LOCUS       BBa_K077007             1075 bp    DNA     linear       14-May-2024
DEFINITION  placI  and normal RBS with cII behind
ACCESSION   BBa_K077007
VERSION     BBa_K077007.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    213..216
                     /label=conserved
     RBS             209..220
                     /label=BBa_B0034
     CDS             227..874
                     /label=cII p22
     misc_feature    875..907
                     /label=LVA
     misc_feature    908..913
                     /label=stop
     misc_feature    227..913
                     /label=BBa_C0053
     misc_feature    914..938
                     /label=Help:Barcodes
     CDS             227..913
                     /label=BBa_C0053
     stem_loop       958..1001
                     /label=stem_loop
     misc_feature    947..1026
                     /label=BBa_B0010
     terminator      947..1026
                     /label=BBa_B0010
     stem_loop       1042..1061
                     /label=T7 TE
     misc_feature    1068..1068
                     /label=stop
     polyA_site      1062..1075
                     /label=polya
     misc_feature    1035..1075
                     /label=BBa_B0012
     terminator      1035..1075
                     /label=BBa_B0012
     misc_feature    209..1075
                     /label=BBa_P0453
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga atacacaatt
      241 gatgggtgag cgtattcgcg ctcgaagaaa aaaactcaag attagacaag ccgctcttgg
      301 taagatggtg ggagtgtcta atgttgcaat atcgcaatgg gagcgctcgg agactgagcc
      361 aaatggggag aacctgttgg cactttcgaa ggctcttcag tgctcccctg actatttgct
      421 gaaaggagat ttaagccaga caaacgttgc ctatcatagt aggcatgagc caagaggatc
      481 ataccctctt atcagttggg taagcgcagg gcaatggatg gaagctgtag aaccttatca
      541 caagcgcgcg atagagaact ggcacgacac cactgtagat tgttcagaag attcattttg
      601 gcttgatgtc caaggtgact ctatgacagc accggcaggg ttaagcattc cagaaggaat
      661 gataattctg gttgatcccg aagtcgaacc aagaaacggc aagctggttg ttgcaaaatt
      721 agaaggtgaa aacgaggcca cattcaaaaa attagttatg gatgcaggcc gaaagttttt
      781 aaaaccatta aacccacaat atccgatgat agaaatcaac ggaaactgca aaatcattgg
      841 cgtagttgtt gacgcaaaac tcgcaaatct tccagctgca aacgacgaaa actacgcttt
      901 agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata
      961 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac
     1021 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttata
//