LOCUS       BBa_K077015             1805 bp    DNA     linear       16-May-2024
DEFINITION  ptet with LuxR and LasR
ACCESSION   BBa_K077015
VERSION     BBa_K077015.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..82
                     /label=prefix
     misc_feature    254..254
                     /label=T
     misc_feature    572..572
                     /label=A
     CDS             81..830
                     /label=luxR
     misc_feature    81..836
                     /label=BBa_C0062
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0062
     misc_feature    874..877
                     /label=conserved
     RBS             870..881
                     /label=BBa_B0034
     CDS             888..1604
                     /label=lasR
     misc_feature    1605..1637
                     /label=LVA
     misc_feature    1367..1367
                     /label=G
     misc_feature    1644..1668
                     /label=Help:Barcodes
     CDS             888..1643
                     /label=BBa_C0079
     stem_loop       1688..1731
                     /label=stem_loop
     misc_feature    1677..1756
                     /label=BBa_B0010
     terminator      1677..1756
                     /label=BBa_B0010
     stem_loop       1772..1791
                     /label=T7 TE
     misc_feature    1798..1798
                     /label=stop
     polyA_site      1792..1805
                     /label=polya
     misc_feature    1765..1805
                     /label=BBa_B0012
     terminator      1765..1805
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta
      121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta
      181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat
      241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta
      301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt
      361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga
      421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg
      481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg
      541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata
      601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg
      661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg
      721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta
      781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact
      841 gatagtgcta gtgtagatca ctactagaga aagaggagaa atactagatg gccttggttg
      901 acggttttct tgagctggaa cgctcaagtg gaaaattgga gtggagcgcc atcctccaga
      961 agatggcgag cgaccttgga ttctcgaaga tcctgttcgg cctgttgcct aaggacagcc
     1021 aggactacga gaacgccttc atcgtcggca actacccggc cgcctggcgc gagcattacg
     1081 accgggctgg ctacgcgcgg gtcgacccga cggtcagtca ctgtacccag agcgtactgc
     1141 cgattttctg ggaaccgtcc atctaccaga cgcgaaagca gcacgagttc ttcgaggaag
     1201 cctcggccgc cggcctggtg tatgggctga ccatgccgct gcatggtgct cgcggcgaac
     1261 tcggcgcgct gagcctcagc gtggaagcgg aaaaccgggc cgaggccaac cgtttcatag
     1321 agtcggtcct gccgaccctg tggatgctca aggactacgc actgcaaagc ggtgccggac
     1381 tggccttcga acatccggtc agcaaaccgg tggttctgac cagccgggag aaggaagtgt
     1441 tgcagtggtg cgccatcggc aagaccagtt gggagatatc ggttatctgc aactgctcgg
     1501 aagccaatgt gaacttccat atgggaaata ttcggcggaa gttcggtgtg acctcccgcc
     1561 gcgtagcggc cattatggcc gttaatttgg gtcttattac tctcgctgca aacgacgaaa
     1621 actacgcttt agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccag
     1681 gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt
     1741 gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt
     1801 ttata
//