gagtggcctg acatcgagga ggatatcgcc ctgatcaaga gcgaagaggg
     2401 cgagaaaatg gtgcttgaga ataacttctt cgtcgagacc atgctcccaa gcaagatcat
     2461 gcggaaactg gagcctgagg agttcgctgc ctacctggag ccattcaagg agaagggcga
     2521 ggttagacgg cctaccctct cctggcctcg cgagatccct ctcgttaagg gaggcaagcc
     2581 cgacgtcgtc cagattgtcc gcaactacaa cgcctacctt cgggccagcg acgatctgcc
     2641 taagatgttc atcgagtccg accctgggtt cttttccaac gctattgtcg agggagctaa
     2701 gaagttccct aacaccgagt tcgtgaaggt gaagggcctc cacttcagcc aggaggacgc
     2761 tccagatgaa atgggtaagt acatcaagag cttcgtggag cgcgtgctga agaacgagca
     2821 gtaatactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga
     2881 gagagaatat aaaaagccag attattaatc cggctttttt attattt
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