LOCUS       BBa_K077042             1077 bp    DNA     linear       22-May-2024
DEFINITION  cII under control of plac promotor
ACCESSION   BBa_K077042
VERSION     BBa_K077042.1  
FEATURES             Location/Qualifiers
     CDS             1..88
                     /label=end of LacI coding region (inactive)
     misc_feature    1..200
                     /label=BBa_R0010
     misc_feature    89..126
                     /label=CAP binding site
     promoter        137..142
                     /label=-35
     promoter        161..166
                     /label=-10
     misc_feature    166..200
                     /label=LacI binding site
     misc_feature    173..173
                     /label=start
     promoter        1..200
                     /label=BBa_R0010
     misc_feature    215..218
                     /label=conserved
     RBS             209..222
                     /label=BBa_B0031
     CDS             229..876
                     /label=cII p22
     misc_feature    877..909
                     /label=LVA
     misc_feature    910..915
                     /label=stop
     misc_feature    229..915
                     /label=BBa_C0053
     misc_feature    916..940
                     /label=Help:Barcodes
     CDS             229..915
                     /label=BBa_C0053
     stem_loop       960..1003
                     /label=stem_loop
     misc_feature    949..1028
                     /label=BBa_B0010
     terminator      949..1028
                     /label=BBa_B0010
     stem_loop       1044..1063
                     /label=T7 TE
     misc_feature    1070..1070
                     /label=stop
     polyA_site      1064..1077
                     /label=polya
     misc_feature    1037..1077
                     /label=BBa_B0012
     terminator      1037..1077
                     /label=BBa_B0012
     misc_feature    209..1077
                     /label=BBa_P0153
ORIGIN
        1 caatacgcaa accgcctctc cccgcgcgtt ggccgattca ttaatgcagc tggcacgaca
       61 ggtttcccga ctggaaagcg ggcagtgagc gcaacgcaat taatgtgagt tagctcactc
      121 attaggcacc ccaggcttta cactttatgc ttccggctcg tatgttgtgt ggaattgtga
      181 gcggataaca atttcacaca tactagagtc acacaggaaa cctactagat gaatacacaa
      241 ttgatgggtg agcgtattcg cgctcgaaga aaaaaactca agattagaca agccgctctt
      301 ggtaagatgg tgggagtgtc taatgttgca atatcgcaat gggagcgctc ggagactgag
      361 ccaaatgggg agaacctgtt ggcactttcg aaggctcttc agtgctcccc tgactatttg
      421 ctgaaaggag atttaagcca gacaaacgtt gcctatcata gtaggcatga gccaagagga
      481 tcataccctc ttatcagttg ggtaagcgca gggcaatgga tggaagctgt agaaccttat
      541 cacaagcgcg cgatagagaa ctggcacgac accactgtag attgttcaga agattcattt
      601 tggcttgatg tccaaggtga ctctatgaca gcaccggcag ggttaagcat tccagaagga
      661 atgataattc tggttgatcc cgaagtcgaa ccaagaaacg gcaagctggt tgttgcaaaa
      721 ttagaaggtg aaaacgaggc cacattcaaa aaattagtta tggatgcagg ccgaaagttt
      781 ttaaaaccat taaacccaca atatccgatg atagaaatca acggaaactg caaaatcatt
      841 ggcgtagttg ttgacgcaaa actcgcaaat cttccagctg caaacgacga aaactacgct
      901 ttagtagctt aataacactg atagtgctag tgtagatcac tactagagcc aggcatcaaa
      961 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
     1021 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttata
//