LOCUS       BBa_K077013             1764 bp    DNA     linear       09-May-2024
DEFINITION  cI (lambda) promoter with AiiA and cII
ACCESSION   BBa_K077013
VERSION     BBa_K077013.1  
FEATURES             Location/Qualifiers
     promoter        15..20
                     /label=-35
     promoter        25..41
                     /label=OR1
     promoter        1..17
                     /label=OR2
     promoter        38..43
                     /label=-10
     misc_feature    1..49
                     /label=BBa_R1051
     promoter        1..49
                     /label=BBa_R1051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     misc_feature    76..78
                     /label=start
     misc_feature    859..864
                     /label=2
     misc_feature    826..858
                     /label=LVA
     CDS             76..825
                     /label=aiiA
     misc_feature    76..864
                     /label=BBa_C0060
     misc_feature    865..889
                     /label=Help:Barcodes
     CDS             76..864
                     /label=BBa_C0060
     misc_feature    902..905
                     /label=conserved
     RBS             898..909
                     /label=BBa_B0034
     CDS             916..1563
                     /label=cII p22
     misc_feature    1564..1596
                     /label=LVA
     misc_feature    1597..1602
                     /label=stop
     misc_feature    916..1602
                     /label=BBa_C0053
     misc_feature    1603..1627
                     /label=Help:Barcodes
     CDS             916..1602
                     /label=BBa_C0053
     stem_loop       1647..1690
                     /label=stem_loop
     misc_feature    1636..1715
                     /label=BBa_B0010
     terminator      1636..1715
                     /label=BBa_B0010
     stem_loop       1731..1750
                     /label=T7 TE
     misc_feature    1757..1757
                     /label=stop
     polyA_site      1751..1764
                     /label=polya
     misc_feature    1724..1764
                     /label=BBa_B0012
     terminator      1724..1764
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgaca attttacctc tggcggtgat actggttgct actagagaaa
       61 gaggagaaat actagatgac agtaaagaag ctttatttcg tcccagcagg tcgttgtatg
      121 ttggatcatt cgtctgttaa tagtacatta acaccaggag aattattaga cttaccggtt
      181 tggtgttatc ttttggagac tgaagaagga cctattttag tagatacagg tatgccagaa
      241 agtgcagtta ataatgaagg tctttttaac ggtacatttg tcgaagggca ggttttaccg
      301 aaaatgactg aagaagatag aatcgtgaat attttaaaac gggttggtta tgagccggaa
      361 gaccttcttt atattattag ttctcacttg cattttgatc atgcaggagg aaatggcgct
      421 tttataaata caccaatcat tgtacagcgt gctgaatatg aggcggcgca gcatagcgaa
      481 gaatatttga aagaatgtat attgccgaat ttaaactaca aaatcattga aggtgattat
      541 gaagtcgtac caggagttca attattgcat acaccaggcc atactccagg gcatcaatcg
      601 ctattaattg agacagaaaa atccggtcct gtattattaa cgattgatgc atcgtatacg
      661 aaagagaatt ttgaaaatga agtgccattt gcgggatttg attcagaatt agctttatct
      721 tcaattaaac gtttaaaaga agtggtgatg aaagagaagc cgattgtttt ctttggacat
      781 gatatagagc aggaaagggg atgtaaagtg ttccctgaat atatagctgc aaacgacgaa
      841 aactacgctt tagtagctta ataacgctga tagtgctagt gtagatcgct actagagaaa
      901 gaggagaaat actagatgaa tacacaattg atgggtgagc gtattcgcgc tcgaagaaaa
      961 aaactcaaga ttagacaagc cgctcttggt aagatggtgg gagtgtctaa tgttgcaata
     1021 tcgcaatggg agcgctcgga gactgagcca aatggggaga acctgttggc actttcgaag
     1081 gctcttcagt gctcccctga ctatttgctg aaaggagatt taagccagac aaacgttgcc
     1141 tatcatagta ggcatgagcc aagaggatca taccctctta tcagttgggt aagcgcaggg
     1201 caatggatgg aagctgtaga accttatcac aagcgcgcga tagagaactg gcacgacacc
     1261 actgtagatt gttcagaaga ttcattttgg cttgatgtcc aaggtgactc tatgacagca
     1321 ccggcagggt taagcattcc agaaggaatg ataattctgg ttgatcccga agtcgaacca
     1381 agaaacggca agctggttgt tgcaaaatta gaaggtgaaa acgaggccac attcaaaaaa
     1441 ttagttatgg atgcaggccg aaagttttta aaaccattaa acccacaata tccgatgata
     1501 gaaatcaacg gaaactgcaa aatcattggc gtagttgttg acgcaaaact cgcaaatctt
     1561 ccagctgcaa acgacgaaaa ctacgcttta gtagcttaat aacactgata gtgctagtgt
     1621 agatcactac tagagccagg catcaaataa aacgaaaggc tcagtcgaaa gactgggcct
     1681 ttcgttttat ctgttgtttg tcggtgaacg ctctctacta gagtcacact ggctcacctt
     1741 cgggtgggcc tttctgcgtt tata
//