LOCUS       BBa_J95045              2995 bp    DNA     linear       22-Nov-2024
DEFINITION  reference standard for Promoter measurement (B0015) for E coli
ACCESSION   BBa_J95045
VERSION     BBa_J95045.1  
FEATURES             Location/Qualifiers
     promoter        1..35
                     /label=BBa_J23101
     RBS             44..56
                     /label=RBS-3\Weak
     RBS             50..53
                     /label=RBS
     misc_feature    44..56
                     /label=BBa_B0032
     RBS             44..56
                     /label=BBa_B0032
     misc_feature    63..1715
                     /label=BBa_J95000
     stem_loop       1735..1778
                     /label=stem_loop
     misc_feature    1724..1803
                     /label=BBa_B0010
     terminator      1724..1803
                     /label=BBa_B0010
     stem_loop       1819..1838
                     /label=T7 TE
     misc_feature    1845..1845
                     /label=stop
     polyA_site      1839..1852
                     /label=polya
     misc_feature    1812..1852
                     /label=BBa_B0012
     terminator      1812..1852
                     /label=BBa_B0012
     terminator      1724..1852
                     /label=BBa_B0015
     promoter        1861..1895
                     /label=BBa_J23101
     RBS             1904..1916
                     /label=RBS-3\Weak
     RBS             1910..1913
                     /label=RBS
     misc_feature    1904..1916
                     /label=BBa_B0032
     RBS             1904..1916
                     /label=BBa_B0032
     misc_feature    1923..2858
                     /label=BBa_J95001
     stem_loop       2878..2921
                     /label=stem_loop
     misc_feature    2867..2946
                     /label=BBa_B0010
     terminator      2867..2946
                     /label=BBa_B0010
     stem_loop       2962..2981
                     /label=T7 TE
     misc_feature    2988..2988
                     /label=stop
     polyA_site      2982..2995
                     /label=polya
     misc_feature    2955..2995
                     /label=BBa_B0012
     terminator      2955..2995
                     /label=BBa_B0012
     terminator      2867..2995
                     /label=BBa_B0015
ORIGIN
        1 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
       61 agatggaaga cgccaaaaac ataaagaaag gcccggcgcc attctatccg ctggaagatg
      121 gaaccgctgg agagcaactg cataaggcta tgaagagata cgccctggtt cctggaacaa
      181 ttgcttttac agatgcacat atcgaggtgg acatcactta cgctgagtac ttcgaaatgt
      241 ccgttcggtt ggcagaagct atgaaacgat atgggctgaa tacaaatcac agaatcgtcg
      301 tatgcagtga aaactctctt caattcttta tgccggtgtt gggcgcgtta tttatcggag
      361 ttgcagttgc gcccgcgaac gacatttata atgaacgtga attgctcaac agtatgggca
      421 tttcgcagcc taccgtggtg ttcgtttcca aaaaggggtt gcaaaaaatt ttgaacgtgc
      481 aaaaaaagct cccaatcatc caaaaaatta ttatcatgga ttctaaaacg gattaccagg
      541 gatttcagtc gatgtacacg ttcgtcacat ctcatctacc tcccggtttt aatgaatacg
      601 attttgtgcc agagtccttc gatagggaca agacaattgc actgatcatg aactcctctg
      661 gatctactgg tctgcctaaa ggtgtcgctc tgcctcatag aactgcctgc gtgagattct
      721 cgcatgccag agatcctatt tttggcaatc aaatcattcc ggatactgcg attttaagtg
      781 ttgttccatt ccatcacggt tttggaatgt ttactacact cggatatttg atatgtggat
      841 ttcgagtcgt cttaatgtat agatttgaag aagagctgtt tctgaggagc cttcaggatt
      901 acaagattca aagtgcgctg ctggtgccaa ccctattctc cttcttcgcc aaaagcactc
      961 tgattgacaa atacgattta tctaatttac acgaaattgc ttctggtggc gctcccctct
     1021 ctaaggaagt cggggaagcg gttgccaaga ggttccatct gccaggtatc aggcaaggat
     1081 atgggctcac tgagactaca tcagctattc tgattacacc cgagggggat gataaaccgg
     1141 gcgcggtcgg taaagttgtt ccattttttg aagcgaaggt tgtggatctg gataccggga
     1201 aaacgctggg cgttaatcaa agaggcgaac tgtgtgtgag aggtcctatg attatgtccg
     1261 gttatgtaaa caatccggaa gcgaccaacg ccttgattga caaggatgga tggctacatt
     1321 ctggagacat agcttactgg gacgaagacg aacacttctt catcgttgac cgcctgaagt
     1381 ctctgattaa gtacaaaggc tatcaggtgg ctcccgctga attggaatcc atcttgctcc
     1441 aacaccccaa catcttcgac gcaggtgtcg caggtcttcc cgacgatgac gccggtgaac
     1501 ttcccgccgc cgttgttgtt ttggagcacg gaaagacgat gacggaaaaa gagatcgtgg
     1561 attacgtcgc cagtcaagta acaaccgcga aaaagttgcg cggaggagtt gtgtttgtgg
     1621 acgaagtacc gaaaggtctt accggaaaac tcgacgcaag aaaaatcaga gagatcctca
     1681 taaaggccaa gaagggcgga aagatcgccg tgtaatacta gagccaggca tcaaataaaa
     1741 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct
     1801 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta tatactagag
     1861 tttacagcta gctcagtcct aggtattatg ctagctacta gagtcacaca ggaaagtact
     1921 agatggcttc caaggtgtac gaccccgagc aacgcaaacg catgatcact gggcctcagt
     1981 ggtgggctcg ctgcaagcaa atgaacgtgc tggactcctt catcaactac tatgattccg
     2041 agaagcacgc cgagaacgcc gtgatttttc tgcatggtaa cgctgcctcc agctacctgt
     2101 ggaggcacgt cgtgcctcac atcgagcccg tggctagatg catcatccct gatctgatcg
     2161 gaatgggtaa gtccggcaag agcgggaatg gctcatatcg cctcctggat cactacaagt
     2221 acctcaccgc ttggttcgag ctgctgaacc ttccaaagaa aatcatcttt gtgggccacg
     2281 actggggggc ttgtctggcc tttcactact cctacgagca ccaagacaag atcaaggcca
     2341 tcgtccatgc tgagagtgtc gtggacgtga tcgagtcctg ggacgagtgg cctgacatcg
     2401 aggaggatat cgccctgatc aagagcgaag agggcgagaa aatggtgctt gagaataact
     2461 tcttcgtcga gaccatgctc ccaagcaaga tcatgcggaa actggagcct gaggagttcg
     2521 ctgcctacct ggagccattc aaggagaagg gcgaggttag acggcctacc ctctcctggc
     2581 ctcgcgagat ccctctcgtt aagggaggca agcccgacgt cgtccagatt gtccgcaact
     2641 acaacgccta ccttcgggcc agcgacgatc tgcctaagat gttcatcgag tccgaccctg
     2701 ggttcttttc caacgctatt gtcgagggag ctaagaagtt ccctaacacc gagttcgtga
     2761 aggtgaaggg cctccacttc agccaggagg acgctccaga tgaaatgggt aagtacatca
     2821 agagcttcgt ggagcgcgtg ctgaagaacg agcagtaata ctagagccag gcatcaaata
     2881 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     2941 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
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