LOCUS       BBa_K077003             1649 bp    DNA     linear       16-May-2024
DEFINITION  Hybrid promoter (cI and luxR regulated) and LuxI and GFP
ACCESSION   BBa_K077003
VERSION     BBa_K077003.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     misc_feature    703..734
                     /label=LVA
     CDS             124..702
                     /label=luxI
     misc_feature    124..741
                     /label=BBa_C0061
     misc_feature    742..766
                     /label=Help:Barcodes
     CDS             124..741
                     /label=BBa_C0061
     misc_feature    779..782
                     /label=conserved
     RBS             775..786
                     /label=BBa_B0034
     CDS             793..1512
                     /label=GFP protein
     CDS             793..1512
                     /label=BBa_E0040
     stem_loop       1532..1575
                     /label=stem_loop
     misc_feature    1521..1600
                     /label=BBa_B0010
     terminator      1521..1600
                     /label=BBa_B0010
     stem_loop       1616..1635
                     /label=T7 TE
     misc_feature    1642..1642
                     /label=stop
     polyA_site      1636..1649
                     /label=polya
     misc_feature    1609..1649
                     /label=BBa_B0012
     terminator      1609..1649
                     /label=BBa_B0012
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac
      121 tagatgacta taatgataaa aaaatcggat tttttggcaa ttccatcgga ggagtataaa
      181 ggtattctaa gtcttcgtta tcaagtgttt aagcaaagac ttgagtggga cttagttgta
      241 gaaaataacc ttgaatcaga tgagtatgat aactcaaatg cagaatatat ttatgcttgt
      301 gatgatactg aaaatgtaag tggatgctgg cgtttattac ctacaacagg tgattatatg
      361 ctgaaaagtg tttttcctga attgcttggt caacagagtg ctcccaaaga tcctaatata
      421 gtcgaattaa gtcgttttgc tgtaggtaaa aatagctcaa agataaataa ctctgctagt
      481 gaaattacaa tgaaactatt tgaagctata tataaacacg ctgttagtca aggtattaca
      541 gaatatgtaa cagtaacatc aacagcaata gagcgatttt taaagcgtat taaagttcct
      601 tgtcatcgta ttggagacaa agaaattcat gtattaggtg atactaaatc ggttgtattg
      661 tctatgccta ttaatgaaca gtttaaaaaa gcagtcttaa atgctgcaaa cgacgaaaac
      721 tacgctttag tagcttaata actctgatag tgctagtgta gatctctact agagaaagag
      781 gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt
      841 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat
      901 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca
      961 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat
     1021 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga
     1081 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt
     1141 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt
     1201 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa
     1261 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt
     1321 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca
     1381 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac
     1441 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta
     1501 tacaaataat aatactagag ccaggcatca aataaaacga aaggctcagt cgaaagactg
     1561 ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc acactggctc
     1621 accttcgggt gggcctttct gcgtttata
//