LOCUS       BBa_J95046              2933 bp    DNA     linear       22-Nov-2024
DEFINITION  One of two RBS measurement kits A(B0015)
ACCESSION   BBa_J95046
VERSION     BBa_J95046.1  
FEATURES             Location/Qualifiers
     misc_feature    1..1653
                     /label=BBa_J95000
     stem_loop       1673..1716
                     /label=stem_loop
     misc_feature    1662..1741
                     /label=BBa_B0010
     terminator      1662..1741
                     /label=BBa_B0010
     stem_loop       1757..1776
                     /label=T7 TE
     misc_feature    1783..1783
                     /label=stop
     polyA_site      1777..1790
                     /label=polya
     misc_feature    1750..1790
                     /label=BBa_B0012
     terminator      1750..1790
                     /label=BBa_B0012
     terminator      1662..1790
                     /label=BBa_B0015
     promoter        1799..1833
                     /label=BBa_J23101
     RBS             1842..1854
                     /label=RBS-3\Weak
     RBS             1848..1851
                     /label=RBS
     misc_feature    1842..1854
                     /label=BBa_B0032
     RBS             1842..1854
                     /label=BBa_B0032
     misc_feature    1861..2796
                     /label=BBa_J95001
     stem_loop       2816..2859
                     /label=stem_loop
     misc_feature    2805..2884
                     /label=BBa_B0010
     terminator      2805..2884
                     /label=BBa_B0010
     stem_loop       2900..2919
                     /label=T7 TE
     misc_feature    2926..2926
                     /label=stop
     polyA_site      2920..2933
                     /label=polya
     misc_feature    2893..2933
                     /label=BBa_B0012
     terminator      2893..2933
                     /label=BBa_B0012
     terminator      2805..2933
                     /label=BBa_B0015
ORIGIN
        1 atggaagacg ccaaaaacat aaagaaaggc ccggcgccat tctatccgct ggaagatgga
       61 accgctggag agcaactgca taaggctatg aagagatacg ccctggttcc tggaacaatt
      121 gcttttacag atgcacatat cgaggtggac atcacttacg ctgagtactt cgaaatgtcc
      181 gttcggttgg cagaagctat gaaacgatat gggctgaata caaatcacag aatcgtcgta
      241 tgcagtgaaa actctcttca attctttatg ccggtgttgg gcgcgttatt tatcggagtt
      301 gcagttgcgc ccgcgaacga catttataat gaacgtgaat tgctcaacag tatgggcatt
      361 tcgcagccta ccgtggtgtt cgtttccaaa aaggggttgc aaaaaatttt gaacgtgcaa
      421 aaaaagctcc caatcatcca aaaaattatt atcatggatt ctaaaacgga ttaccaggga
      481 tttcagtcga tgtacacgtt cgtcacatct catctacctc ccggttttaa tgaatacgat
      541 tttgtgccag agtccttcga tagggacaag acaattgcac tgatcatgaa ctcctctgga
      601 tctactggtc tgcctaaagg tgtcgctctg cctcatagaa ctgcctgcgt gagattctcg
      661 catgccagag atcctatttt tggcaatcaa atcattccgg atactgcgat tttaagtgtt
      721 gttccattcc atcacggttt tggaatgttt actacactcg gatatttgat atgtggattt
      781 cgagtcgtct taatgtatag atttgaagaa gagctgtttc tgaggagcct tcaggattac
      841 aagattcaaa gtgcgctgct ggtgccaacc ctattctcct tcttcgccaa aagcactctg
      901 attgacaaat acgatttatc taatttacac gaaattgctt ctggtggcgc tcccctctct
      961 aaggaagtcg gggaagcggt tgccaagagg ttccatctgc caggtatcag gcaaggatat
     1021 gggctcactg agactacatc agctattctg attacacccg agggggatga taaaccgggc
     1081 gcggtcggta aagttgttcc attttttgaa gcgaaggttg tggatctgga taccgggaaa
     1141 acgctgggcg ttaatcaaag aggcgaactg tgtgtgagag gtcctatgat tatgtccggt
     1201 tatgtaaaca atccggaagc gaccaacgcc ttgattgaca aggatggatg gctacattct
     1261 ggagacatag cttactggga cgaagacgaa cacttcttca tcgttgaccg cctgaagtct
     1321 ctgattaagt acaaaggcta tcaggtggct cccgctgaat tggaatccat cttgctccaa
     1381 caccccaaca tcttcgacgc aggtgtcgca ggtcttcccg acgatgacgc cggtgaactt
     1441 cccgccgccg ttgttgtttt ggagcacgga aagacgatga cggaaaaaga gatcgtggat
     1501 tacgtcgcca gtcaagtaac aaccgcgaaa aagttgcgcg gaggagttgt gtttgtggac
     1561 gaagtaccga aaggtcttac cggaaaactc gacgcaagaa aaatcagaga gatcctcata
     1621 aaggccaaga agggcggaaa gatcgccgtg taatactaga gccaggcatc aaataaaacg
     1681 aaaggctcag tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct
     1741 ctactagagt cacactggct caccttcggg tgggcctttc tgcgtttata tactagagtt
     1801 tacagctagc tcagtcctag gtattatgct agctactaga gtcacacagg aaagtactag
     1861 atggcttcca aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg
     1921 tgggctcgct gcaagcaaat gaacgtgctg gactccttca tcaactacta tgattccgag
     1981 aagcacgccg agaacgccgt gatttttctg catggtaacg ctgcctccag ctacctgtgg
     2041 aggcacgtcg tgcctcacat cgagcccgtg gctagatgca tcatccctga tctgatcgga
     2101 atgggtaagt ccggcaagag cgggaatggc tcatatcgcc tcctggatca ctacaagtac
     2161 ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt gggccacgac
     2221 tggggggctt gtctggcctt tcactactcc tacgagcacc aagacaagat caaggccatc
     2281 gtccatgctg agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc tgacatcgag
     2341 gaggatatcg ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga gaataacttc
     2401 ttcgtcgaga ccatgctccc aagcaagatc atgcggaaac tggagcctga ggagttcgct
     2461 gcctacctgg agccattcaa ggagaagggc gaggttagac ggcctaccct ctcctggcct
     2521 cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg tccagattgt ccgcaactac
     2581 aacgcctacc ttcgggccag cgacgatctg cctaagatgt tcatcgagtc cgaccctggg
     2641 ttcttttcca acgctattgt cgagggagct aagaagttcc ctaacaccga gttcgtgaag
     2701 gtgaagggcc tccacttcag ccaggaggac gctccagatg aaatgggtaa gtacatcaag
     2761 agcttcgtgg agcgcgtgct gaagaacgag cagtaatact agagccaggc atcaaataaa
     2821 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc
     2881 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
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