LOCUS BBa_J95047 2933 bp DNA linear 26-Dec-2024 DEFINITION One of two RBS measurement kits B(B0015) ACCESSION BBa_J95047 VERSION BBa_J95047.1 FEATURES Location/Qualifiers misc_feature 1..936 /label=BBa_J95001 stem_loop 956..999 /label=stem_loop misc_feature 945..1024 /label=BBa_B0010 terminator 945..1024 /label=BBa_B0010 stem_loop 1040..1059 /label=T7 TE misc_feature 1066..1066 /label=stop polyA_site 1060..1073 /label=polya misc_feature 1033..1073 /label=BBa_B0012 terminator 1033..1073 /label=BBa_B0012 terminator 945..1073 /label=BBa_B0015 promoter 1082..1116 /label=BBa_J23101 RBS 1125..1137 /label=RBS-3\Weak RBS 1131..1134 /label=RBS misc_feature 1125..1137 /label=BBa_B0032 RBS 1125..1137 /label=BBa_B0032 misc_feature 1144..2796 /label=BBa_J95000 stem_loop 2816..2859 /label=stem_loop misc_feature 2805..2884 /label=BBa_B0010 terminator 2805..2884 /label=BBa_B0010 stem_loop 2900..2919 /label=T7 TE misc_feature 2926..2926 /label=stop polyA_site 2920..2933 /label=polya misc_feature 2893..2933 /label=BBa_B0012 terminator 2893..2933 /label=BBa_B0012 terminator 2805..2933 /label=BBa_B0015 ORIGIN 1 atggcttcca aggtgtacga ccccgagcaa cgcaaacgca tgatcactgg gcctcagtgg 61 tgggctcgct gcaagcaaat gaacgtgctg gactccttca tcaactacta tgattccgag 121 aagcacgccg agaacgccgt gatttttctg catggtaacg ctgcctccag ctacctgtgg 181 aggcacgtcg tgcctcacat cgagcccgtg gctagatgca tcatccctga tctgatcgga 241 atgggtaagt ccggcaagag cgggaatggc tcatatcgcc tcctggatca ctacaagtac 301 ctcaccgctt ggttcgagct gctgaacctt ccaaagaaaa tcatctttgt gggccacgac 361 tggggggctt gtctggcctt tcactactcc tacgagcacc aagacaagat caaggccatc 421 gtccatgctg agagtgtcgt ggacgtgatc gagtcctggg acgagtggcc tgacatcgag 481 gaggatatcg ccctgatcaa gagcgaagag ggcgagaaaa tggtgcttga gaataacttc 541 ttcgtcgaga ccatgctccc aagcaagatc atgcggaaac tggagcctga ggagttcgct 601 gcctacctgg agccattcaa ggagaagggc gaggttagac ggcctaccct ctcctggcct 661 cgcgagatcc ctctcgttaa gggaggcaag cccgacgtcg tccagattgt ccgcaactac 721 aacgcctacc ttcgggccag cgacgatctg cctaagatgt tcatcgagtc cgaccctggg 781 ttcttttcca acgctattgt cgagggagct aagaagttcc ctaacaccga gttcgtgaag 841 gtgaagggcc tccacttcag ccaggaggac gctccagatg aaatgggtaa gtacatcaag 901 agcttcgtgg agcgcgtgct gaagaacgag cagtaatact agagccaggc atcaaataaa 961 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 1021 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt atatactaga 1081 gtttacagct agctcagtcc taggtattat gctagctact agagtcacac aggaaagtac 1141 tagatggaag acgccaaaaa cataaagaaa ggcccggcgc cattctatcc gctggaagat 1201 ggaaccgctg gagagcaact gcataaggct atgaagagat acgccctggt tcctggaaca 1261 attgctttta cagatgcaca tatcgaggtg gacatcactt acgctgagta cttcgaaatg 1321 tccgttcggt tggcagaagc tatgaaacga tatgggctga atacaaatca cagaatcgtc 1381 gtatgcagtg aaaactctct tcaattcttt atgccggtgt tgggcgcgtt atttatcgga 1441 gttgcagttg cgcccgcgaa cgacatttat aatgaacgtg aattgctcaa cagtatgggc 1501 atttcgcagc ctaccgtggt gttcgtttcc aaaaaggggt tgcaaaaaat tttgaacgtg 1561 caaaaaaagc tcccaatcat ccaaaaaatt attatcatgg attctaaaac ggattaccag 1621 ggatttcagt cgatgtacac gttcgtcaca tctcatctac ctcccggttt taatgaatac 1681 gattttgtgc cagagtcctt cgatagggac aagacaattg cactgatcat gaactcctct 1741 ggatctactg gtctgcctaa aggtgtcgct ctgcctcata gaactgcctg cgtgagattc 1801 tcgcatgcca gagatcctat ttttggcaat caaatcattc cggatactgc gattttaagt 1861 gttgttccat tccatcacgg ttttggaatg tttactacac tcggatattt gatatgtgga 1921 tttcgagtcg tcttaatgta tagatttgaa gaagagctgt ttctgaggag ccttcaggat 1981 tacaagattc aaagtgcgct gctggtgcca accctattct ccttcttcgc caaaagcact 2041 ctgattgaca aatacgattt atctaattta cacgaaattg cttctggtgg cgctcccctc 2101 tctaaggaag tcggggaagc ggttgccaag aggttccatc tgccaggtat caggcaagga 2161 tatgggctca ctgagactac atcagctatt ctgattacac ccgaggggga tgataaaccg 2221 ggcgcggtcg gtaaagttgt tccatttttt gaagcgaagg ttgtggatct ggataccggg 2281 aaaacgctgg gcgttaatca aagaggcgaa ctgtgtgtga gaggtcctat gattatgtcc 2341 ggttatgtaa acaatccgga agcgaccaac gccttgattg acaaggatgg atggctacat 2401 tctggagaca tagcttactg ggacgaagac gaacacttct tcatcgttga ccgcctgaag 2461 tctctgatta agtacaaagg ctatcaggtg gctcccgctg aattggaatc catcttgctc 2521 caacacccca acatcttcga cgcaggtgtc gcaggtcttc ccgacgatga cgccggtgaa 2581 cttcccgccg ccgttgttgt tttggagcac ggaaagacga tgacggaaaa agagatcgtg 2641 gattacgtcg ccagtcaagt aacaaccgcg aaaaagttgc gcggaggagt tgtgtttgtg 2701 gacgaagtac cgaaaggtct taccggaaaa ctcgacgcaa gaaaaatcag agagatcctc 2761 ataaaggcca agaagggcgg aaagatcgcc gtgtaatact agagccaggc atcaaataaa 2821 acgaaaggct cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc 2881 tctctactag agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata //