LOCUS       BBa_K077018             1035 bp    DNA     linear       22-Nov-2024
DEFINITION  Hybrid promoter (cI and luxR regulated) and normal RBS cI lambda
ACCESSION   BBa_K077018
VERSION     BBa_K077018.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     CDS             127..834
                     /label=cI lambda
     misc_feature    835..867
                     /label=LVA
     misc_feature    874..898
                     /label=Help:Barcodes
     CDS             124..873
                     /label=BBa_C0051
     stem_loop       918..961
                     /label=stem_loop
     misc_feature    907..986
                     /label=BBa_B0010
     terminator      907..986
                     /label=BBa_B0010
     stem_loop       1002..1021
                     /label=T7 TE
     misc_feature    1028..1028
                     /label=stop
     polyA_site      1022..1035
                     /label=polya
     misc_feature    995..1035
                     /label=BBa_B0012
     terminator      995..1035
                     /label=BBa_B0012
     misc_feature    106..1035
                     /label=BBa_P0451
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac
      121 tagatgagca caaaaaagaa accattaaca caagagcagc ttgaggacgc acgtcgcctt
      181 aaagcaattt atgaaaaaaa gaaaaatgaa cttggcttat cccaggaatc tgtcgcagac
      241 aagatgggga tggggcagtc aggcgttggt gctttattta atggcatcaa tgcattaaat
      301 gcttataacg ccgcattgct tgcaaaaatt ctcaaagtta gcgttgaaga atttagccct
      361 tcaatcgcca gagaaatcta cgagatgtat gaagcggtta gtatgcagcc gtcacttaga
      421 agtgagtatg agtaccctgt tttttctcat gttcaggcag ggatgttctc acctgagctt
      481 agaaccttta ccaaaggtga tgcggagaga tgggtaagca caaccaaaaa agccagtgat
      541 tctgcattct ggcttgaggt tgaaggtaat tccatgaccg caccaacagg ctccaagcca
      601 agctttcctg acggaatgtt aattctcgtt gaccctgagc aggctgttga gccaggtgat
      661 ttctgcatag ccagacttgg gggtgatgag tttaccttca agaaactgat cagggatagc
      721 ggtcaggtgt ttttacaacc actaaaccca cagtacccaa tgatcccatg caatgagagt
      781 tgttccgttg tggggaaagt tatcgctagt cagtggcctg aagagacgtt tggcgctgca
      841 aacgacgaaa actacgcttt agtagcttaa taacgctgat agtgctagtg tagatcgcta
      901 ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta
      961 tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct tcgggtgggc
     1021 ctttctgcgt ttata
//