LOCUS       BBa_J95014              2781 bp    DNA     linear       25-Nov-2024
DEFINITION  Bioluminescence based terminator measurement kit
ACCESSION   BBa_J95014
VERSION     BBa_J95014.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     misc_feature    63..1715
                     /label=BBa_J95000
     RBS             1724..1736
                     /label=RBS-3\Weak
     RBS             1730..1733
                     /label=RBS
     misc_feature    1724..1736
                     /label=BBa_B0032
     RBS             1724..1736
                     /label=BBa_B0032
     misc_feature    1743..2678
                     /label=BBa_J95001
     stem_loop       2694..2713
                     /label=T7 TE
     misc_feature    2720..2720
                     /label=stop
     polyA_site      2714..2727
                     /label=polya
     misc_feature    2687..2727
                     /label=BBa_B0012
     terminator      2687..2727
                     /label=BBa_B0012
     stem_loop       2748..2770
                     /label=stem_loop
     misc_feature    2736..2781
                     /label=BBa_B0011
     terminator      2736..2781
                     /label=BBa_B0011
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg
      121 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
      181 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt
      241 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
      301 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
      361 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca
      421 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
      481 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg
      541 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
      601 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg
      661 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct
      721 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
      781 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
      841 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt
      901 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc
      961 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct
     1021 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat
     1081 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
     1141 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
     1201 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg
     1261 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
     1321 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt
     1381 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc
     1441 aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac
     1501 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
     1561 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
     1621 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
     1681 taaaggccaa gaagggcgga aagatcgccg tgtaatacta gagtcacaca ggaaagtact
     1741 agatggcttc caaggtgtac gaccccgagc aacgcaaacg catgatcact gggcctcagt
     1801 ggtgggctcg ctgcaagcaa atgaacgtgc tggactcctt catcaactac tatgattccg
     1861 agaagcacgc cgagaacgcc gtgatttttc tgcatggtaa cgctgcctcc agctacctgt
     1921 ggaggcacgt cgtgcctcac atcgagcccg tggctagatg catcatccct gatctgatcg
     1981 gaatgggtaa gtccggcaag agcgggaatg gctcatatcg cctcctggat cactacaagt
     2041 acctcaccgc ttggttcgag ctgctgaacc ttccaaagaa aatcatcttt gtgggccacg
     2101 actggggggc ttgtctggcc tttcactact cctacgagca ccaagacaag atcaaggcca
     2161 tcgtccatgc tgagagtgtc gtggacgtga tcgagtcctg ggacgagtgg cctgacatcg
     2221 aggaggatat cgccctgatc aagagcgaag agggcgagaa aatggtgctt gagaataact
     2281 tcttcgtcga gaccatgctc ccaagcaaga tcatgcggaa actggagcct gaggagttcg
     2341 ctgcctacct ggagccattc aaggagaagg gcgaggttag acggcctacc ctctcctggc
     2401 ctcgcgagat ccctctcgtt aagggaggca agcccgacgt cgtccagatt gtccgcaact
     2461 acaacgccta ccttcgggcc agcgacgatc tgcctaagat gttcatcgag tccgaccctg
     2521 ggttcttttc caacgctatt gtcgagggag ctaagaagtt ccctaacacc gagttcgtga
     2581 aggtgaaggg cctccacttc agccaggagg acgctccaga tgaaatgggt aagtacatca
     2641 agagcttcgt ggagcgcgtg ctgaagaacg agcagtaata ctagagtcac actggctcac
     2701 cttcgggtgg gcctttctgc gtttatatac tagagagaga atataaaaag ccagattatt
     2761 aatccggctt ttttattatt t
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