LOCUS       BBa_J95042              2829 bp    DNA     linear       22-Nov-2024
DEFINITION  Promoter measurement standard (B1009) for E coli
ACCESSION   BBa_J95042
VERSION     BBa_J95042.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     misc_feature    63..1715
                     /label=BBa_J95000
     misc_feature    1724..1763
                     /label=B1009
     stem_loop       1733..1754
                     /label=stem loop
     polyA_site      1729..1732
                     /label=PolyA
     polyA_site      1755..1758
                     /label=PolyA
     terminator      1724..1763
                     /label=BBa_B1009
     RBS             1772..1784
                     /label=RBS-3\Weak
     RBS             1778..1781
                     /label=RBS
     misc_feature    1772..1784
                     /label=BBa_B0032
     RBS             1772..1784
                     /label=BBa_B0032
     misc_feature    1791..2726
                     /label=BBa_J95001
     stem_loop       2742..2761
                     /label=T7 TE
     misc_feature    2768..2768
                     /label=stop
     polyA_site      2762..2775
                     /label=polya
     misc_feature    2735..2775
                     /label=BBa_B0012
     terminator      2735..2775
                     /label=BBa_B0012
     stem_loop       2796..2818
                     /label=stem_loop
     misc_feature    2784..2829
                     /label=BBa_B0011
     terminator      2784..2829
                     /label=BBa_B0011
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg
      121 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
      181 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt
      241 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
      301 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
      361 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca
      421 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
      481 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg
      541 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
      601 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg
      661 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct
      721 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
      781 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
      841 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt
      901 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc
      961 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct
     1021 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat
     1081 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
     1141 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
     1201 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg
     1261 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
     1321 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt
     1381 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc
     1441 aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac
     1501 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
     1561 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
     1621 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
     1681 taaaggccaa gaagggcgga aagatcgccg tgtaatacta gagcgccgaa aaccccgccc
     1741 ctgacagggc ggggttttgc cgctactaga gtcacacagg aaagtactag atggcttcca
     1801 aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg tgggctcgct
     1861 gcaagcaaat gaacgtgctg gactccttca tcaactacta tgattccgag aagcacgccg
     1921 agaacgccgt gatttttctg catggtaacg ctgcctccag ctacctgtgg aggcacgtcg
     1981 tgcctcacat cgagcccgtg gctagatgca tcatccctga tctgatcgga atgggtaagt
     2041 ccggcaagag cgggaatggc tcatatcgcc tcctggatca ctacaagtac ctcaccgctt
     2101 ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt gggccacgac tggggggctt
     2161 gtctggcctt tcactactcc tacgagcacc aagacaagat caaggccatc gtccatgctg
     2221 agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc tgacatcgag gaggatatcg
     2281 ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga gaataacttc ttcgtcgaga
     2341 ccatgctccc aagcaagatc atgcggaaac tggagcctga ggagttcgct gcctacctgg
     2401 agccattcaa ggagaagggc gaggttagac ggcctaccct ctcctggcct cgcgagatcc
     2461 ctctcgttaa gggaggcaag cccgacgtcg tccagattgt ccgcaactac aacgcctacc
     2521 ttcgggccag cgacgatctg cctaagatgt tcatcgagtc cgaccctggg ttcttttcca
     2581 acgctattgt cgagggagct aagaagttcc ctaacaccga gttcgtgaag gtgaagggcc
     2641 tccacttcag ccaggaggac gctccagatg aaatgggtaa gtacatcaag agcttcgtgg
     2701 agcgcgtgct gaagaacgag cagtaatact agagtcacac tggctcacct tcgggtgggc
     2761 ctttctgcgt ttatatacta gagagagaat ataaaaagcc agattattaa tccggctttt
     2821 ttattattt
//