BBa_J99008 1 BBa_J99008 CI857/pR thermoinducible promoter 2010-10-27T11:00:00Z 2015-05-08T01:08:32Z PCR amplified from pHK-11 Rossignol, M., Moulin, L., and Boccard, F. 2002. Phage hk022-based integrative vectors for the insertion of genes in the chromosome of multiply marked escherichia coli strains. FEMS Microbiology Letters 213:45-49. The thermosensitive CI857 mutant of the lambda phage CI repressor controls the expression of pR. In this system, pR is repressed at 30??C and activated at 42??C. false false _412_ 0 1481 412 Not in stock false na false David Bikard annotation2112470 1 pR range2112470 1 791 836 annotation2112473 1 pRM range2112473 1 755 790 annotation2112469 1 CI857 range2112469 1 39 752 annotation2112474 1 -10 range2112474 1 758 764 annotation2112471 1 -35 range2112471 1 800 804 annotation2112472 1 -10 range2112472 1 823 828 annotation2112475 1 -35 range2112475 1 780 788 BBa_J99008_sequence 1 ccggggatccatatgacgtcgacagaacaccttgccgatcagccaaacgtctcttcaggccactgactagcgataactttccccacaacggaacaactctcattgcatgggatcattgggtactgtgggtttagtggttgtaaaaacacctgaccgctatccctgatcagtttcttgaaggtaaactcatcacccccaagtctggctatgcagaaatcacctggctcaacagcctgctcagggtcaacgagaattaacattccgtcaggaaagcttggcttggagcctgttggtgcggtcatggaattaccttcaacctcaagccagaatgcagaatcactggcttttttggttgtgcttacccatctctccgcatcacctttggtaaaggttctaagcttaggtgagaacatccctgcctgaacatgagaaaaaacagggtactcatactcacttctaagtgacggctgcatactaaccgcttcatacatctcgtagatttctctggcgattgaagggctaaattcttcaacgctaactttgagaatttttgcaagcaatgcggcgttataagcatttaatgcattgatgccattaaataaagcaccaacgcctgactgccccatccccatcttgtctgcgacagattcctgggataagccaagttcatttttctttttttcataaattgctttaaggcgacgtgcgtcctcaagctgctcttgtgttaatggtttcttttttgtgctcatacgttaaatctatcaccgcaagggataaatatctaacaccgtgcgtgttgactattttacctctggcggtgataatggttgcatgtactaatcta igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 James Alastair McLaughlin Chris J. Myers 2017-03-06T15:00:00.000Z