LOCUS BBa_K077027 3112 bp DNA linear 11-May-2024 DEFINITION Represillator under influence of HSL with cI, cII and lacI ACCESSION BBa_K077027 VERSION BBa_K077027.1 FEATURES Location/Qualifiers CDS 1..88 /label=end of LacI coding region (inactive) misc_feature 1..200 /label=BBa_R0010 misc_feature 89..126 /label=CAP binding site promoter 137..142 /label=-35 promoter 161..166 /label=-10 misc_feature 166..200 /label=LacI binding site misc_feature 173..173 /label=start promoter 1..200 /label=BBa_R0010 misc_feature 213..216 /label=conserved RBS 209..220 /label=BBa_B0034 CDS 227..874 /label=cII p22 misc_feature 875..907 /label=LVA misc_feature 908..913 /label=stop misc_feature 227..913 /label=BBa_C0053 misc_feature 914..938 /label=Help:Barcodes CDS 227..913 /label=BBa_C0053 stem_loop 958..1001 /label=stem_loop misc_feature 947..1026 /label=BBa_B0010 terminator 947..1026 /label=BBa_B0010 stem_loop 1042..1061 /label=T7 TE misc_feature 1068..1068 /label=stop polyA_site 1062..1075 /label=polya misc_feature 1035..1075 /label=BBa_B0012 terminator 1035..1075 /label=BBa_B0012 misc_feature 1084..1103 /label=LuxR/HSL promoter 1103..1108 /label=-35 promoter 1125..1130 /label=-10 misc_feature 1136..1136 /label=start misc_feature 1084..1138 /label=BBa_R0062 promoter 1084..1138 /label=BBa_R0062 misc_feature 1153..1156 /label=conserved RBS 1147..1160 /label=BBa_B0031 CDS 1170..1877 /label=cI lambda misc_feature 1878..1910 /label=LVA misc_feature 1917..1941 /label=Help:Barcodes CDS 1167..1916 /label=BBa_C0051 stem_loop 1961..2004 /label=stem_loop misc_feature 1950..2029 /label=BBa_B0010 terminator 1950..2029 /label=BBa_B0010 stem_loop 2045..2064 /label=T7 TE misc_feature 2071..2071 /label=stop polyA_site 2065..2078 /label=polya misc_feature 2038..2078 /label=BBa_B0012 terminator 2038..2078 /label=BBa_B0012 promoter 2101..2106 /label=-35 promoter 2111..2127 /label=OR1 promoter 2087..2103 /label=OR2 promoter 2124..2129 /label=-10 misc_feature 2087..2135 /label=BBa_R1051 promoter 2087..2135 /label=BBa_R1051 misc_feature 2148..2151 /label=conserved RBS 2144..2155 /label=BBa_B0034 misc_feature 2162..2164 /label=start misc_feature 2945..2950 /label=2 misc_feature 2912..2944 /label=LVA CDS 2162..2911 /label=aiiA misc_feature 2162..2950 /label=BBa_C0060 misc_feature 2951..2975 /label=Help:Barcodes CDS 2162..2950 /label=BBa_C0060 stem_loop 2995..3038 /label=stem_loop misc_feature 2984..3063 /label=BBa_B0010 terminator 2984..3063 /label=BBa_B0010 stem_loop 3079..3098 /label=T7 TE misc_feature 3105..3105 /label=stop polyA_site 3099..3112 /label=polya misc_feature 3072..3112 /label=BBa_B0012 terminator 3072..3112 /label=BBa_B0012 ORIGIN 1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca 61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc 121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga 181 gcggataaca atttcacaca tactagagaa agaggagaaa tactagatga atacacaatt 241 gatgggtgag cgtattcgcg ctcgaagaaa aaaactcaag attagacaag ccgctcttgg 301 taagatggtg ggagtgtcta atgttgcaat atcgcaatgg gagcgctcgg agactgagcc 361 aaatggggag aacctgttgg cactttcgaa ggctcttcag tgctcccctg actatttgct 421 gaaaggagat ttaagccaga caaacgttgc ctatcatagt aggcatgagc caagaggatc 481 ataccctctt atcagttggg taagcgcagg gcaatggatg gaagctgtag aaccttatca 541 caagcgcgcg atagagaact ggcacgacac cactgtagat tgttcagaag attcattttg 601 gcttgatgtc caaggtgact ctatgacagc accggcaggg ttaagcattc cagaaggaat 661 gataattctg gttgatcccg aagtcgaacc aagaaacggc aagctggttg ttgcaaaatt 721 agaaggtgaa aacgaggcca cattcaaaaa attagttatg gatgcaggcc gaaagttttt 781 aaaaccatta aacccacaat atccgatgat agaaatcaac ggaaactgca aaatcattgg 841 cgtagttgtt gacgcaaaac tcgcaaatct tccagctgca aacgacgaaa actacgcttt 901 agtagcttaa taacactgat agtgctagtg tagatcacta ctagagccag gcatcaaata 961 aaacgaaagg ctcagtcgaa agactgggcc tttcgtttta tctgttgttt gtcggtgaac 1021 gctctctact agagtcacac tggctcacct tcgggtgggc ctttctgcgt ttatatacta 1081 gagacctgta ggatcgtaca ggtttacgca agaaaatggt ttgttatagt cgaataaata 1141 ctagagtcac acaggaaacc tactagatga gcacaaaaaa gaaaccatta acacaagagc 1201 agcttgagga cgcacgtcgc cttaaagcaa tttatgaaaa aaagaaaaat gaacttggct 1261 tatcccagga atctgtcgca gacaagatgg ggatggggca gtcaggcgtt ggtgctttat 1321 ttaatggcat caatgcatta aatgcttata acgccgcatt gcttgcaaaa attctcaaag 1381 ttagcgttga agaatttagc ccttcaatcg ccagagaaat ctacgagatg tatgaagcgg 1441 ttagtatgca gccgtcactt agaagtgagt atgagtaccc tgttttttct catgttcagg 1501 cagggatgtt ctcacctgag cttagaacct ttaccaaagg tgatgcggag agatgggtaa 1561 gcacaaccaa aaaagccagt gattctgcat tctggcttga ggttgaaggt aattccatga 1621 ccgcaccaac aggctccaag ccaagctttc ctgacggaat gttaattctc gttgaccctg 1681 agcaggctgt tgagccaggt gatttctgca tagccagact tgggggtgat gagtttacct 1741 tcaagaaact gatcagggat agcggtcagg tgtttttaca accactaaac ccacagtacc 1801 caatgatccc atgcaatgag agttgttccg ttgtggggaa agttatcgct agtcagtggc 1861 ctgaagagac gtttggcgct gcaaacgacg aaaactacgc tttagtagct taataacgct 1921 gatagtgcta gtgtagatcg ctactagagc caggcatcaa ataaaacgaa aggctcagtc 1981 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 2041 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtaac accgtgcgtg 2101 ttgacaattt tacctctggc ggtgatactg gttgctacta gagaaagagg agaaatacta 2161 gatgacagta aagaagcttt atttcgtccc agcaggtcgt tgtatgttgg atcattcgtc 2221 tgttaatagt acattaacac caggagaatt attagactta ccggtttggt gttatctttt 2281 ggagactgaa gaaggaccta ttttagtaga tacaggtatg ccagaaagtg cagttaataa 2341 tgaaggtctt tttaacggta catttgtcga agggcaggtt ttaccgaaaa tgactgaaga 2401 agatagaatc gtgaatattt taaaacgggt tggttatgag ccggaagacc ttctttatat 2461 tattagttct cacttgcatt ttgatcatgc aggaggaaat ggcgctttta taaatacacc 2521 aatcattgta cagcgtgctg aatatgaggc ggcgcagcat agcgaagaat atttgaaaga 2581 atgtatattg ccgaatttaa actacaaaat cattgaaggt gattatgaag tcgtaccagg 2641 agttcaatta ttgcatacac caggccatac tccagggcat caatcgctat taattgagac 2701 agaaaaatcc ggtcctgtat tattaacgat tgatgcatcg tatacgaaag agaattttga 2761 aaatgaagtg ccatttgcgg gatttgattc agaattagct ttatcttcaa ttaaacgttt 2821 aaaagaagtg gtgatgaaag agaagccgat tgttttcttt ggacatgata tagagcagga 2881 aaggggatgt aaagtgttcc ctgaatatat agctgcaaac gacgaaaact acgctttagt 2941 agcttaataa cgctgatagt gctagtgtag atcgctacta gagccaggca tcaaataaaa 3001 cgaaaggctc agtcgaaaga ctgggccttt cgttttatct gttgtttgtc ggtgaacgct 3061 ctctactaga gtcacactgg ctcaccttcg ggtgggcctt tctgcgttta ta //