LOCUS       BBa_J95043              2952 bp    DNA     linear       15-Jun-2024
DEFINITION  Promoter measurement kit A (B0015) for E coli
ACCESSION   BBa_J95043
VERSION     BBa_J95043.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     misc_feature    20..1672
                     /label=BBa_J95000
     stem_loop       1692..1735
                     /label=stem_loop
     misc_feature    1681..1760
                     /label=BBa_B0010
     terminator      1681..1760
                     /label=BBa_B0010
     stem_loop       1776..1795
                     /label=T7 TE
     misc_feature    1802..1802
                     /label=stop
     polyA_site      1796..1809
                     /label=polya
     misc_feature    1769..1809
                     /label=BBa_B0012
     terminator      1769..1809
                     /label=BBa_B0012
     terminator      1681..1809
                     /label=BBa_B0015
     promoter        1818..1852
                     /label=BBa_J23101
     RBS             1861..1873
                     /label=RBS-3\Weak
     RBS             1867..1870
                     /label=RBS
     misc_feature    1861..1873
                     /label=BBa_B0032
     RBS             1861..1873
                     /label=BBa_B0032
     misc_feature    1880..2815
                     /label=BBa_J95001
     stem_loop       2835..2878
                     /label=stem_loop
     misc_feature    2824..2903
                     /label=BBa_B0010
     terminator      2824..2903
                     /label=BBa_B0010
     stem_loop       2919..2938
                     /label=T7 TE
     misc_feature    2945..2945
                     /label=stop
     polyA_site      2939..2952
                     /label=polya
     misc_feature    2912..2952
                     /label=BBa_B0012
     terminator      2912..2952
                     /label=BBa_B0012
     terminator      2824..2952
                     /label=BBa_B0015
ORIGIN
        1 tcacacagga aagtactaga tggaagacgc caaaaacata aagaaaggcc cggcgccatt
       61 ctatccgctg gaagatggaa ccgctggaga gcaactgcat aaggctatga agagatacgc
      121 cctggttcct ggaacaattg cttttacaga tgcacatatc gaggtggaca tcacttacgc
      181 tgagtacttc gaaatgtccg ttcggttggc agaagctatg aaacgatatg ggctgaatac
      241 aaatcacaga atcgtcgtat gcagtgaaaa ctctcttcaa ttctttatgc cggtgttggg
      301 cgcgttattt atcggagttg cagttgcgcc cgcgaacgac atttataatg aacgtgaatt
      361 gctcaacagt atgggcattt cgcagcctac cgtggtgttc gtttccaaaa aggggttgca
      421 aaaaattttg aacgtgcaaa aaaagctccc aatcatccaa aaaattatta tcatggattc
      481 taaaacggat taccagggat ttcagtcgat gtacacgttc gtcacatctc atctacctcc
      541 cggttttaat gaatacgatt ttgtgccaga gtccttcgat agggacaaga caattgcact
      601 gatcatgaac tcctctggat ctactggtct gcctaaaggt gtcgctctgc ctcatagaac
      661 tgcctgcgtg agattctcgc atgccagaga tcctattttt ggcaatcaaa tcattccgga
      721 tactgcgatt ttaagtgttg ttccattcca tcacggtttt ggaatgttta ctacactcgg
      781 atatttgata tgtggatttc gagtcgtctt aatgtataga tttgaagaag agctgtttct
      841 gaggagcctt caggattaca agattcaaag tgcgctgctg gtgccaaccc tattctcctt
      901 cttcgccaaa agcactctga ttgacaaata cgatttatct aatttacacg aaattgcttc
      961 tggtggcgct cccctctcta aggaagtcgg ggaagcggtt gccaagaggt tccatctgcc
     1021 aggtatcagg caaggatatg ggctcactga gactacatca gctattctga ttacacccga
     1081 gggggatgat aaaccgggcg cggtcggtaa agttgttcca ttttttgaag cgaaggttgt
     1141 ggatctggat accgggaaaa cgctgggcgt taatcaaaga ggcgaactgt gtgtgagagg
     1201 tcctatgatt atgtccggtt atgtaaacaa tccggaagcg accaacgcct tgattgacaa
     1261 ggatggatgg ctacattctg gagacatagc ttactgggac gaagacgaac acttcttcat
     1321 cgttgaccgc ctgaagtctc tgattaagta caaaggctat caggtggctc ccgctgaatt
     1381 ggaatccatc ttgctccaac accccaacat cttcgacgca ggtgtcgcag gtcttcccga
     1441 cgatgacgcc ggtgaacttc ccgccgccgt tgttgttttg gagcacggaa agacgatgac
     1501 ggaaaaagag atcgtggatt acgtcgccag tcaagtaaca accgcgaaaa agttgcgcgg
     1561 aggagttgtg tttgtggacg aagtaccgaa aggtcttacc ggaaaactcg acgcaagaaa
     1621 aatcagagag atcctcataa aggccaagaa gggcggaaag atcgccgtgt aatactagag
     1681 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
     1741 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
     1801 gcgtttatat actagagttt acagctagct cagtcctagg tattatgcta gctactagag
     1861 tcacacagga aagtactaga tggcttccaa ggtgtacgac cccgagcaac gcaaacgcat
     1921 gatcactggg cctcagtggt gggctcgctg caagcaaatg aacgtgctgg actccttcat
     1981 caactactat gattccgaga agcacgccga gaacgccgtg atttttctgc atggtaacgc
     2041 tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc gagcccgtgg ctagatgcat
     2101 catccctgat ctgatcggaa tgggtaagtc cggcaagagc gggaatggct catatcgcct
     2161 cctggatcac tacaagtacc tcaccgcttg gttcgagctg ctgaaccttc caaagaaaat
     2221 catctttgtg ggccacgact ggggggcttg tctggccttt cactactcct acgagcacca
     2281 agacaagatc aaggccatcg tccatgctga gagtgtcgtg gacgtgatcg agtcctggga
     2341 cgagtggcct gacatcgagg aggatatcgc cctgatcaag agcgaagagg gcgagaaaat
     2401 ggtgcttgag aataacttct tcgtcgagac catgctccca agcaagatca tgcggaaact
     2461 ggagcctgag gagttcgctg cctacctgga gccattcaag gagaagggcg aggttagacg
     2521 gcctaccctc tcctggcctc gcgagatccc tctcgttaag ggaggcaagc ccgacgtcgt
     2581 ccagattgtc cgcaactaca acgcctacct tcgggccagc gacgatctgc ctaagatgtt
     2641 catcgagtcc gaccctgggt tcttttccaa cgctattgtc gagggagcta agaagttccc
     2701 taacaccgag ttcgtgaagg tgaagggcct ccacttcagc caggaggacg ctccagatga
     2761 aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg aagaacgagc agtaatacta
     2821 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     2881 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     2941 tctgcgttta ta
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