LOCUS       BBa_K077036             1906 bp    DNA     linear       22-Dec-2024
DEFINITION  testing hybrid LuxR/HSL/cI promotor output GFP
ACCESSION   BBa_K077036
VERSION     BBa_K077036.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     RBS             106..118
                     /label=RBS-3\Weak
     RBS             112..115
                     /label=RBS
     misc_feature    106..118
                     /label=BBa_B0032
     RBS             106..118
                     /label=BBa_B0032
     CDS             125..844
                     /label=GFP protein
     CDS             125..844
                     /label=BBa_E0040
     terminator      853..900
                     /label=BBa_B0016
     misc_feature    909..927
                     /label=TetR 1
     misc_feature    909..962
                     /label=BBa_R0040
     promoter        928..933
                     /label=-35
     misc_feature    934..952
                     /label=TetR 2
     promoter        951..956
                     /label=-10
     promoter        909..962
                     /label=BBa_R0040
     misc_feature    975..978
                     /label=conserved
     RBS             971..982
                     /label=BBa_B0034
     misc_feature    989..990
                     /label=prefix
     misc_feature    1162..1162
                     /label=T
     misc_feature    1480..1480
                     /label=A
     CDS             989..1738
                     /label=luxR
     misc_feature    989..1744
                     /label=BBa_C0062
     misc_feature    1745..1769
                     /label=Help:Barcodes
     CDS             989..1744
                     /label=BBa_C0062
     stem_loop       1789..1832
                     /label=stem_loop
     misc_feature    1778..1857
                     /label=BBa_B0010
     terminator      1778..1857
                     /label=BBa_B0010
     stem_loop       1873..1892
                     /label=T7 TE
     misc_feature    1899..1899
                     /label=stop
     polyA_site      1893..1906
                     /label=polya
     misc_feature    1866..1906
                     /label=BBa_B0012
     terminator      1866..1906
                     /label=BBa_B0012
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagtcaca caggaaagta
      121 ctagatgcgt aaaggagaag aacttttcac tggagttgtc ccaattcttg ttgaattaga
      181 tggtgatgtt aatgggcaca aattttctgt cagtggagag ggtgaaggtg atgcaacata
      241 cggaaaactt acccttaaat ttatttgcac tactggaaaa ctacctgttc catggccaac
      301 acttgtcact actttcggtt atggtgttca atgctttgcg agatacccag atcatatgaa
      361 acagcatgac tttttcaaga gtgccatgcc cgaaggttat gtacaggaaa gaactatatt
      421 tttcaaagat gacgggaact acaagacacg tgctgaagtc aagtttgaag gtgataccct
      481 tgttaataga atcgagttaa aaggtattga ttttaaagaa gatggaaaca ttcttggaca
      541 caaattggaa tacaactata actcacacaa tgtatacatc atggcagaca aacaaaagaa
      601 tggaatcaaa gttaacttca aaattagaca caacattgaa gatggaagcg ttcaactagc
      661 agaccattat caacaaaata ctccaattgg cgatggccct gtccttttac cagacaacca
      721 ttacctgtcc acacaatctg ccctttcgaa agatcccaac gaaaagagag accacatggt
      781 ccttcttgag tttgtaacag ctgctgggat tacacatggc atggatgaac tatacaaata
      841 ataatactag agctagcata accccttggg gcctctaaac gggtcttgag gggttttttg
      901 tactagagtc cctatcagtg atagagattg acatccctat cagtgataga gatactgagc
      961 actactagag aaagaggaga aatactagat gaaaaacata aatgccgacg acacatacag
     1021 aataattaat aaaattaaag cttgtagaag caataatgat attaatcaat gcttatctga
     1081 tatgactaaa atggtacatt gtgaatatta tttactcgcg atcatttatc ctcattctat
     1141 ggttaaatct gatatttcaa tcctagataa ttaccctaaa aaatggaggc aatattatga
     1201 tgacgctaat ttaataaaat atgatcctat agtagattat tctaactcca atcattcacc
     1261 aattaattgg aatatatttg aaaacaatgc tgtaaataaa aaatctccaa atgtaattaa
     1321 agaagcgaaa acatcaggtc ttatcactgg gtttagtttc cctattcata cggctaacaa
     1381 tggcttcgga atgcttagtt ttgcacattc agaaaaagac aactatatag atagtttatt
     1441 tttacatgcg tgtatgaaca taccattaat tgttccttct ctagttgata attatcgaaa
     1501 aataaatata gcaaataata aatcaaacaa cgatttaacc aaaagagaaa aagaatgttt
     1561 agcgtgggca tgcgaaggaa aaagctcttg ggatatttca aaaatattag gttgcagtga
     1621 gcgtactgtc actttccatt taaccaatgc gcaaatgaaa ctcaatacaa caaaccgctg
     1681 ccaaagtatt tctaaagcaa ttttaacagg agcaattgat tgcccatact ttaaaaatta
     1741 ataacactga tagtgctagt gtagatcact actagagcca ggcatcaaat aaaacgaaag
     1801 gctcagtcga aagactgggc ctttcgtttt atctgttgtt tgtcggtgaa cgctctctac
     1861 tagagtcaca ctggctcacc ttcgggtggg cctttctgcg tttata
//