LOCUS       BBa_K077005             1752 bp    DNA     linear       14-May-2024
DEFINITION  LacI represseble promoter with LuxI and GFP behind
ACCESSION   BBa_K077005
VERSION     BBa_K077005.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     misc_feature    806..837
                     /label=LVA
     CDS             227..805
                     /label=luxI
     misc_feature    227..844
                     /label=BBa_C0061
     misc_feature    845..869
                     /label=Help:Barcodes
     CDS             227..844
                     /label=BBa_C0061
     misc_feature    882..885
                     /label=conserved
     RBS             878..889
                     /label=BBa_B0034
     CDS             896..1615
                     /label=GFP protein
     CDS             896..1615
                     /label=BBa_E0040
     stem_loop       1635..1678
                     /label=stem_loop
     misc_feature    1624..1703
                     /label=BBa_B0010
     terminator      1624..1703
                     /label=BBa_B0010
     stem_loop       1719..1738
                     /label=T7 TE
     misc_feature    1745..1745
                     /label=stop
     polyA_site      1739..1752
                     /label=polya
     misc_feature    1712..1752
                     /label=BBa_B0012
     terminator      1712..1752
                     /label=BBa_B0012
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga ctataatgat
      241 aaaaaaatcg gattttttgg caattccatc ggaggagtat aaaggtattc taagtcttcg
      301 ttatcaagtg tttaagcaaa gacttgagtg ggacttagtt gtagaaaata accttgaatc
      361 agatgagtat gataactcaa atgcagaata tatttatgct tgtgatgata ctgaaaatgt
      421 aagtggatgc tggcgtttat tacctacaac aggtgattat atgctgaaaa gtgtttttcc
      481 tgaattgctt ggtcaacaga gtgctcccaa agatcctaat atagtcgaat taagtcgttt
      541 tgctgtaggt aaaaatagct caaagataaa taactctgct agtgaaatta caatgaaact
      601 atttgaagct atatataaac acgctgttag tcaaggtatt acagaatatg taacagtaac
      661 atcaacagca atagagcgat ttttaaagcg tattaaagtt ccttgtcatc gtattggaga
      721 caaagaaatt catgtattag gtgatactaa atcggttgta ttgtctatgc ctattaatga
      781 acagtttaaa aaagcagtct taaatgctgc aaacgacgaa aactacgctt tagtagctta
      841 ataactctga tagtgctagt gtagatctct actagagaaa gaggagaaat actagatgcg
      901 taaaggagaa gaacttttca ctggagttgt cccaattctt gttgaattag atggtgatgt
      961 taatgggcac aaattttctg tcagtggaga gggtgaaggt gatgcaacat acggaaaact
     1021 tacccttaaa tttatttgca ctactggaaa actacctgtt ccatggccaa cacttgtcac
     1081 tactttcggt tatggtgttc aatgctttgc gagataccca gatcatatga aacagcatga
     1141 ctttttcaag agtgccatgc ccgaaggtta tgtacaggaa agaactatat ttttcaaaga
     1201 tgacgggaac tacaagacac gtgctgaagt caagtttgaa ggtgataccc ttgttaatag
     1261 aatcgagtta aaaggtattg attttaaaga agatggaaac attcttggac acaaattgga
     1321 atacaactat aactcacaca atgtatacat catggcagac aaacaaaaga atggaatcaa
     1381 agttaacttc aaaattagac acaacattga agatggaagc gttcaactag cagaccatta
     1441 tcaacaaaat actccaattg gcgatggccc tgtcctttta ccagacaacc attacctgtc
     1501 cacacaatct gccctttcga aagatcccaa cgaaaagaga gaccacatgg tccttcttga
     1561 gtttgtaaca gctgctggga ttacacatgg catggatgaa ctatacaaat aataatacta
     1621 gagccaggca tcaaataaaa cgaaaggctc agtcgaaaga ctgggccttt cgttttatct
     1681 gttgtttgtc ggtgaacgct ctctactaga gtcacactgg ctcaccttcg ggtgggcctt
     1741 tctgcgttta ta
//