LOCUS       BBa_K077020              998 bp    DNA     linear       26-Dec-2024
DEFINITION  ptet with LasR
ACCESSION   BBa_K077020
VERSION     BBa_K077020.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..797
                     /label=lasR
     misc_feature    798..830
                     /label=LVA
     misc_feature    560..560
                     /label=G
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0079
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggccttgg ttgacggttt tcttgagctg gaacgctcaa
      121 gtggaaaatt ggagtggagc gccatcctcc agaagatggc gagcgacctt ggattctcga
      181 agatcctgtt cggcctgttg cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg
      241 gcaactaccc ggccgcctgg cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc
      301 cgacggtcag tcactgtacc cagagcgtac tgccgatttt ctgggaaccg tccatctacc
      361 agacgcgaaa gcagcacgag ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc
      421 tgaccatgcc gctgcatggt gctcgcggcg aactcggcgc gctgagcctc agcgtggaag
      481 cggaaaaccg ggccgaggcc aaccgtttca tagagtcggt cctgccgacc ctgtggatgc
      541 tcaaggacta cgcactgcaa agcggtgccg gactggcctt cgaacatccg gtcagcaaac
      601 cggtggttct gaccagccgg gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca
      661 gttgggagat atcggttatc tgcaactgct cggaagccaa tgtgaacttc catatgggaa
      721 atattcggcg gaagttcggt gtgacctccc gccgcgtagc ggccattatg gccgttaatt
      781 tgggtcttat tactctcgct gcaaacgacg aaaactacgc tttagtagct taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttata
//
LOCUS       BBa_J28009               998 bp    DNA     linear       26-Dec-2024
DEFINITION  Operon 5 (a)
ACCESSION   BBa_J28009
VERSION     BBa_J28009.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     CDS             81..797
                     /label=lasR
     misc_feature    798..830
                     /label=LVA
     misc_feature    560..560
                     /label=G
     misc_feature    837..861
                     /label=Help:Barcodes
     CDS             81..836
                     /label=BBa_C0079
     misc_feature    63..861
                     /label=BBa_S03156
     stem_loop       881..924
                     /label=stem_loop
     misc_feature    870..949
                     /label=BBa_B0010
     terminator      870..949
                     /label=BBa_B0010
     stem_loop       965..984
                     /label=T7 TE
     misc_feature    991..991
                     /label=stop
     polyA_site      985..998
                     /label=polya
     misc_feature    958..998
                     /label=BBa_B0012
     terminator      958..998
                     /label=BBa_B0012
     terminator      870..998
                     /label=BBa_B0015
     misc_feature    63..998
                     /label=BBa_S03547
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atggccttgg ttgacggttt tcttgagctg gaacgctcaa
      121 gtggaaaatt ggagtggagc gccatcctcc agaagatggc gagcgacctt ggattctcga
      181 agatcctgtt cggcctgttg cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg
      241 gcaactaccc ggccgcctgg cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc
      301 cgacggtcag tcactgtacc cagagcgtac tgccgatttt ctgggaaccg tccatctacc
      361 agacgcgaaa gcagcacgag ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc
      421 tgaccatgcc gctgcatggt gctcgcggcg aactcggcgc gctgagcctc agcgtggaag
      481 cggaaaaccg ggccgaggcc aaccgtttca tagagtcggt cctgccgacc ctgtggatgc
      541 tcaaggacta cgcactgcaa agcggtgccg gactggcctt cgaacatccg gtcagcaaac
      601 cggtggttct gaccagccgg gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca
      661 gttgggagat atcggttatc tgcaactgct cggaagccaa tgtgaacttc catatgggaa
      721 atattcggcg gaagttcggt gtgacctccc gccgcgtagc ggccattatg gccgttaatt
      781 tgggtcttat tactctcgct gcaaacgacg aaaactacgc tttagtagct taataacact
      841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc
      901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca
      961 cactggctca ccttcgggtg ggcctttctg cgtttata
//