LOCUS       BBa_K077002              995 bp    DNA     linear       14-May-2024
DEFINITION  pluxR with cI (lambda) behind a weak RBS
ACCESSION   BBa_K077002
VERSION     BBa_K077002.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    70..73
                     /label=conserved
     RBS             64..77
                     /label=BBa_B0031
     CDS             87..794
                     /label=cI lambda
     misc_feature    795..827
                     /label=LVA
     misc_feature    834..858
                     /label=Help:Barcodes
     CDS             84..833
                     /label=BBa_C0051
     stem_loop       878..921
                     /label=stem_loop
     misc_feature    867..946
                     /label=BBa_B0010
     terminator      867..946
                     /label=BBa_B0010
     stem_loop       962..981
                     /label=T7 TE
     misc_feature    988..988
                     /label=stop
     polyA_site      982..995
                     /label=polya
     misc_feature    955..995
                     /label=BBa_B0012
     terminator      955..995
                     /label=BBa_B0012
     misc_feature    64..995
                     /label=BBa_P0151
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagtcacaca ggaaacctac tagatgagca caaaaaagaa accattaaca caagagcagc
      121 ttgaggacgc acgtcgcctt aaagcaattt atgaaaaaaa gaaaaatgaa cttggcttat
      181 cccaggaatc tgtcgcagac aagatgggga tggggcagtc aggcgttggt gctttattta
      241 atggcatcaa tgcattaaat gcttataacg ccgcattgct tgcaaaaatt ctcaaagtta
      301 gcgttgaaga atttagccct tcaatcgcca gagaaatcta cgagatgtat gaagcggtta
      361 gtatgcagcc gtcacttaga agtgagtatg agtaccctgt tttttctcat gttcaggcag
      421 ggatgttctc acctgagctt agaaccttta ccaaaggtga tgcggagaga tgggtaagca
      481 caaccaaaaa agccagtgat tctgcattct ggcttgaggt tgaaggtaat tccatgaccg
      541 caccaacagg ctccaagcca agctttcctg acggaatgtt aattctcgtt gaccctgagc
      601 aggctgttga gccaggtgat ttctgcatag ccagacttgg gggtgatgag tttaccttca
      661 agaaactgat cagggatagc ggtcaggtgt ttttacaacc actaaaccca cagtacccaa
      721 tgatcccatg caatgagagt tgttccgttg tggggaaagt tatcgctagt cagtggcctg
      781 aagagacgtt tggcgctgca aacgacgaaa actacgcttt agtagcttaa taacgctgat
      841 agtgctagtg tagatcgcta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
      901 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
      961 tggctcacct tcgggtgggc ctttctgcgt ttata
//