LOCUS       BBa_K077025             2608 bp    DNA     linear       14-Jun-2024
DEFINITION  HSL/LuxR or LasR promotor test system
ACCESSION   BBa_K077025
VERSION     BBa_K077025.1  
FEATURES             Location/Qualifiers
     RBS             1..13
                     /label=RBS-3\Weak
     RBS             7..10
                     /label=RBS
     misc_feature    1..13
                     /label=BBa_B0032
     RBS             1..13
                     /label=BBa_B0032
     CDS             20..739
                     /label=GFP protein
     CDS             20..739
                     /label=BBa_E0040
     terminator      748..795
                     /label=BBa_B0016
     misc_feature    804..822
                     /label=TetR 1
     misc_feature    804..857
                     /label=BBa_R0040
     promoter        823..828
                     /label=-35
     misc_feature    829..847
                     /label=TetR 2
     promoter        846..851
                     /label=-10
     promoter        804..857
                     /label=BBa_R0040
     misc_feature    870..873
                     /label=conserved
     RBS             866..877
                     /label=BBa_B0034
     misc_feature    884..885
                     /label=prefix
     misc_feature    1057..1057
                     /label=T
     misc_feature    1375..1375
                     /label=A
     CDS             884..1633
                     /label=luxR
     misc_feature    884..1639
                     /label=BBa_C0062
     misc_feature    1640..1664
                     /label=Help:Barcodes
     CDS             884..1639
                     /label=BBa_C0062
     misc_feature    1677..1680
                     /label=conserved
     RBS             1673..1684
                     /label=BBa_B0034
     CDS             1691..2407
                     /label=lasR
     misc_feature    2408..2440
                     /label=LVA
     misc_feature    2170..2170
                     /label=G
     misc_feature    2447..2471
                     /label=Help:Barcodes
     CDS             1691..2446
                     /label=BBa_C0079
     stem_loop       2491..2534
                     /label=stem_loop
     misc_feature    2480..2559
                     /label=BBa_B0010
     terminator      2480..2559
                     /label=BBa_B0010
     stem_loop       2575..2594
                     /label=T7 TE
     misc_feature    2601..2601
                     /label=stop
     polyA_site      2595..2608
                     /label=polya
     misc_feature    2568..2608
                     /label=BBa_B0012
     terminator      2568..2608
                     /label=BBa_B0012
ORIGIN
        1 tcacacagga aagtactaga tgcgtaaagg agaagaactt ttcactggag ttgtcccaat
       61 tcttgttgaa ttagatggtg atgttaatgg gcacaaattt tctgtcagtg gagagggtga
      121 aggtgatgca acatacggaa aacttaccct taaatttatt tgcactactg gaaaactacc
      181 tgttccatgg ccaacacttg tcactacttt cggttatggt gttcaatgct ttgcgagata
      241 cccagatcat atgaaacagc atgacttttt caagagtgcc atgcccgaag gttatgtaca
      301 ggaaagaact atatttttca aagatgacgg gaactacaag acacgtgctg aagtcaagtt
      361 tgaaggtgat acccttgtta atagaatcga gttaaaaggt attgatttta aagaagatgg
      421 aaacattctt ggacacaaat tggaatacaa ctataactca cacaatgtat acatcatggc
      481 agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt agacacaaca ttgaagatgg
      541 aagcgttcaa ctagcagacc attatcaaca aaatactcca attggcgatg gccctgtcct
      601 tttaccagac aaccattacc tgtccacaca atctgccctt tcgaaagatc ccaacgaaaa
      661 gagagaccac atggtccttc ttgagtttgt aacagctgct gggattacac atggcatgga
      721 tgaactatac aaataataat actagagcta gcataacccc ttggggcctc taaacgggtc
      781 ttgaggggtt ttttgtacta gagtccctat cagtgataga gattgacatc cctatcagtg
      841 atagagatac tgagcactac tagagaaaga ggagaaatac tagatgaaaa acataaatgc
      901 cgacgacaca tacagaataa ttaataaaat taaagcttgt agaagcaata atgatattaa
      961 tcaatgctta tctgatatga ctaaaatggt acattgtgaa tattatttac tcgcgatcat
     1021 ttatcctcat tctatggtta aatctgatat ttcaatccta gataattacc ctaaaaaatg
     1081 gaggcaatat tatgatgacg ctaatttaat aaaatatgat cctatagtag attattctaa
     1141 ctccaatcat tcaccaatta attggaatat atttgaaaac aatgctgtaa ataaaaaatc
     1201 tccaaatgta attaaagaag cgaaaacatc aggtcttatc actgggttta gtttccctat
     1261 tcatacggct aacaatggct tcggaatgct tagttttgca cattcagaaa aagacaacta
     1321 tatagatagt ttatttttac atgcgtgtat gaacatacca ttaattgttc cttctctagt
     1381 tgataattat cgaaaaataa atatagcaaa taataaatca aacaacgatt taaccaaaag
     1441 agaaaaagaa tgtttagcgt gggcatgcga aggaaaaagc tcttgggata tttcaaaaat
     1501 attaggttgc agtgagcgta ctgtcacttt ccatttaacc aatgcgcaaa tgaaactcaa
     1561 tacaacaaac cgctgccaaa gtatttctaa agcaatttta acaggagcaa ttgattgccc
     1621 atactttaaa aattaataac actgatagtg ctagtgtaga tcactactag agaaagagga
     1681 gaaatactag atggccttgg ttgacggttt tcttgagctg gaacgctcaa gtggaaaatt
     1741 ggagtggagc gccatcctcc agaagatggc gagcgacctt ggattctcga agatcctgtt
     1801 cggcctgttg cctaaggaca gccaggacta cgagaacgcc ttcatcgtcg gcaactaccc
     1861 ggccgcctgg cgcgagcatt acgaccgggc tggctacgcg cgggtcgacc cgacggtcag
     1921 tcactgtacc cagagcgtac tgccgatttt ctgggaaccg tccatctacc agacgcgaaa
     1981 gcagcacgag ttcttcgagg aagcctcggc cgccggcctg gtgtatgggc tgaccatgcc
     2041 gctgcatggt gctcgcggcg aactcggcgc gctgagcctc agcgtggaag cggaaaaccg
     2101 ggccgaggcc aaccgtttca tagagtcggt cctgccgacc ctgtggatgc tcaaggacta
     2161 cgcactgcaa agcggtgccg gactggcctt cgaacatccg gtcagcaaac cggtggttct
     2221 gaccagccgg gagaaggaag tgttgcagtg gtgcgccatc ggcaagacca gttgggagat
     2281 atcggttatc tgcaactgct cggaagccaa tgtgaacttc catatgggaa atattcggcg
     2341 gaagttcggt gtgacctccc gccgcgtagc ggccattatg gccgttaatt tgggtcttat
     2401 tactctcgct gcaaacgacg aaaactacgc tttagtagct taataacact gatagtgcta
     2461 gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc gaaagactgg
     2521 gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca cactggctca
     2581 ccttcgggtg ggcctttctg cgtttata
//