LOCUS       BBa_J95040              2918 bp    DNA     linear       22-Dec-2024
DEFINITION  B0015 characterization construct
ACCESSION   BBa_J95040
VERSION     BBa_J95040.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     misc_feature    63..1715
                     /label=BBa_J95000
     stem_loop       1735..1778
                     /label=stem_loop
     misc_feature    1724..1803
                     /label=BBa_B0010
     terminator      1724..1803
                     /label=BBa_B0010
     stem_loop       1819..1838
                     /label=T7 TE
     misc_feature    1845..1845
                     /label=stop
     polyA_site      1839..1852
                     /label=polya
     misc_feature    1812..1852
                     /label=BBa_B0012
     terminator      1812..1852
                     /label=BBa_B0012
     RBS             1861..1873
                     /label=RBS-3\Weak
     RBS             1867..1870
                     /label=RBS
     misc_feature    1861..1873
                     /label=BBa_B0032
     RBS             1861..1873
                     /label=BBa_B0032
     misc_feature    1880..2815
                     /label=BBa_J95001
     stem_loop       2831..2850
                     /label=T7 TE
     misc_feature    2857..2857
                     /label=stop
     polyA_site      2851..2864
                     /label=polya
     misc_feature    2824..2864
                     /label=BBa_B0012
     terminator      2824..2864
                     /label=BBa_B0012
     stem_loop       2885..2907
                     /label=stem_loop
     misc_feature    2873..2918
                     /label=BBa_B0011
     terminator      2873..2918
                     /label=BBa_B0011
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg
      121 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
      181 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt
      241 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
      301 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
      361 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca
      421 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
      481 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg
      541 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
      601 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg
      661 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct
      721 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
      781 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
      841 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt
      901 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc
      961 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct
     1021 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat
     1081 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
     1141 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
     1201 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg
     1261 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
     1321 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt
     1381 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc
     1441 aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac
     1501 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
     1561 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
     1621 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
     1681 taaaggccaa gaagggcgga aagatcgccg tgtaatacta gagccaggca tcaaataaaa
     1741 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1801 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
     1861 tcacacagga aagtactaga tggcttccaa ggtgtacgac cccgagcaac gcaaacgcat
     1921 gatcactggg cctcagtggt gggctcgctg caagcaaatg aacgtgctgg actccttcat
     1981 caactactat gattccgaga agcacgccga gaacgccgtg atttttctgc atggtaacgc
     2041 tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc gagcccgtgg ctagatgcat
     2101 catccctgat ctgatcggaa tgggtaagtc cggcaagagc gggaatggct catatcgcct
     2161 cctggatcac tacaagtacc tcaccgcttg gttcgagctg ctgaaccttc caaagaaaat
     2221 catctttgtg ggccacgact ggggggcttg tctggccttt cactactcct acgagcacca
     2281 agacaagatc aaggccatcg tccatgctga gagtgtcgtg gacgtgatcg agtcctggga
     2341 cgagtggcct gacatcgagg aggatatcgc cctgatcaag agcgaagagg gcgagaaaat
     2401 ggtgcttgag aataacttct tcgtcgagac catgctccca agcaagatca tgcggaaact
     2461 ggagcctgag gagttcgctg cctacctgga gccattcaag gagaagggcg aggttagacg
     2521 gcctaccctc tcctggcctc gcgagatccc tctcgttaag ggaggcaagc ccgacgtcgt
     2581 ccagattgtc cgcaactaca acgcctacct tcgggccagc gacgatctgc ctaagatgtt
     2641 catcgagtcc gaccctgggt tcttttccaa cgctattgtc gagggagcta agaagttccc
     2701 taacaccgag ttcgtgaagg tgaagggcct ccacttcagc caggaggacg ctccagatga
     2761 aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg aagaacgagc agtaatacta
     2821 gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag agagagaata
     2881 taaaaagcca gattattaat ccggcttttt tattattt
//