LOCUS       BBa_K077011             2148 bp    DNA     linear       26-Dec-2024
DEFINITION  LacI and AiiA
ACCESSION   BBa_K077011
VERSION     BBa_K077011.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    1108..1146
                     /label=LVA
     CDS             19..1146
                     /label=lacI-LVA
     misc_feature    19..1146
                     /label=BBa_C0012
     misc_feature    1147..1171
                     /label=Help:Barcodes
     CDS             19..1146
                     /label=BBa_C0012
     misc_feature    1..1171
                     /label=BBa_S0100
     misc_feature    1184..1187
                     /label=conserved
     RBS             1180..1191
                     /label=BBa_B0034
     misc_feature    1198..1200
                     /label=start
     misc_feature    1981..1986
                     /label=2
     misc_feature    1948..1980
                     /label=LVA
     CDS             1198..1947
                     /label=aiiA
     misc_feature    1198..1986
                     /label=BBa_C0060
     misc_feature    1987..2011
                     /label=Help:Barcodes
     CDS             1198..1986
                     /label=BBa_C0060
     stem_loop       2031..2074
                     /label=stem_loop
     misc_feature    2020..2099
                     /label=BBa_B0010
     terminator      2020..2099
                     /label=BBa_B0010
     stem_loop       2115..2134
                     /label=T7 TE
     misc_feature    2141..2141
                     /label=stop
     polyA_site      2135..2148
                     /label=polya
     misc_feature    2108..2148
                     /label=BBa_B0012
     terminator      2108..2148
                     /label=BBa_B0012
     misc_feature    1180..2148
                     /label=BBa_I0460
ORIGIN
        1 aaagaggaga aatactagat ggtgaatgtg aaaccagtaa cgttatacga tgtcgcagag
       61 tatgccggtg tctcttatca gaccgtttcc cgcgtggtga accaggccag ccacgtttct
      121 gcgaaaacgc gggaaaaagt ggaagcggcg atggcggagc tgaattacat tcccaaccgc
      181 gtggcacaac aactggcggg caaacagtcg ttgctgattg gcgttgccac ctccagtctg
      241 gccctgcacg cgccgtcgca aattgtcgcg gcgattaaat ctcgcgccga tcaactgggt
      301 gccagcgtgg tggtgtcgat ggtagaacga agcggcgtcg aagcctgtaa agcggcggtg
      361 cacaatcttc tcgcgcaacg cgtcagtggg ctgatcatta actatccgct ggatgaccag
      421 gatgccattg ctgtggaagc tgcctgcact aatgttccgg cgttatttct tgatgtctct
      481 gaccagacac ccatcaacag tattattttc tcccatgaag acggtacgcg actgggcgtg
      541 gagcatctgg tcgcattggg tcaccagcaa atcgcgctgt tagcgggccc attaagttct
      601 gtctcggcgc gtctgcgtct ggctggctgg cataaatatc tcactcgcaa tcaaattcag
      661 ccgatagcgg aacgggaagg cgactggagt gccatgtccg gttttcaaca aaccatgcaa
      721 atgctgaatg agggcatcgt tcccactgcg atgctggttg ccaacgatca gatggcgctg
      781 ggcgcaatgc gcgccattac cgagtccggg ctgcgcgttg gtgcggatat ctcggtagtg
      841 ggatacgacg ataccgaaga cagctcatgt tatatcccgc cgttaaccac catcaaacag
      901 gattttcgcc tgctggggca aaccagcgtg gaccgcttgc tgcaactctc tcagggccag
      961 gcggtgaagg gcaatcagct gttgcccgtc tcactggtga aaagaaaaac caccctggcg
     1021 cccaatacgc aaaccgcctc tccccgcgcg ttggccgatt cattaatgca gctggcacga
     1081 caggtttccc gactggaaag cgggcaggct gcaaacgacg aaaactacgc tttagtagct
     1141 taataactct gatagtgcta gtgtagatct ctactagaga aagaggagaa atactagatg
     1201 acagtaaaga agctttattt cgtcccagca ggtcgttgta tgttggatca ttcgtctgtt
     1261 aatagtacat taacaccagg agaattatta gacttaccgg tttggtgtta tcttttggag
     1321 actgaagaag gacctatttt agtagataca ggtatgccag aaagtgcagt taataatgaa
     1381 ggtcttttta acggtacatt tgtcgaaggg caggttttac cgaaaatgac tgaagaagat
     1441 agaatcgtga atattttaaa acgggttggt tatgagccgg aagaccttct ttatattatt
     1501 agttctcact tgcattttga tcatgcagga ggaaatggcg cttttataaa tacaccaatc
     1561 attgtacagc gtgctgaata tgaggcggcg cagcatagcg aagaatattt gaaagaatgt
     1621 atattgccga atttaaacta caaaatcatt gaaggtgatt atgaagtcgt accaggagtt
     1681 caattattgc atacaccagg ccatactcca gggcatcaat cgctattaat tgagacagaa
     1741 aaatccggtc ctgtattatt aacgattgat gcatcgtata cgaaagagaa ttttgaaaat
     1801 gaagtgccat ttgcgggatt tgattcagaa ttagctttat cttcaattaa acgtttaaaa
     1861 gaagtggtga tgaaagagaa gccgattgtt ttctttggac atgatataga gcaggaaagg
     1921 ggatgtaaag tgttccctga atatatagct gcaaacgacg aaaactacgc tttagtagct
     1981 taataacgct gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa
     2041 aggctcagtc gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct
     2101 actagagtca cactggctca ccttcgggtg ggcctttctg cgtttata
//