##gff-version 3 ##sequence-region BBa_K077007 1 1075 BBa_K077007 . engineered_region 209 1075 . + 0 ID=BBa_P0453;Name=BBa_P0453 BBa_K077007 . terminator 947 1026 . + 0 ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_P0453 BBa_K077007 . engineered_region 947 1026 . + 0 ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010 BBa_K077007 . stem_loop 958 1001 . + 0 ID=annotation4184;Name=stem_loop;Parent=BBa_B0010 BBa_K077007 . ribosome_entry_site 209 220 . + 0 ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_P0453 BBa_K077007 . conserved_region 213 216 . + 0 ID=annotation23325;Name=conserved;Parent=BBa_B0034 BBa_K077007 . CDS 227 913 . + 0 ID=BBa_C0053;Name=BBa_C0053;Parent=BBa_P0453 BBa_K077007 . engineered_tag 914 938 . + 0 ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053 BBa_K077007 . engineered_region 227 913 . + 0 ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053 BBa_K077007 . tag 875 907 . + 0 ID=annotation1750;Name=LVA;Parent=BBa_C0053 BBa_K077007 . CDS 227 874 . + 0 ID=annotation1747;Name=cII p22;Parent=BBa_C0053 BBa_K077007 . stop_codon 908 913 . + 0 ID=annotation1751;Name=stop;Parent=BBa_C0053 BBa_K077007 . terminator 1035 1075 . + 0 ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_P0453 BBa_K077007 . polyA_site 1062 1075 . + 0 ID=annotation1690;Name=polya;Parent=BBa_B0012 BBa_K077007 . engineered_region 1035 1075 . + 0 ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012 BBa_K077007 . stop_codon 1068 1068 . + 0 ID=annotation1687;Name=stop;Parent=BBa_B0012 BBa_K077007 . stem_loop 1042 1061 . + 0 ID=annotation1686;Name=T7 TE;Parent=BBa_B0012 BBa_K077007 . promoter 1 200 . + 0 ID=BBa_R0010;Name=BBa_R0010 BBa_K077007 . non_covalent_binding_site 89 126 . + 0 ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010 BBa_K077007 . start_codon 173 173 . + 0 ID=annotation1961227;Name=start;Parent=BBa_R0010 BBa_K077007 . non_covalent_binding_site 166 200 . + 0 ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010 BBa_K077007 . engineered_region 1 200 . + 0 ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010 BBa_K077007 . promoter 161 166 . + 0 ID=annotation1961225;Name=-10;Parent=BBa_R0010 BBa_K077007 . CDS 1 88 . + 0 ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010 BBa_K077007 . promoter 137 142 . + 0 ID=annotation1961224;Name=-35;Parent=BBa_R0010 >BBa_K077007 caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaatacaca attgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtg tctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctc ttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaag aggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgata gagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcac cggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgt tgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccatta aacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatc ttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagcc aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct actagagtcacactggctcaccttcgggtgggcctttctgcgtttata