##gff-version 3
##sequence-region BBa_K077027 1 3112
BBa_K077027	.	promoter	1	200	.	+	0	ID=BBa_R0010;Name=BBa_R0010
BBa_K077027	.	start_codon	173	173	.	+	0	ID=annotation1961227;Name=start;Parent=BBa_R0010
BBa_K077027	.	non_covalent_binding_site	89	126	.	+	0	ID=annotation1961223;Name=CAP binding site;Parent=BBa_R0010
BBa_K077027	.	engineered_region	1	200	.	+	0	ID=annotation1961222;Name=BBa_R0010;Parent=BBa_R0010
BBa_K077027	.	promoter	161	166	.	+	0	ID=annotation1961225;Name=-10;Parent=BBa_R0010
BBa_K077027	.	non_covalent_binding_site	166	200	.	+	0	ID=annotation1961226;Name=LacI binding site;Parent=BBa_R0010
BBa_K077027	.	promoter	137	142	.	+	0	ID=annotation1961224;Name=-35;Parent=BBa_R0010
BBa_K077027	.	CDS	1	88	.	+	0	ID=annotation1961221;Name=end of LacI coding region (inactive);Parent=BBa_R0010
BBa_K077027	.	ribosome_entry_site	1147	1160	.	+	0	ID=BBa_B0031;Name=BBa_B0031
BBa_K077027	.	conserved_region	1153	1156	.	+	0	ID=annotation23316;Name=conserved;Parent=BBa_B0031
BBa_K077027	.	terminator	2984	3063	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K077027	.	stem_loop	2995	3038	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K077027	.	engineered_region	2984	3063	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K077027	.	ribosome_entry_site	209	220	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K077027	.	conserved_region	213	216	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K077027	.	terminator	2038	2078	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K077027	.	polyA_site	2065	2078	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K077027	.	stem_loop	2045	2064	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K077027	.	engineered_region	2038	2078	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K077027	.	stop_codon	2071	2071	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K077027	.	terminator	1950	2029	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K077027	.	stem_loop	1961	2004	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K077027	.	engineered_region	1950	2029	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K077027	.	terminator	3072	3112	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K077027	.	polyA_site	3099	3112	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K077027	.	stem_loop	3079	3098	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K077027	.	engineered_region	3072	3112	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K077027	.	stop_codon	3105	3105	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K077027	.	terminator	1035	1075	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K077027	.	polyA_site	1062	1075	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K077027	.	stem_loop	1042	1061	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K077027	.	engineered_region	1035	1075	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K077027	.	stop_codon	1068	1068	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K077027	.	ribosome_entry_site	2144	2155	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K077027	.	conserved_region	2148	2151	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K077027	.	promoter	2087	2135	.	+	0	ID=BBa_R1051;Name=BBa_R1051
BBa_K077027	.	promoter	2101	2106	.	+	0	ID=annotation2075;Name=-35;Parent=BBa_R1051
BBa_K077027	.	engineered_region	2087	2135	.	+	0	ID=annotation7074;Name=BBa_R1051;Parent=BBa_R1051
BBa_K077027	.	promoter	2111	2127	.	+	0	ID=annotation2076;Name=OR1;Parent=BBa_R1051
BBa_K077027	.	promoter	2087	2103	.	+	0	ID=annotation2077;Name=OR2;Parent=BBa_R1051
BBa_K077027	.	promoter	2124	2129	.	+	0	ID=annotation2078;Name=-10;Parent=BBa_R1051
BBa_K077027	.	promoter	1084	1138	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K077027	.	promoter	1103	1108	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K077027	.	non_covalent_binding_site	1084	1103	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K077027	.	promoter	1125	1130	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K077027	.	engineered_region	1084	1138	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K077027	.	start_codon	1136	1136	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K077027	.	terminator	947	1026	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K077027	.	stem_loop	958	1001	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K077027	.	engineered_region	947	1026	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K077027	.	CDS	227	913	.	+	0	ID=BBa_C0053;Name=BBa_C0053
BBa_K077027	.	stop_codon	908	913	.	+	0	ID=annotation1751;Name=stop;Parent=BBa_C0053
BBa_K077027	.	engineered_tag	914	938	.	+	0	ID=annotation2213993;Name=Help:Barcodes;Parent=BBa_C0053
BBa_K077027	.	CDS	227	874	.	+	0	ID=annotation1747;Name=cII p22;Parent=BBa_C0053
BBa_K077027	.	engineered_region	227	913	.	+	0	ID=annotation7036;Name=BBa_C0053;Parent=BBa_C0053
BBa_K077027	.	tag	875	907	.	+	0	ID=annotation1750;Name=LVA;Parent=BBa_C0053
BBa_K077027	.	CDS	2162	2950	.	+	0	ID=BBa_C0060;Name=BBa_C0060
BBa_K077027	.	engineered_tag	2951	2975	.	+	0	ID=annotation2213987;Name=Help:Barcodes;Parent=BBa_C0060
BBa_K077027	.	start_codon	2162	2164	.	+	0	ID=annotation1754;Name=start;Parent=BBa_C0060
BBa_K077027	.	CDS	2162	2911	.	+	0	ID=annotation1757;Name=aiiA;Parent=BBa_C0060
BBa_K077027	.	tag	2912	2944	.	+	0	ID=annotation1756;Name=LVA;Parent=BBa_C0060
BBa_K077027	.	stop_codon	2945	2950	.	+	0	ID=annotation1755;Name=2;Parent=BBa_C0060
BBa_K077027	.	engineered_region	2162	2950	.	+	0	ID=annotation7037;Name=BBa_C0060;Parent=BBa_C0060
BBa_K077027	.	CDS	1167	1916	.	+	0	ID=BBa_C0051;Name=BBa_C0051
BBa_K077027	.	CDS	1170	1877	.	+	0	ID=annotation23334;Name=cI lambda;Parent=BBa_C0051
BBa_K077027	.	tag	1878	1910	.	+	0	ID=annotation23335;Name=LVA;Parent=BBa_C0051
BBa_K077027	.	engineered_tag	1917	1941	.	+	0	ID=annotation2213991;Name=Help:Barcodes;Parent=BBa_C0051
>BBa_K077027
caatacgcaaaccgcctctccccgcgcgttggccgattcattaatgcagctggcacgacaggtttcccgactggaaagc
gggcagtgagcgcaacgcaattaatgtgagttagctcactcattaggcaccccaggctttacactttatgcttccggct
cgtatgttgtgtggaattgtgagcggataacaatttcacacatactagagaaagaggagaaatactagatgaatacaca
attgatgggtgagcgtattcgcgctcgaagaaaaaaactcaagattagacaagccgctcttggtaagatggtgggagtg
tctaatgttgcaatatcgcaatgggagcgctcggagactgagccaaatggggagaacctgttggcactttcgaaggctc
ttcagtgctcccctgactatttgctgaaaggagatttaagccagacaaacgttgcctatcatagtaggcatgagccaag
aggatcataccctcttatcagttgggtaagcgcagggcaatggatggaagctgtagaaccttatcacaagcgcgcgata
gagaactggcacgacaccactgtagattgttcagaagattcattttggcttgatgtccaaggtgactctatgacagcac
cggcagggttaagcattccagaaggaatgataattctggttgatcccgaagtcgaaccaagaaacggcaagctggttgt
tgcaaaattagaaggtgaaaacgaggccacattcaaaaaattagttatggatgcaggccgaaagtttttaaaaccatta
aacccacaatatccgatgatagaaatcaacggaaactgcaaaatcattggcgtagttgttgacgcaaaactcgcaaatc
ttccagctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcactactagagcc
aggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctct
actagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagacctgtaggatcgtacaggttta
cgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaacctactagatgagcacaaaaaagaaac
cattaacacaagagcagcttgaggacgcacgtcgccttaaagcaatttatgaaaaaaagaaaaatgaacttggcttatc
ccaggaatctgtcgcagacaagatggggatggggcagtcaggcgttggtgctttatttaatggcatcaatgcattaaat
gcttataacgccgcattgcttgcaaaaattctcaaagttagcgttgaagaatttagcccttcaatcgccagagaaatct
acgagatgtatgaagcggttagtatgcagccgtcacttagaagtgagtatgagtaccctgttttttctcatgttcaggc
agggatgttctcacctgagcttagaacctttaccaaaggtgatgcggagagatgggtaagcacaaccaaaaaagccagt
gattctgcattctggcttgaggttgaaggtaattccatgaccgcaccaacaggctccaagccaagctttcctgacggaa
tgttaattctcgttgaccctgagcaggctgttgagccaggtgatttctgcatagccagacttgggggtgatgagtttac
cttcaagaaactgatcagggatagcggtcaggtgtttttacaaccactaaacccacagtacccaatgatcccatgcaat
gagagttgttccgttgtggggaaagttatcgctagtcagtggcctgaagagacgtttggcgctgcaaacgacgaaaact
acgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggct
cagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcacctt
cgggtgggcctttctgcgtttatatactagagtaacaccgtgcgtgttgacaattttacctctggcggtgatactggtt
gctactagagaaagaggagaaatactagatgacagtaaagaagctttatttcgtcccagcaggtcgttgtatgttggat
cattcgtctgttaatagtacattaacaccaggagaattattagacttaccggtttggtgttatcttttggagactgaag
aaggacctattttagtagatacaggtatgccagaaagtgcagttaataatgaaggtctttttaacggtacatttgtcga
agggcaggttttaccgaaaatgactgaagaagatagaatcgtgaatattttaaaacgggttggttatgagccggaagac
cttctttatattattagttctcacttgcattttgatcatgcaggaggaaatggcgcttttataaatacaccaatcattg
tacagcgtgctgaatatgaggcggcgcagcatagcgaagaatatttgaaagaatgtatattgccgaatttaaactacaa
aatcattgaaggtgattatgaagtcgtaccaggagttcaattattgcatacaccaggccatactccagggcatcaatcg
ctattaattgagacagaaaaatccggtcctgtattattaacgattgatgcatcgtatacgaaagagaattttgaaaatg
aagtgccatttgcgggatttgattcagaattagctttatcttcaattaaacgtttaaaagaagtggtgatgaaagagaa
gccgattgttttctttggacatgatatagagcaggaaaggggatgtaaagtgttccctgaatatatagctgcaaacgac
gaaaactacgctttagtagcttaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacg
aaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggc
tcaccttcgggtgggcctttctgcgtttata