LOCUS       BBa_K077044             2029 bp    DNA     linear       21-May-2024
DEFINITION  AiiA undercontrol of cI and cI under control of HSL induction
ACCESSION   BBa_K077044
VERSION     BBa_K077044.1  
FEATURES             Location/Qualifiers
     promoter        15..20
                     /label=-35
     promoter        25..41
                     /label=OR1
     promoter        1..17
                     /label=OR2
     promoter        38..43
                     /label=-10
     misc_feature    1..49
                     /label=BBa_R1051
     promoter        1..49
                     /label=BBa_R1051
     misc_feature    62..65
                     /label=conserved
     RBS             58..69
                     /label=BBa_B0034
     misc_feature    76..78
                     /label=start
     misc_feature    859..864
                     /label=2
     misc_feature    826..858
                     /label=LVA
     CDS             76..825
                     /label=aiiA
     misc_feature    76..864
                     /label=BBa_C0060
     misc_feature    865..889
                     /label=Help:Barcodes
     CDS             76..864
                     /label=BBa_C0060
     stem_loop       909..952
                     /label=stem_loop
     misc_feature    898..977
                     /label=BBa_B0010
     terminator      898..977
                     /label=BBa_B0010
     stem_loop       993..1012
                     /label=T7 TE
     misc_feature    1019..1019
                     /label=stop
     polyA_site      1013..1026
                     /label=polya
     misc_feature    986..1026
                     /label=BBa_B0012
     terminator      986..1026
                     /label=BBa_B0012
     misc_feature    1035..1054
                     /label=LuxR/HSL
     promoter        1054..1059
                     /label=-35
     promoter        1076..1081
                     /label=-10
     misc_feature    1087..1087
                     /label=start
     misc_feature    1035..1089
                     /label=BBa_R0062
     promoter        1035..1089
                     /label=BBa_R0062
     misc_feature    1104..1107
                     /label=conserved
     RBS             1098..1111
                     /label=BBa_B0031
     CDS             1121..1828
                     /label=cI lambda
     misc_feature    1829..1861
                     /label=LVA
     misc_feature    1868..1892
                     /label=Help:Barcodes
     CDS             1118..1867
                     /label=BBa_C0051
     stem_loop       1912..1955
                     /label=stem_loop
     misc_feature    1901..1980
                     /label=BBa_B0010
     terminator      1901..1980
                     /label=BBa_B0010
     stem_loop       1996..2015
                     /label=T7 TE
     misc_feature    2022..2022
                     /label=stop
     polyA_site      2016..2029
                     /label=polya
     misc_feature    1989..2029
                     /label=BBa_B0012
     terminator      1989..2029
                     /label=BBa_B0012
ORIGIN
        1 taacaccgtg cgtgttgaca attttacctc tggcggtgat actggttgct actagagaaa
       61 gaggagaaat actagatgac agtaaagaag ctttatttcg tcccagcagg tcgttgtatg
      121 ttggatcatt cgtctgttaa tagtacatta acaccaggag aattattaga cttaccggtt
      181 tggtgttatc ttttggagac tgaagaagga cctattttag tagatacagg tatgccagaa
      241 agtgcagtta ataatgaagg tctttttaac ggtacatttg tcgaagggca ggttttaccg
      301 aaaatgactg aagaagatag aatcgtgaat attttaaaac gggttggtta tgagccggaa
      361 gaccttcttt atattattag ttctcacttg cattttgatc atgcaggagg aaatggcgct
      421 tttataaata caccaatcat tgtacagcgt gctgaatatg aggcggcgca gcatagcgaa
      481 gaatatttga aagaatgtat attgccgaat ttaaactaca aaatcattga aggtgattat
      541 gaagtcgtac caggagttca attattgcat acaccaggcc atactccagg gcatcaatcg
      601 ctattaattg agacagaaaa atccggtcct gtattattaa cgattgatgc atcgtatacg
      661 aaagagaatt ttgaaaatga agtgccattt gcgggatttg attcagaatt agctttatct
      721 tcaattaaac gtttaaaaga agtggtgatg aaagagaagc cgattgtttt ctttggacat
      781 gatatagagc aggaaagggg atgtaaagtg ttccctgaat atatagctgc aaacgacgaa
      841 aactacgctt tagtagctta ataacgctga tagtgctagt gtagatcgct actagagcca
      901 ggcatcaaat aaaacgaaag gctcagtcga aagactgggc ctttcgtttt atctgttgtt
      961 tgtcggtgaa cgctctctac tagagtcaca ctggctcacc ttcgggtggg cctttctgcg
     1021 tttatatact agagacctgt aggatcgtac aggtttacgc aagaaaatgg tttgttatag
     1081 tcgaataaat actagagtca cacaggaaac ctactagatg agcacaaaaa agaaaccatt
     1141 aacacaagag cagcttgagg acgcacgtcg ccttaaagca atttatgaaa aaaagaaaaa
     1201 tgaacttggc ttatcccagg aatctgtcgc agacaagatg gggatggggc agtcaggcgt
     1261 tggtgcttta tttaatggca tcaatgcatt aaatgcttat aacgccgcat tgcttgcaaa
     1321 aattctcaaa gttagcgttg aagaatttag cccttcaatc gccagagaaa tctacgagat
     1381 gtatgaagcg gttagtatgc agccgtcact tagaagtgag tatgagtacc ctgttttttc
     1441 tcatgttcag gcagggatgt tctcacctga gcttagaacc tttaccaaag gtgatgcgga
     1501 gagatgggta agcacaacca aaaaagccag tgattctgca ttctggcttg aggttgaagg
     1561 taattccatg accgcaccaa caggctccaa gccaagcttt cctgacggaa tgttaattct
     1621 cgttgaccct gagcaggctg ttgagccagg tgatttctgc atagccagac ttgggggtga
     1681 tgagtttacc ttcaagaaac tgatcaggga tagcggtcag gtgtttttac aaccactaaa
     1741 cccacagtac ccaatgatcc catgcaatga gagttgttcc gttgtgggga aagttatcgc
     1801 tagtcagtgg cctgaagaga cgtttggcgc tgcaaacgac gaaaactacg ctttagtagc
     1861 ttaataacgc tgatagtgct agtgtagatc gctactagag ccaggcatca aataaaacga
     1921 aaggctcagt cgaaagactg ggcctttcgt tttatctgtt gtttgtcggt gaacgctctc
     1981 tactagagtc acactggctc accttcgggt gggcctttct gcgtttata
//