##gff-version 3
##sequence-region BBa_K077024 1 1801
BBa_K077024	.	engineered_region	1	795	.	+	0	ID=BBa_E0241;Name=BBa_E0241
BBa_K077024	.	ribosome_entry_site	1	13	.	+	0	ID=BBa_B0032;Name=BBa_B0032;Parent=BBa_E0241
BBa_K077024	.	engineered_region	1	13	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K077024	.	ribosome_entry_site	7	10	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K077024	.	ribosome_entry_site	1	13	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K077024	.	terminator	748	795	.	+	0	ID=BBa_B0016;Name=BBa_B0016;Parent=BBa_E0241
BBa_K077024	.	CDS	20	739	.	+	0	ID=BBa_E0040;Name=BBa_E0040;Parent=BBa_E0241
BBa_K077024	.	CDS	20	739	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K077024	.	engineered_region	804	1801	.	+	0	ID=BBa_I13018;Name=BBa_I13018
BBa_K077024	.	promoter	804	857	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_I13018
BBa_K077024	.	promoter	846	851	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K077024	.	non_covalent_binding_site	829	847	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K077024	.	promoter	823	828	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K077024	.	engineered_region	804	857	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K077024	.	non_covalent_binding_site	804	822	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K077024	.	ribosome_entry_site	866	877	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_I13018
BBa_K077024	.	conserved_region	870	873	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K077024	.	terminator	1673	1752	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_I13018
BBa_K077024	.	stem_loop	1684	1727	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K077024	.	engineered_region	1673	1752	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K077024	.	CDS	884	1639	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_I13018
BBa_K077024	.	CDS	884	1633	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K077024	.	point_mutation	1375	1375	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K077024	.	point_mutation	1057	1057	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K077024	.	engineered_tag	1640	1664	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K077024	.	engineered_region	884	1639	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K077024	.	engineered_region	884	885	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K077024	.	terminator	1761	1801	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_I13018
BBa_K077024	.	polyA_site	1788	1801	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K077024	.	stem_loop	1768	1787	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K077024	.	stop_codon	1794	1794	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K077024	.	engineered_region	1761	1801	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
>BBa_K077024
tcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggt
gatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaattta
tttgcactactggaaaactacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgag
atacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatattt
ttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaa
aaggtattgattttaaagaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacat
catggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaacta
gcagaccattatcaacaaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaat
ctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacaca
tggcatggatgaactatacaaataataatactagagctagcataaccccttggggcctctaaacgggtcttgaggggtt
ttttgtactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaag
aggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaa
taatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcct
cattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatt
taataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgc
tgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcat
acggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatg
cgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaa
caacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaata
ttaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaa
gtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtag
atcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgt
cggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata