LOCUS       BBa_J95013              2927 bp    DNA     linear       22-Dec-2024
DEFINITION  Reference standard for BioBrick promoter and RBS characterization
ACCESSION   BBa_J95013
VERSION     BBa_J95013.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     misc_feature    63..998
                     /label=BBa_J95001
     stem_loop       1014..1033
                     /label=T7 TE
     misc_feature    1040..1040
                     /label=stop
     polyA_site      1034..1047
                     /label=polya
     misc_feature    1007..1047
                     /label=BBa_B0012
     terminator      1007..1047
                     /label=BBa_B0012
     stem_loop       1068..1090
                     /label=stem_loop
     misc_feature    1056..1101
                     /label=BBa_B0011
     terminator      1056..1101
                     /label=BBa_B0011
     promoter        1110..1144
                     /label=BBa_J23101
     RBS             1153..1165
                     /label=RBS-3\Weak
     RBS             1159..1162
                     /label=RBS
     misc_feature    1153..1165
                     /label=BBa_B0032
     RBS             1153..1165
                     /label=BBa_B0032
     misc_feature    1172..2824
                     /label=BBa_J95000
     stem_loop       2840..2859
                     /label=T7 TE
     misc_feature    2866..2866
                     /label=stop
     polyA_site      2860..2873
                     /label=polya
     misc_feature    2833..2873
                     /label=BBa_B0012
     terminator      2833..2873
                     /label=BBa_B0012
     stem_loop       2894..2916
                     /label=stem_loop
     misc_feature    2882..2927
                     /label=BBa_B0011
     terminator      2882..2927
                     /label=BBa_B0011
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatggcttc caaggtgtac gaccccgagc aacgcaaacg catgatcact gggcctcagt
      121 ggtgggctcg ctgcaagcaa atgaacgtgc tggactcctt catcaactac tatgattccg
      181 agaagcacgc cgagaacgcc gtgatttttc tgcatggtaa cgctgcctcc agctacctgt
      241 ggaggcacgt cgtgcctcac atcgagcccg tggctagatg catcatccct gatctgatcg
      301 gaatgggtaa gtccggcaag agcgggaatg gctcatatcg cctcctggat cactacaagt
      361 acctcaccgc ttggttcgag ctgctgaacc ttccaaagaa aatcatcttt gtgggccacg
      421 actggggggc ttgtctggcc tttcactact cctacgagca ccaagacaag atcaaggcca
      481 tcgtccatgc tgagagtgtc gtggacgtga tcgagtcctg ggacgagtgg cctgacatcg
      541 aggaggatat cgccctgatc aagagcgaag agggcgagaa aatggtgctt gagaataact
      601 tcttcgtcga gaccatgctc ccaagcaaga tcatgcggaa actggagcct gaggagttcg
      661 ctgcctacct ggagccattc aaggagaagg gcgaggttag acggcctacc ctctcctggc
      721 ctcgcgagat ccctctcgtt aagggaggca agcccgacgt cgtccagatt gtccgcaact
      781 acaacgccta ccttcgggcc agcgacgatc tgcctaagat gttcatcgag tccgaccctg
      841 ggttcttttc caacgctatt gtcgagggag ctaagaagtt ccctaacacc gagttcgtga
      901 aggtgaaggg cctccacttc agccaggagg acgctccaga tgaaatgggt aagtacatca
      961 agagcttcgt ggagcgcgtg ctgaagaacg agcagtaata ctagagtcac actggctcac
     1021 cttcgggtgg gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt
     1081 aatccggctt ttttattatt ttactagagt ttacagctag ctcagtccta ggtattatgc
     1141 tagctactag agtcacacag gaaagtacta gatggaagac gccaaaaaca taaagaaagg
     1201 cccggcgcca ttctatccgc tggaagatgg aaccgctgga gagcaactgc ataaggctat
     1261 gaagagatac gccctggttc ctggaacaat tgcttttaca gatgcacata tcgaggtgga
     1321 catcacttac gctgagtact tcgaaatgtc cgttcggttg gcagaagcta tgaaacgata
     1381 tgggctgaat acaaatcaca gaatcgtcgt atgcagtgaa aactctcttc aattctttat
     1441 gccggtgttg ggcgcgttat ttatcggagt tgcagttgcg cccgcgaacg acatttataa
     1501 tgaacgtgaa ttgctcaaca gtatgggcat ttcgcagcct accgtggtgt tcgtttccaa
     1561 aaaggggttg caaaaaattt tgaacgtgca aaaaaagctc ccaatcatcc aaaaaattat
     1621 tatcatggat tctaaaacgg attaccaggg atttcagtcg atgtacacgt tcgtcacatc
     1681 tcatctacct cccggtttta atgaatacga ttttgtgcca gagtccttcg atagggacaa
     1741 gacaattgca ctgatcatga actcctctgg atctactggt ctgcctaaag gtgtcgctct
     1801 gcctcataga actgcctgcg tgagattctc gcatgccaga gatcctattt ttggcaatca
     1861 aatcattccg gatactgcga ttttaagtgt tgttccattc catcacggtt ttggaatgtt
     1921 tactacactc ggatatttga tatgtggatt tcgagtcgtc ttaatgtata gatttgaaga
     1981 agagctgttt ctgaggagcc ttcaggatta caagattcaa agtgcgctgc tggtgccaac
     2041 cctattctcc ttcttcgcca aaagcactct gattgacaaa tacgatttat ctaatttaca
     2101 cgaaattgct tctggtggcg ctcccctctc taaggaagtc ggggaagcgg ttgccaagag
     2161 gttccatctg ccaggtatca ggcaaggata tgggctcact gagactacat cagctattct
     2221 gattacaccc gagggggatg ataaaccggg cgcggtcggt aaagttgttc cattttttga
     2281 agcgaaggtt gtggatctgg ataccgggaa aacgctgggc gttaatcaaa gaggcgaact
     2341 gtgtgtgaga ggtcctatga ttatgtccgg ttatgtaaac aatccggaag cgaccaacgc
     2401 cttgattgac aaggatggat ggctacattc tggagacata gcttactggg acgaagacga
     2461 acacttcttc atcgttgacc gcctgaagtc tctgattaag tacaaaggct atcaggtggc
     2521 tcccgctgaa ttggaatcca tcttgctcca acaccccaac atcttcgacg caggtgtcgc
     2581 aggtcttccc gacgatgacg ccggtgaact tcccgccgcc gttgttgttt tggagcacgg
     2641 aaagacgatg acggaaaaag agatcgtgga ttacgtcgcc agtcaagtaa caaccgcgaa
     2701 aaagttgcgc ggaggagttg tgtttgtgga cgaagtaccg aaaggtctta ccggaaaact
     2761 cgacgcaaga aaaatcagag agatcctcat aaaggccaag aagggcggaa agatcgccgt
     2821 gtaatactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga
     2881 gagagaatat aaaaagccag attattaatc cggctttttt attattt
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