##gff-version 3
##sequence-region BBa_K077036 1 1906
BBa_K077036	.	promoter	1	97	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_K077036	.	promoter	71	76	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_K077036	.	promoter	58	58	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_K077036	.	non_covalent_binding_site	57	73	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_K077036	.	promoter	94	97	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_K077036	.	non_covalent_binding_site	81	97	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_K077036	.	promoter	47	52	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_K077036	.	non_covalent_binding_site	6	25	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_K077036	.	engineered_region	1	97	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_K077036	.	terminator	1778	1857	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K077036	.	stem_loop	1789	1832	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K077036	.	engineered_region	1778	1857	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K077036	.	ribosome_entry_site	971	982	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K077036	.	conserved_region	975	978	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K077036	.	terminator	853	900	.	+	0	ID=BBa_B0016;Name=BBa_B0016
BBa_K077036	.	ribosome_entry_site	106	118	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K077036	.	engineered_region	106	118	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K077036	.	ribosome_entry_site	106	118	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K077036	.	ribosome_entry_site	112	115	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K077036	.	terminator	1866	1906	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K077036	.	polyA_site	1893	1906	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K077036	.	stem_loop	1873	1892	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K077036	.	engineered_region	1866	1906	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K077036	.	stop_codon	1899	1899	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K077036	.	promoter	909	962	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K077036	.	promoter	928	933	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K077036	.	promoter	951	956	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K077036	.	non_covalent_binding_site	909	927	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K077036	.	engineered_region	909	962	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K077036	.	non_covalent_binding_site	934	952	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K077036	.	CDS	989	1744	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K077036	.	engineered_region	989	1744	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K077036	.	engineered_tag	1745	1769	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K077036	.	CDS	989	1738	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K077036	.	point_mutation	1162	1162	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K077036	.	engineered_region	989	990	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K077036	.	point_mutation	1480	1480	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K077036	.	CDS	125	844	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K077036	.	CDS	125	844	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
>BBa_K077036
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactatt
ttacctctggcggtgatatactagagtcacacaggaaagtactagatgcgtaaaggagaagaacttttcactggagttg
tcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagagggtgaaggtgatgcaac
atacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaacacttgtcactactttc
ggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaagagtgccatgcccgaag
gttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagtcaagtttgaaggtga
tacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttggacacaaattggaatac
aactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaacttcaaaattagacaca
acattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatggccctgtccttttacc
agacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccacatggtccttcttgag
tttgtaacagctgctgggattacacatggcatggatgaactatacaaataataatactagagctagcataaccccttgg
ggcctctaaacgggtcttgaggggttttttgtactagagtccctatcagtgatagagattgacatccctatcagtgata
gagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataatt
aataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaat
attatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatg
gaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaatt
aattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtctta
tcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaa
ctatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaa
ataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaa
aaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaa
actcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaat
taataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactg
ggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttc
tgcgtttata