##gff-version 3
##sequence-region BBa_K077037 1 1645
BBa_K077037	.	promoter	1	97	.	+	0	ID=BBa_R0065;Name=BBa_R0065
BBa_K077037	.	promoter	47	52	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_K077037	.	non_covalent_binding_site	6	25	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_K077037	.	non_covalent_binding_site	57	73	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_K077037	.	engineered_region	1	97	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_K077037	.	promoter	94	97	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_K077037	.	non_covalent_binding_site	81	97	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_K077037	.	promoter	71	76	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_K077037	.	promoter	58	58	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_K077037	.	engineered_region	106	1645	.	+	0	ID=BBa_K077124;Name=BBa_K077124
BBa_K077037	.	engineered_region	648	1645	.	+	0	ID=BBa_S03119;Name=BBa_S03119;Parent=BBa_K077124
BBa_K077037	.	promoter	648	701	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_S03119
BBa_K077037	.	promoter	667	672	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K077037	.	non_covalent_binding_site	648	666	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K077037	.	engineered_region	648	701	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K077037	.	promoter	690	695	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K077037	.	non_covalent_binding_site	673	691	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K077037	.	terminator	1605	1645	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03119
BBa_K077037	.	stem_loop	1612	1631	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K077037	.	engineered_region	1605	1645	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K077037	.	stop_codon	1638	1638	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K077037	.	polyA_site	1632	1645	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K077037	.	terminator	1517	1596	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03119
BBa_K077037	.	engineered_region	1517	1596	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K077037	.	stem_loop	1528	1571	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K077037	.	ribosome_entry_site	710	721	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03119
BBa_K077037	.	conserved_region	714	717	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K077037	.	CDS	728	1483	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S03119
BBa_K077037	.	engineered_tag	1484	1508	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K077037	.	engineered_region	728	729	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K077037	.	CDS	728	1477	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K077037	.	point_mutation	901	901	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K077037	.	engineered_region	728	1483	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K077037	.	point_mutation	1219	1219	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K077037	.	engineered_region	106	639	.	+	0	ID=BBa_E0435;Name=BBa_E0435;Parent=BBa_K077124
BBa_K077037	.	engineered_region	124	502	.	+	0	ID=BBa_E0035;Name=BBa_E0035;Parent=BBa_E0435
BBa_K077037	.	sequence_alteration	234	234	.	+	0	ID=annotation1959043;Name=G;Parent=BBa_E0035
BBa_K077037	.	sequence_alteration	201	201	.	+	0	ID=annotation1959039;Name=C;Parent=BBa_E0035
BBa_K077037	.	promoter	149	158	.	+	0	ID=annotation1959037;Name=T3 promoter;Parent=BBa_E0035
BBa_K077037	.	sequence_alteration	471	471	.	+	0	ID=annotation1959045;Name=inserted T;Parent=BBa_E0035
BBa_K077037	.	sequence_alteration	231	231	.	+	0	ID=annotation1959042;Name=C;Parent=BBa_E0035
BBa_K077037	.	engineered_tag	478	502	.	+	0	ID=annotation1959046;Name=barcode;Parent=BBa_E0035
BBa_K077037	.	sequence_alteration	204	204	.	+	0	ID=annotation1959040;Name=T;Parent=BBa_E0035
BBa_K077037	.	sequence_alteration	210	210	.	+	0	ID=annotation1959041;Name=A;Parent=BBa_E0035
BBa_K077037	.	sequence_feature	322	471	.	+	0	ID=annotation1959044;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_K077037	.	promoter	296	314	.	+	0	ID=annotation1959038;Name=T7 promoter;Parent=BBa_E0035
BBa_K077037	.	sequence_feature	124	138	.	+	0	ID=annotation1959035;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_K077037	.	CDS	124	477	.	+	0	ID=annotation1959036;Name=lacZ alpha;Parent=BBa_E0035
BBa_K077037	.	ribosome_entry_site	106	117	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0435
BBa_K077037	.	conserved_region	110	113	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K077037	.	terminator	511	639	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_E0435
BBa_K077037	.	terminator	599	639	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K077037	.	stem_loop	606	625	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K077037	.	engineered_region	599	639	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K077037	.	stop_codon	632	632	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K077037	.	polyA_site	626	639	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K077037	.	terminator	511	590	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K077037	.	engineered_region	511	590	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K077037	.	stem_loop	522	565	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
>BBa_K077037
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactatt
ttacctctggcggtgatatactagagaaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccct
cactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaat
tcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacg
cgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcaca
tccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaattaa
taacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggc
ctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgc
gtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaa
agaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagc
aataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatc
ctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaa
tttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaat
gctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattc
atacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttaca
tgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatca
aacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaa
tattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgcca
aagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgt
agatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgttt
gtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata