LOCUS BBa_K076000 13176 bp DNA linear 26-Nov-2024 DEFINITION pLV-TRE-Ngn1-linker-EYFP-2A-mKate-Ubc-Bla ACCESSION BBa_K076000 VERSION BBa_K076000.1 FEATURES Location/Qualifiers misc_feature 1..10859 /label=BBa_K076001 CDS 10866..11597 /label=BBa_K076002 misc_feature 11606..11659 /label=BBa_K076003 misc_feature 11666..12382 /label=BBa_K076004 misc_feature 12391..12456 /label=BBa_K076005 misc_feature 12463..13176 /label=BBa_K076006 ORIGIN 1 taacgagttt actccctatc agtgatagag aacgtatgtc gagtttactc cctatcagtg 61 atagagaacg tatgtcgagt ttactcccta tcagtgatag agaacgatgt cgagtttact 121 ccctatcagt gatagagaac gtatgtcgag tttactccct atcagtgata gagaacgtat 181 gtcgagttta ctccctatca gtgatagaga acgtatgtcg agtttactcc ctatcagtga 241 tagagaacgt atgtcgaggt aggcgtgtac ggtgggaggc ctatataagc agagctcgtt 301 tagtgaaccg tcagatcgcc tgagttagaa ttcggccatt acggcctgcc accatgagca 361 gcccggatgc gggatacgcc agtgacgacc agagccagcc ccggagcgcg cagcccgcgg 421 tgatggcagg gttgggcccc tgtccctggg ccgagtccct gagccccctc ggggatgtaa 481 aggtgaaagg cgaggtggtg gcgagtagcg gggcgccagc cgggacgtcg ggccgagcca 541 aagcggagtc tcgcatccgg cggccgatga acgcctttat ggtgtgggcc aaagacgaac 601 gcaagcggtt ggcacagcag aacccagatc tgcacaacgc agagctaagc aagatgctag 661 gcaagtcttg gaaggcgttg accttggcag agaagcggcc cttcgtggaa gaggccgagc 721 ggctgcgcgt gcagcatatg caggaccacc ccaactacaa gtaccggccg cggcggcgca 781 agcaggtgaa gcgcatgaag cgggtggagg gaggcttcct gcacgctctc gtcgagcccc 841 aggccggcgc gcttggtccc gagggcggcc gcgtggccat ggatggcctg ggtctgcctt 901 tcccggagcc gggctatccg gccggtcctc cgctgatgtc tccgcacatg ggcccccact 961 atcgggactg ccagggactg ggcgctcccg cgctcgacgg ctaccctctg cccactccgg 1021 acacatcccc gctggatggc gtggagcagg acccggcttt ctttgcagcc ccgctgccag 1081 gggactgccc ggcggccggc acctacactt acgctccagt ctcggactat gcagtgtccg 1141 tagagccgcc cgctggcccc atgcgagtgg ggccggaccc ctcgggccct gcgatgccgg 1201 ggatcctggc gccccccagc gctctgcacc tgtactacgg cgcgatgggc tcgcccgccg 1261 caagtgcggg gcgcggtttc cacgcgcaac cccagcagcc gctgcaaccg caggcaccgc 1321 ccccgccacc gcagcagcag cacccagcgc acggccccgg gcaaccttcg ccccctcccg 1381 aggctctgcc ctgccgggat ggcacggaat ccaaccagcc cactgagctc ctaggggagg 1441 tggaccgcac ggaattcgaa cagtatctgc cctttgtgta taagcccgag atgggtcttc 1501 cctaccaggg acacgactgc ggagtgaacc tctcagacag ccacggagcc atttcctccg 1561 tggtgtccga cgctagctca gcggtctact attgcaacta ccccgacatt tgaggccgcc 1621 tcggccatta attaagtgat ctggcctccg cgccgggttt tggcgcctcc cgcgggcgcc 1681 cccctcctca cggcgagcgc tgccacgtca gacgaagggc gcaggagcgt cctgatcctt 1741 ccgcccggac gctcaggaca gcggcccgct gctcataaga ctcggcctta gaaccccagt 1801 atcagcagaa ggacatttta ggacgggact tgggtgactc tagggcactg gttttctttc 1861 cagagagcgg aacaggcgag gaaaagtagt cccttctcgg cgattctgcg gagggatctc 1921 cgtggggcgg tgaacgccga tgattatata aggacgcgcc gggtgtggca cagctagttc 1981 cgtcgcagcc gggatttggg tcgcggttct tgtttgtgga tcgctgtgat cgtcacttgg 2041 tgagtagcgg gctgctgggc tggccggggc tttcgtggcc gccgggccgc tcggtgggac 2101 ggaagcgtgt ggagagaccg ccaagggctg tagtctgggt ccgcgagcaa ggttgccctg 2161 aactgggggt tggggggagc gcagcaaaat ggcggctgtt cccgagtctt gaatggaaga 2221 cgcttgtgag gcgggctgtg aggtcgttga aacaaggtgg ggggcatggt gggcggcaag 2281 aacccaaggt cttgaggcct tcgctaatgc gggaaagctc ttattcgggt gagatgggct 2341 ggggcaccat ctggggaccc tgacgtgaag tttgtcactg actggagaac tcggtttgtc 2401 gtctgttgcg ggggcggcag ttatgcggtg ccgttgggca gtgcacccgt acctttggga 2461 gcgcgcgccc tcgtcgtgtc gtgacgtcac ccgttctgtt ggcttataat gcagggtggg 2521 gccacctgcc ggtaggtgtg cggtaggctt ttctccgtcg caggacgcag ggttcgggcc 2581 tagggtaggc tctcctgaat cgacaggcgc cggacctctg gtgaggggag ggataagtga 2641 ggcgtcagtt tctttggtcg gttttatgta cctatcttct taagtagctg aagctccggt 2701 tttgaactat gcgctcgggg ttggcgagtg tgttttgtga agttttttag gcaccttttg 2761 aaatgtaatc atttgggtca atatgtaatt ttcagtgtta gactagtaaa ttgtccgcta 2821 aattctggcc gtttttggct tttttgttag acgaagcgtg agctcgaggt cgactctaga 2881 tgccaccatg gccaagcctt tgtctcaaga agaatccacc ctcattgaaa gagcaacggc 2941 tacaatcaac agcatcccca tctctgaaga ctacagcgtc gccagcgcag ctctctctag 3001 cgacggccgc atcttcactg gtgtcaatgt atatcatttt actgggggac cttgtgcaga 3061 actcgtggtg ctgggcactg ctgctgctgc ggcagctggc aacctgactt gtatcgtcgc 3121 gatcggaaat gagaacaggg gcatcttgag cccctgcgga cggtgccgac aggtgcttct 3181 cgatctgcat cctgggatca aagccatagt gaaggacagt gatggacagc cgacggcagt 3241 tgggattcgt gaattgctgc cctctggtta tgtgtgggag ggctaagggc gcgccgttct 3301 agagaattcg atatcaagct tatcgataat caacctctgg attacaaaat ttgtgaaaga 3361 ttgactggta ttcttaacta tgttgctcct tttacgctat gtggatacgc tgctttaatg 3421 cctttgtatc atgctattac ttcccgtacg gctttcattt tctcctcctt gtataaatcc 3481 tggttgctgt ctctttatga ggagttgtgg cccgttgtca ggcaacgtgg cgtggtgtgc 3541 actgtgtttg ctgacgcaac ccccactggt tggggcattg ccaccaccta tcaactcctt 3601 tccgggactt tcgctttccc cctccctatt gccacggcgg aactcattgc cgcctgcctt 3661 gcccgctgct ggacaggggc tcggctgttg ggcactgaca attccgtggt gttgtcgggg 3721 aagctgacgt cctttccatg gctgctcgcc tgtgttgcca actggattct gcgcgggacg 3781 tccttctgct acgtcccttc ggccctcaat ccagcggacc ttccttcccg cggcctgctg 3841 ccggttctgc ggcctcttcc gcgtcttcgc cttcgccctc agacgagtcg gatctccctt 3901 tgggccgcct ccccgcctgc ctgcaggttt gtcgagacct agaaaaacat ggagcaatca 3961 caagtagcaa tacagcagct accaatgctg attgtgcctg gctagaagca caagaggagg 4021 aggaggtggg ttttccagtc acacctcagg tacctttaag accaatgact tacaaggcag 4081 ctgtagatct tagccacttt ttaaaagaaa aggggggact ggaagggcta attcactccc 4141 aacgaagaca agatctgctt tttgcttgta ctgggtctct ctggttagac cagatctgag 4201 cctgggagct ctctggctaa ctagggaacc cactgcttaa gcctcaataa agcttgcctt 4261 gagtgcttca agtagtgtgt gcccgtctgt tgtgtgactc tggtaactag agatccctca 4321 gaccctttta gtcagtgtgg aaaatctcta gcagggcccg tttaaacccg ctgatcagcc 4381 tcgactgtgc cttctagttg ccagccatct gttgtttgcc cctcccccgt gccttccttg 4441 accctggaag gtgccactcc cactgtcctt tcctaataaa atgaggaaat tgcatcgcat 4501 tgtctgagta ggtgtcattc tattctgggg ggtggggtgg ggcaggacag caagggggag 4561 gattgggaag acaatagcag gcatgctggg gatgcggtgg gctctatggc ttctgaggcg 4621 gaaagaacca gctggggctc tagggggtat ccccacgcgc cctgtagcgg cgcattaagc 4681 gcggcgggtg tggtggttac gcgcagcgtg accgctacac ttgccagcgc cctagcgccc 4741 gctcctttcg ctttcttccc ttcctttctc gccacgttcg ccggctttcc ccgtcaagct 4801 ctaaatcggg gcatcccttt agggttccga tttagtgctt tacggcacct cgaccccaaa 4861 aaacttgatt agggtgatgg ttcacgtagt gggccatcgc cctgatagac ggtttttcgc 4921 cctttgacgt tggagtccac gttctttaat agtggactct tgttccaaac tggaacaaca 4981 ctcaacccta tctcggtcta ttcttttgat ttataaggga ttttggggat ttcggcctat 5041 tggttaaaaa atgagctgat ttaacaaaaa tttaacgcga attaattctg tggaatgtgt 5101 gtcagttagg gtgtggaaag tccccaggct ccccaggcag gcagaagtat gcaaagcatg 5161 catctcaatt agtcagcaac caggtgtgga aagtccccag gctccccagc aggcagaagt 5221 atgcaaagca tgcatctcaa ttagtcagca accatagtcc cgcccctaac tccgcccatc 5281 ccgcccctaa ctccgcccag ttccgcccat tctccgcccc atggctgact aatttttttt 5341 atttatgcag aggccgaggc cgcctctgcc tctgagctat tccagaagta gtgaggaggc 5401 ttttttggag gcctaggctt ttgcaaaaag ctcccgggag cttgtatatc cattttcgga 5461 tctgatcagc acgtgttgac aattaatcat cggcatagta tatcggcata gtataatacg 5521 acaaggtgag gaactaaacc atggccaagt tgaccagtgc cgttccggtg ctcaccgcgc 5581 gcgacgtcgc cggagcggtc gagttctgga ccgaccggct cgggttctcc cgggacttcg 5641 tggaggacga cttcgccggt gtggtccggg acgacgtgac cctgttcatc agcgcggtcc 5701 aggaccaggt ggtgccggac aacaccctgg cctgggtgtg ggtgcgcggc ctggacgagc 5761 tgtacgccga gtggtcggag gtcgtgtcca cgaacttccg ggacgcctcc gggccggcca 5821 tgaccgagat cggcgagcag ccgtgggggc gggagttcgc cctgcgcgac ccggccggca 5881 actgcgtgca cttcgtggcc gaggagcagg actgacacgt gctacgagat ttcgattcca 5941 ccgccgcctt ctatgaaagg ttgggcttcg gaatcgtttt ccgggacgcc ggctggatga 6001 tcctccagcg cggggatctc atgctggagt tcttcgccca ccccaacttg tttattgcag 6061 cttataatgg ttacaaataa agcaatagca tcacaaattt cacaaataaa gcattttttt 6121 cactgcattc tagttgtggt ttgtccaaac tcatcaatgt atcttatcat gtctgtatac 6181 cgtcgacctc tagctagagc ttggcgtaat catggtcata gctgtttcct gtgtgaaatt 6241 gttatccgct cacaattcca cacaacatac gagccggaag cataaagtgt aaagcctggg 6301 gtgcctaatg agtgagctaa ctcacattaa ttgcgttgcg ctcactgccc gctttccagt 6361 cgggaaacct gtcgtgccag ctgcattaat gaatcggcca acgcgcgggg agaggcggtt 6421 tgcgtattgg gcgctcttcc gcttcctcgc tcactgactc gctgcgctcg gtcgttcggc 6481 tgcggcgagc ggtatcagct cactcaaagg cggtaatacg gttatccaca gaatcagggg 6541 ataacgcagg aaagaacatg tgagcaaaag gccagcaaaa ggccaggaac cgtaaaaagg 6601 ccgcgttgct ggcgtttttc cataggctcc gcccccctga cgagcatcac aaaaatcgac 6661 gctcaagtca gaggtggcga aacccgacag gactataaag ataccaggcg tttccccctg 6721 gaagctccct cgtgcgctct cctgttccga ccctgccgct taccggatac ctgtccgcct 6781 ttctcccttc gggaagcgtg gcgctttctc aatgctcacg ctgtaggtat ctcagttcgg 6841 tgtaggtcgt tcgctccaag ctgggctgtg tgcacgaacc ccccgttcag cccgaccgct 6901 gcgccttatc cggtaactat cgtcttgagt ccaacccggt aagacacgac ttatcgccac 6961 tggcagcagc cactggtaac aggattagca gagcgaggta tgtaggcggt gctacagagt 7021 tcttgaagtg gtggcctaac tacggctaca ctagaaggac agtatttggt atctgcgctc 7081 tgctgaagcc agttaccttc ggaaaaagag ttggtagctc ttgatccggc aaacaaacca 7141 ccgctggtag cggtggtttt tttgtttgca agcagcagat tacgcgcaga aaaaaaggat 7201 ctcaagaaga tcctttgatc ttttctacgg ggtctgacgc tcagtggaac gaaaactcac 7261 gttaagggat tttggtcatg agattatcaa aaaggatctt cacctagatc cttttaaatt 7321 aaaaatgaag ttttaaatca atctaaagta tatatgagta aacttggtct gacagttacc 7381 aatgcttaat cagtgaggca cctatctcag cgatctgtct atttcgttca tccatagttg 7441 cctgactccc cgtcgtgtag ataactacga tacgggaggg cttaccatct ggccccagtg 7501 ctgcaatgat accgcgagac ccacgctcac cggctccaga tttatcagca ataaaccagc 7561 cagccggaag ggccgagcgc agaagtggtc ctgcaacttt atccgcctcc atccagtcta 7621 ttaattgttg ccgggaagct agagtaagta gttcgccagt taatagtttg cgcaacgttg 7681 ttgccattgc tacaggcatc gtggtgtcac gctcgtcgtt tggtatggct tcattcagct 7741 ccggttccca acgatcaagg cgagttacat gatcccccat gttgtgcaaa aaagcggtta 7801 gctccttcgg tcctccgatc gttgtcagaa gtaagttggc cgcagtgtta tcactcatgg 7861 ttatggcagc actgcataat tctcttactg tcatgccatc cgtaagatgc ttttctgtga 7921 ctggtgagta ctcaaccaag tcattctgag aatagtgtat gcggcgaccg agttgctctt 7981 gcccggcgtc aatacgggat aataccgcgc cacatagcag aactttaaaa gtgctcatca 8041 ttggaaaacg ttcttcgggg cgaaaactct caaggatctt accgctgttg agatccagtt 8101 cgatgtaacc cactcgtgca cccaactgat cttcagcatc ttttactttc accagcgttt 8161 ctgggtgagc aaaaacagga aggcaaaatg ccgcaaaaaa gggaataagg gcgacacgga 8221 aatgttgaat actcatactc ttcctttttc aatattattg aagcatttat cagggttatt 8281 gtctcatgag cggatacata tttgaatgta tttagaaaaa taaacaaata ggggttccgc 8341 gcacatttcc ccgaaaagtg ccacctgacg tcgacggatc gggagatctc ccgatcccct 8401 atggtgcact ctcagtacaa tctgctctga tgccgcatag ttaagccagt atctgctccc 8461 tgcttgtgtg ttggaggtcg ctgagtagtg cgcgagcaaa atttaagcta caacaaggca 8521 aggcttgacc gacaattgca tgaagaatct gcttagggtt aggcgttttg cgctgcttcg 8581 cgatgtacgg gccagatata cgcgttgaca ttgattattg actagttatt aatagtaatc 8641 aattacgggg tcattagttc atagcccata tatggagttc cgcgttacat aacttacggt 8701 aaatggcccg cctggctgac cgcccaacga cccccgccca ttgacgtcaa taatgacgta 8761 tgttcccata gtaacgccaa tagggacttt ccattgacgt caatgggtgg actatttacg 8821 gtaaactgcc cacttggcag tacatcaagt gtatcatatg ccaagtacgc cccctattga 8881 cgtcaatgac ggtaaatggc ccgcctggca ttatgcccag tacatgacct tatgggactt 8941 tcctacttgg cagtacatct acgtattagt catcgctatt accatggtga tgcggttttg 9001 gcagtacatc aatgggcgtg gatagcggtt tgactcacgg ggatttccaa gtctccaccc 9061 cattgacgtc aatgggagtt tgttttggca ccaaaatcaa cgggactttc caaaatgtcg 9121 taacaactcc gccccattga cgcaaatggg cggtaggcgt gtacggtggg aggtctatat 9181 aagcagagct ctctggctaa ctagagaacc cactgcttac tggcttatcg aaattaatac 9241 gactcactat agggagaccc aagctggttt aaacttaagc ttggtaccga gctcactagt 9301 ccagtgtggt ggcagatatc cagcacagtg gcggccgctc gaggggcccg ttttgcctgt 9361 actgggtctc tctggttaga ccagatctga gcctgggagc tctctggcta actagggaac 9421 ccactgctta agcctcaata aagcttgcct tgagtgcttc aagtagtgtg tgcccgtctg 9481 ttgtgtgact ctggtaacta gagatccctc agaccctttt agtcagtgtg gaaaatctct 9541 agcagtggcg cccgaacagg gacttgaaag cgaaagggaa accagaggag ctctctcgac 9601 gcaggactcg gcttgctgaa gcgcgcacgg caagaggcga ggggcggcga ctggtgagta 9661 cgccaaaaat tttgactagc ggaggctaga aggagagaga tgggtgcgag agcgtcagta 9721 ttaagcgggg gagaattaga tcgcgatggg aaaaaattcg gttaaggcca gggggaaaga 9781 aaaaatataa attaaaacat atagtatggg caagcaggga gctagaacga ttcgcagtta 9841 atcctggcct gttagaaaca tcagaaggct gtagacaaat actgggacag ctacaaccat 9901 cccttcagac aggatcagaa gaacttagat cattatataa tacagtagca accctctatt 9961 gtgtgcatca aaggatagag ataaaagaca ccaaggaagc tttagacaag atagaggaag 10021 agcaaaacaa aagtaagacc accgcacagc aagcggccgc tgatcttcag acctggagga 10081 ggagatatga gggacaattg gagaagtgaa ttatataaat ataaagtagt aaaaattgaa 10141 ccattaggag tagcacccac caaggcaaag agaagagtgg tgcagagaga aaaaagagca 10201 gtgggaatag gagctttgtt ccttgggttc ttgggagcag caggaagcac tatgggcgca 10261 gcgtcaatga cgctgacggt acaggccaga caattattgt ctggtatagt gcagcagcag 10321 aacaatttgc tgagggctat tgaggcgcaa cagcatctgt tgcaactcac agtctggggc 10381 atcaagcagc tccaggcaag aatcctggct gtggaaagat acctaaagga tcaacagctc 10441 ctggggattt ggggttgctc tggaaaactc atttgcacca ctgctgtgcc ttggaatgct 10501 agttggagta ataaatctct ggaacagatt tggaatcaca cgacctggat ggagtgggac 10561 agagaaatta acaattacac aagcttaata cactccttaa ttgaagaatc gcaaaaccag 10621 caagaaaaga atgaacaaga attattggaa ttagataaat gggcaagttt gtggaattgg 10681 tttaacataa caaattggct gtggtatata aaattattca taatgatagt aggaggcttg 10741 gtaggtttaa gaatagtttt tgctgtactt tctatagtga atagagttag gcagggatat 10801 tcaccattat cgtttcagac ccacctccca accccgaggg gacccgacag gcccttaatt 10861 actagatgcc tgcccctttg gagacctgca tctctgatct cgactgctcc agcagcaaca 10921 gcagcagcga cctgtccagc ttcctcaccg acgaggagga ctgtgccagg ctacagcccc 10981 tagcctccac ctcggggctg tccgtgccag cccggaggag cgctcccgcc ctctccgggg 11041 catcgaatgt tcccggtgcc caggacgaag agcaggaacg gcggaggcgg cgaggtcgcg 11101 ctcgggtgcg gtccgaggct ctgctgcact ccctgcggag gagtcgtcgc gtcaaagcca 11161 acgatcgcga gcgcaaccgc atgcacaacc tcaacgctgc gctggacgcc ttgcgcagcg 11221 tgctgccctc gttccccgac gacaccaagc tcaccaagat tgagacgctg cgcttcgcct 11281 acaactacat ctgggccctg gctgagacac tgcgcctggc agatcaaggg ctccccgggg 11341 gcagtgcccg ggagcgcctc ctgcctccgc agtgtgtccc ctgtctgccc gggcccccga 11401 gcccggccag cgacactgag tcctggggtt ccggggccgc tgcctccccc tgcgccactg 11461 tggcatcacc actctctgac cccagtagtc cctcggcttc agaagacttc acctatggcc 11521 cgggcgatcc ccttttctcc tttcctggcc tgcccaaaga cctgctccac acgacgccct 11581 gtttcatccc ataccactac tagagggccg cctcggcccc ggcaacaacg gcggcaacaa 11641 caacggcggc tccaccggtt actagatggt gagcaagggc gaggagctgt tcaccggggt 11701 ggtgcccatc ctggtcgagc tggacggcga cgtaaacggc cacaagttca gcgtgtccgg 11761 cgagggcgag ggcgatgcca cctacggcaa gctgaccctg aagttcatct gcaccaccgg 11821 caagctgccc gtgccctggc ccaccctcgt gaccaccttc ggctacggcc tgcagtgctt 11881 cgcccgctac cccgaccaca tgaagcagca cgacttcttc aagtccgcca tgcccgaagg 11941 ctacgtccag gagcgcacca tcttcttcaa ggacgacggc aactacaaga cccgcgccga 12001 ggtgaagttc gagggcgaca ccctggtgaa ccgcatcgag ctgaagggca tcgacttcaa 12061 ggaggacggc aacatcctgg ggcacaagct ggagtacaac tacaacagcc acaacgtcta 12121 tatcatggcc gacaagcaga agaacggcat caaggtgaac ttcaagatcc gccacaacat 12181 cgaggacggc agcgtgcagc tcgccgacca ctaccagcag aacaccccca tcggcgacgg 12241 ccccgtgctg ctgcccgaca accactacct gagctaccag tccgccctga gcaaagaccc 12301 caacgagaag cgcgatcaca tggtcctgct ggagttcgtg accgccgccg ggatcactct 12361 cggcatggac gagctgtaca agtactagag gggtccggag ctacaaattt ttccctcctc 12421 aaacaggctg gagatgtcga agaaaatcct gggccttact agatggtgtc taagggcgaa 12481 gagctgatta aggagaacat gcacatgaag ctgtacatgg agggcaccgt gaacaaccac 12541 cacttcaagt gcacatccga gggcgaaggc aagccctacg agggcaccca gaccatgaga 12601 atcaaggtgg tcgagggcgg ccctctcccc ttcgccttcg acatcctggc taccagcttc 12661 atgtacggca gcaaaacctt catcaaccac acccagggca tccccgactt ctttaagcag 12721 tccttccctg agggcttcac atgggagaga gtcaccacat acgaagacgg gggcgtgctg 12781 accgctaccc aggacaccag cctccaggac ggctgcctca tctacaacgt caagatcaga 12841 ggggtgaact tcccatccaa cggccctgtg atgcagaaga aaacactcgg ctgggaggcc 12901 tccaccgaga tgctgtaccc cgctgacggc ggcctggaag gcagaagcga catggccctg 12961 aagctcgtgg gcgggggcca cctgatctgc aacttgaaga ccacatacag atccaagaaa 13021 cccgctaaga acctcaagat gcccggcgtc tactatgtgg acagaagact ggaaagaatc 13081 aaggaggccg acaaagagac ctacgtcgag cagcacgagg tggctgtggc cagatactgc 13141 gacctcccta gcaaactggg gcacaaactt aattaa //