LOCUS       BBa_J95044              2952 bp    DNA     linear       15-Jun-2024
DEFINITION  Promoter measurement kit B (B0015) for E coli
ACCESSION   BBa_J95044
VERSION     BBa_J95044.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     misc_feature    20..955
                     /label=BBa_J95001
     stem_loop       975..1018
                     /label=stem_loop
     misc_feature    964..1043
                     /label=BBa_B0010
     terminator      964..1043
                     /label=BBa_B0010
     stem_loop       1059..1078
                     /label=T7 TE
     misc_feature    1085..1085
                     /label=stop
     polyA_site      1079..1092
                     /label=polya
     misc_feature    1052..1092
                     /label=BBa_B0012
     terminator      1052..1092
                     /label=BBa_B0012
     terminator      964..1092
                     /label=BBa_B0015
     promoter        1101..1135
                     /label=BBa_J23101
     RBS             1144..1156
                     /label=RBS-3\Weak
     RBS             1150..1153
                     /label=RBS
     misc_feature    1144..1156
                     /label=BBa_B0032
     RBS             1144..1156
                     /label=BBa_B0032
     misc_feature    1163..2815
                     /label=BBa_J95000
     stem_loop       2835..2878
                     /label=stem_loop
     misc_feature    2824..2903
                     /label=BBa_B0010
     terminator      2824..2903
                     /label=BBa_B0010
     stem_loop       2919..2938
                     /label=T7 TE
     misc_feature    2945..2945
                     /label=stop
     polyA_site      2939..2952
                     /label=polya
     misc_feature    2912..2952
                     /label=BBa_B0012
     terminator      2912..2952
                     /label=BBa_B0012
     terminator      2824..2952
                     /label=BBa_B0015
ORIGIN
        1 tcacacagga aagtactaga tggcttccaa ggtgtacgac cccgagcaac gcaaacgcat
       61 gatcactggg cctcagtggt gggctcgctg caagcaaatg aacgtgctgg actccttcat
      121 caactactat gattccgaga agcacgccga gaacgccgtg atttttctgc atggtaacgc
      181 tgcctccagc tacctgtgga ggcacgtcgt gcctcacatc gagcccgtgg ctagatgcat
      241 catccctgat ctgatcggaa tgggtaagtc cggcaagagc gggaatggct catatcgcct
      301 cctggatcac tacaagtacc tcaccgcttg gttcgagctg ctgaaccttc caaagaaaat
      361 catctttgtg ggccacgact ggggggcttg tctggccttt cactactcct acgagcacca
      421 agacaagatc aaggccatcg tccatgctga gagtgtcgtg gacgtgatcg agtcctggga
      481 cgagtggcct gacatcgagg aggatatcgc cctgatcaag agcgaagagg gcgagaaaat
      541 ggtgcttgag aataacttct tcgtcgagac catgctccca agcaagatca tgcggaaact
      601 ggagcctgag gagttcgctg cctacctgga gccattcaag gagaagggcg aggttagacg
      661 gcctaccctc tcctggcctc gcgagatccc tctcgttaag ggaggcaagc ccgacgtcgt
      721 ccagattgtc cgcaactaca acgcctacct tcgggccagc gacgatctgc ctaagatgtt
      781 catcgagtcc gaccctgggt tcttttccaa cgctattgtc gagggagcta agaagttccc
      841 taacaccgag ttcgtgaagg tgaagggcct ccacttcagc caggaggacg ctccagatga
      901 aatgggtaag tacatcaaga gcttcgtgga gcgcgtgctg aagaacgagc agtaatacta
      961 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1021 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1081 tctgcgttta tatactagag tttacagcta gctcagtcct aggtattatg ctagctacta
     1141 gagtcacaca ggaaagtact agatggaaga cgccaaaaac ataaagaaag gcccggcgcc
     1201 attctatccg ctggaagatg gaaccgctgg agagcaactg cataaggcta tgaagagata
     1261 cgccctggtt cctggaacaa ttgcttttac agatgcacat atcgaggtgg acatcactta
     1321 cgctgagtac ttcgaaatgt ccgttcggtt ggcagaagct atgaaacgat atgggctgaa
     1381 tacaaatcac agaatcgtcg tatgcagtga aaactctctt caattcttta tgccggtgtt
     1441 gggcgcgtta tttatcggag ttgcagttgc gcccgcgaac gacatttata atgaacgtga
     1501 attgctcaac agtatgggca tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt
     1561 gcaaaaaatt ttgaacgtgc aaaaaaagct cccaatcatc caaaaaatta ttatcatgga
     1621 ttctaaaacg gattaccagg gatttcagtc gatgtacacg ttcgtcacat ctcatctacc
     1681 tcccggtttt aatgaatacg attttgtgcc agagtccttc gatagggaca agacaattgc
     1741 actgatcatg aactcctctg gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag
     1801 aactgcctgc gtgagattct cgcatgccag agatcctatt tttggcaatc aaatcattcc
     1861 ggatactgcg attttaagtg ttgttccatt ccatcacggt tttggaatgt ttactacact
     1921 cggatatttg atatgtggat ttcgagtcgt cttaatgtat agatttgaag aagagctgtt
     1981 tctgaggagc cttcaggatt acaagattca aagtgcgctg ctggtgccaa ccctattctc
     2041 cttcttcgcc aaaagcactc tgattgacaa atacgattta tctaatttac acgaaattgc
     2101 ttctggtggc gctcccctct ctaaggaagt cggggaagcg gttgccaaga ggttccatct
     2161 gccaggtatc aggcaaggat atgggctcac tgagactaca tcagctattc tgattacacc
     2221 cgagggggat gataaaccgg gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt
     2281 tgtggatctg gataccggga aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag
     2341 aggtcctatg attatgtccg gttatgtaaa caatccggaa gcgaccaacg ccttgattga
     2401 caaggatgga tggctacatt ctggagacat agcttactgg gacgaagacg aacacttctt
     2461 catcgttgac cgcctgaagt ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga
     2521 attggaatcc atcttgctcc aacaccccaa catcttcgac gcaggtgtcg caggtcttcc
     2581 cgacgatgac gccggtgaac ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat
     2641 gacggaaaaa gagatcgtgg attacgtcgc cagtcaagta acaaccgcga aaaagttgcg
     2701 cggaggagtt gtgtttgtgg acgaagtacc gaaaggtctt accggaaaac tcgacgcaag
     2761 aaaaatcaga gagatcctca taaaggccaa gaagggcgga aagatcgccg tgtaatacta
     2821 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     2881 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     2941 tctgcgttta ta
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