LOCUS       BBa_K077037             1645 bp    DNA     linear       26-Dec-2024
DEFINITION  Testing hybrid LuxR/HSL/cI promotor, output LacZ
ACCESSION   BBa_K077037
VERSION     BBa_K077037.1  
FEATURES             Location/Qualifiers
     misc_feature    1..97
                     /label=BBa_R0065
     misc_feature    6..25
                     /label=Lux Box
     promoter        47..52
                     /label=-10
     misc_feature    57..73
                     /label=OR2 cI
     promoter        58..58
                     /label=lux p(R) start
     promoter        71..76
                     /label=-35
     misc_feature    81..97
                     /label=OR1 cI
     promoter        94..97
                     /label=-10
     promoter        1..97
                     /label=BBa_R0065
     misc_feature    110..113
                     /label=conserved
     RBS             106..117
                     /label=BBa_B0034
     misc_feature    124..138
                     /label=lacZ gene fragment
     CDS             124..477
                     /label=lacZ alpha
     promoter        149..158
                     /label=T3 promoter
     promoter        296..314
                     /label=T7 promoter
     misc_feature    201..201
                     /label=C
     misc_feature    204..204
                     /label=T
     misc_feature    210..210
                     /label=A
     misc_feature    231..231
                     /label=C
     misc_feature    234..234
                     /label=G
     misc_feature    322..471
                     /label=lacZ gene fragment
     misc_feature    471..471
                     /label=inserted T
     misc_feature    478..502
                     /label=barcode
     misc_feature    124..502
                     /label=BBa_E0035
     stem_loop       522..565
                     /label=stem_loop
     misc_feature    511..590
                     /label=BBa_B0010
     terminator      511..590
                     /label=BBa_B0010
     stem_loop       606..625
                     /label=T7 TE
     misc_feature    632..632
                     /label=stop
     polyA_site      626..639
                     /label=polya
     misc_feature    599..639
                     /label=BBa_B0012
     terminator      599..639
                     /label=BBa_B0012
     terminator      511..639
                     /label=BBa_B0015
     misc_feature    106..639
                     /label=BBa_E0435
     misc_feature    648..666
                     /label=TetR 1
     misc_feature    648..701
                     /label=BBa_R0040
     promoter        667..672
                     /label=-35
     misc_feature    673..691
                     /label=TetR 2
     promoter        690..695
                     /label=-10
     promoter        648..701
                     /label=BBa_R0040
     misc_feature    714..717
                     /label=conserved
     RBS             710..721
                     /label=BBa_B0034
     misc_feature    728..729
                     /label=prefix
     misc_feature    901..901
                     /label=T
     misc_feature    1219..1219
                     /label=A
     CDS             728..1477
                     /label=luxR
     misc_feature    728..1483
                     /label=BBa_C0062
     misc_feature    1484..1508
                     /label=Help:Barcodes
     CDS             728..1483
                     /label=BBa_C0062
     stem_loop       1528..1571
                     /label=stem_loop
     misc_feature    1517..1596
                     /label=BBa_B0010
     terminator      1517..1596
                     /label=BBa_B0010
     stem_loop       1612..1631
                     /label=T7 TE
     misc_feature    1638..1638
                     /label=stop
     polyA_site      1632..1645
                     /label=polya
     misc_feature    1605..1645
                     /label=BBa_B0012
     terminator      1605..1645
                     /label=BBa_B0012
     misc_feature    648..1645
                     /label=BBa_S03119
     misc_feature    106..1645
                     /label=BBa_K077124
ORIGIN
        1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac
       61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac
      121 tagatgacca tgattacgcc aagcgcgcaa ttaaccctca ctaaagggaa caaaagctgg
      181 agctccaccg cggtggcggc agcactagag ctagtggatc ccccgggctg tagaaattcg
      241 atatcaagct tatcgatacc gtcgacctcg agggggggcc cggtacccaa ttcgccctat
      301 agtgagtcgt attacgcgcg ctcactggcc gtcgttttac aacgtcgtga ctgggaaaac
      361 cctggcgtta cccaacttaa tcgccttgca gcacatcccc ctttcgccag ctggcgtaat
      421 agcgaagagg cccgcaccga tcgcccttcc caacagttgc gcagcctgaa ttaataacgc
      481 tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga aaggctcagt
      541 cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc tactagagtc
      601 acactggctc accttcgggt gggcctttct gcgtttatat actagagtcc ctatcagtga
      661 tagagattga catccctatc agtgatagag atactgagca ctactagaga aagaggagaa
      721 atactagatg aaaaacataa atgccgacga cacatacaga ataattaata aaattaaagc
      781 ttgtagaagc aataatgata ttaatcaatg cttatctgat atgactaaaa tggtacattg
      841 tgaatattat ttactcgcga tcatttatcc tcattctatg gttaaatctg atatttcaat
      901 cctagataat taccctaaaa aatggaggca atattatgat gacgctaatt taataaaata
      961 tgatcctata gtagattatt ctaactccaa tcattcacca attaattgga atatatttga
     1021 aaacaatgct gtaaataaaa aatctccaaa tgtaattaaa gaagcgaaaa catcaggtct
     1081 tatcactggg tttagtttcc ctattcatac ggctaacaat ggcttcggaa tgcttagttt
     1141 tgcacattca gaaaaagaca actatataga tagtttattt ttacatgcgt gtatgaacat
     1201 accattaatt gttccttctc tagttgataa ttatcgaaaa ataaatatag caaataataa
     1261 atcaaacaac gatttaacca aaagagaaaa agaatgttta gcgtgggcat gcgaaggaaa
     1321 aagctcttgg gatatttcaa aaatattagg ttgcagtgag cgtactgtca ctttccattt
     1381 aaccaatgcg caaatgaaac tcaatacaac aaaccgctgc caaagtattt ctaaagcaat
     1441 tttaacagga gcaattgatt gcccatactt taaaaattaa taacactgat agtgctagtg
     1501 tagatcacta ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa agactgggcc
     1561 tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac tggctcacct
     1621 tcgggtgggc ctttctgcgt ttata
//