LOCUS       BBa_I0460                969 bp    DNA     linear       25-Nov-2024
DEFINITION  aiiA Device (B0034.C0060.B0015)
ACCESSION   BBa_I0460
VERSION     BBa_I0460.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..21
                     /label=start
     misc_feature    802..807
                     /label=2
     misc_feature    769..801
                     /label=LVA
     CDS             19..768
                     /label=aiiA
     misc_feature    19..807
                     /label=BBa_C0060
     misc_feature    808..832
                     /label=Help:Barcodes
     CDS             19..807
                     /label=BBa_C0060
     stem_loop       852..895
                     /label=stem_loop
     misc_feature    841..920
                     /label=BBa_B0010
     terminator      841..920
                     /label=BBa_B0010
     stem_loop       936..955
                     /label=T7 TE
     misc_feature    962..962
                     /label=stop
     polyA_site      956..969
                     /label=polya
     misc_feature    929..969
                     /label=BBa_B0012
     terminator      929..969
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gacagtaaag aagctttatt tcgtcccagc aggtcgttgt
       61 atgttggatc attcgtctgt taatagtaca ttaacaccag gagaattatt agacttaccg
      121 gtttggtgtt atcttttgga gactgaagaa ggacctattt tagtagatac aggtatgcca
      181 gaaagtgcag ttaataatga aggtcttttt aacggtacat ttgtcgaagg gcaggtttta
      241 ccgaaaatga ctgaagaaga tagaatcgtg aatattttaa aacgggttgg ttatgagccg
      301 gaagaccttc tttatattat tagttctcac ttgcattttg atcatgcagg aggaaatggc
      361 gcttttataa atacaccaat cattgtacag cgtgctgaat atgaggcggc gcagcatagc
      421 gaagaatatt tgaaagaatg tatattgccg aatttaaact acaaaatcat tgaaggtgat
      481 tatgaagtcg taccaggagt tcaattattg catacaccag gccatactcc agggcatcaa
      541 tcgctattaa ttgagacaga aaaatccggt cctgtattat taacgattga tgcatcgtat
      601 acgaaagaga attttgaaaa tgaagtgcca tttgcgggat ttgattcaga attagcttta
      661 tcttcaatta aacgtttaaa agaagtggtg atgaaagaga agccgattgt tttctttgga
      721 catgatatag agcaggaaag gggatgtaaa gtgttccctg aatatatagc tgcaaacgac
      781 gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc gctactagag
      841 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      901 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      961 gcgtttata
//
LOCUS       BBa_K077031              969 bp    DNA     linear       25-Nov-2024
DEFINITION  AiiA + term
ACCESSION   BBa_K077031
VERSION     BBa_K077031.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..21
                     /label=start
     misc_feature    802..807
                     /label=2
     misc_feature    769..801
                     /label=LVA
     CDS             19..768
                     /label=aiiA
     misc_feature    19..807
                     /label=BBa_C0060
     misc_feature    808..832
                     /label=Help:Barcodes
     CDS             19..807
                     /label=BBa_C0060
     stem_loop       852..895
                     /label=stem_loop
     misc_feature    841..920
                     /label=BBa_B0010
     terminator      841..920
                     /label=BBa_B0010
     stem_loop       936..955
                     /label=T7 TE
     misc_feature    962..962
                     /label=stop
     polyA_site      956..969
                     /label=polya
     misc_feature    929..969
                     /label=BBa_B0012
     terminator      929..969
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gacagtaaag aagctttatt tcgtcccagc aggtcgttgt
       61 atgttggatc attcgtctgt taatagtaca ttaacaccag gagaattatt agacttaccg
      121 gtttggtgtt atcttttgga gactgaagaa ggacctattt tagtagatac aggtatgcca
      181 gaaagtgcag ttaataatga aggtcttttt aacggtacat ttgtcgaagg gcaggtttta
      241 ccgaaaatga ctgaagaaga tagaatcgtg aatattttaa aacgggttgg ttatgagccg
      301 gaagaccttc tttatattat tagttctcac ttgcattttg atcatgcagg aggaaatggc
      361 gcttttataa atacaccaat cattgtacag cgtgctgaat atgaggcggc gcagcatagc
      421 gaagaatatt tgaaagaatg tatattgccg aatttaaact acaaaatcat tgaaggtgat
      481 tatgaagtcg taccaggagt tcaattattg catacaccag gccatactcc agggcatcaa
      541 tcgctattaa ttgagacaga aaaatccggt cctgtattat taacgattga tgcatcgtat
      601 acgaaagaga attttgaaaa tgaagtgcca tttgcgggat ttgattcaga attagcttta
      661 tcttcaatta aacgtttaaa agaagtggtg atgaaagaga agccgattgt tttctttgga
      721 catgatatag agcaggaaag gggatgtaaa gtgttccctg aatatatagc tgcaaacgac
      781 gaaaactacg ctttagtagc ttaataacgc tgatagtgct agtgtagatc gctactagag
      841 ccaggcatca aataaaacga aaggctcagt cgaaagactg ggcctttcgt tttatctgtt
      901 gtttgtcggt gaacgctctc tactagagtc acactggctc accttcgggt gggcctttct
      961 gcgtttata
//