LOCUS       BBa_K077047             1031 bp    DNA     linear       25-Nov-2024
DEFINITION  AiiA constitutive produced
ACCESSION   BBa_K077047
VERSION     BBa_K077047.1  
FEATURES             Location/Qualifiers
     misc_feature    1..19
                     /label=TetR 1
     misc_feature    1..54
                     /label=BBa_R0040
     promoter        20..25
                     /label=-35
     misc_feature    26..44
                     /label=TetR 2
     promoter        43..48
                     /label=-10
     promoter        1..54
                     /label=BBa_R0040
     misc_feature    67..70
                     /label=conserved
     RBS             63..74
                     /label=BBa_B0034
     misc_feature    81..83
                     /label=start
     misc_feature    864..869
                     /label=2
     misc_feature    831..863
                     /label=LVA
     CDS             81..830
                     /label=aiiA
     misc_feature    81..869
                     /label=BBa_C0060
     misc_feature    870..894
                     /label=Help:Barcodes
     CDS             81..869
                     /label=BBa_C0060
     stem_loop       914..957
                     /label=stem_loop
     misc_feature    903..982
                     /label=BBa_B0010
     terminator      903..982
                     /label=BBa_B0010
     stem_loop       998..1017
                     /label=T7 TE
     misc_feature    1024..1024
                     /label=stop
     polyA_site      1018..1031
                     /label=polya
     misc_feature    991..1031
                     /label=BBa_B0012
     terminator      991..1031
                     /label=BBa_B0012
ORIGIN
        1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag
       61 agaaagagga gaaatactag atgacagtaa agaagcttta tttcgtccca gcaggtcgtt
      121 gtatgttgga tcattcgtct gttaatagta cattaacacc aggagaatta ttagacttac
      181 cggtttggtg ttatcttttg gagactgaag aaggacctat tttagtagat acaggtatgc
      241 cagaaagtgc agttaataat gaaggtcttt ttaacggtac atttgtcgaa gggcaggttt
      301 taccgaaaat gactgaagaa gatagaatcg tgaatatttt aaaacgggtt ggttatgagc
      361 cggaagacct tctttatatt attagttctc acttgcattt tgatcatgca ggaggaaatg
      421 gcgcttttat aaatacacca atcattgtac agcgtgctga atatgaggcg gcgcagcata
      481 gcgaagaata tttgaaagaa tgtatattgc cgaatttaaa ctacaaaatc attgaaggtg
      541 attatgaagt cgtaccagga gttcaattat tgcatacacc aggccatact ccagggcatc
      601 aatcgctatt aattgagaca gaaaaatccg gtcctgtatt attaacgatt gatgcatcgt
      661 atacgaaaga gaattttgaa aatgaagtgc catttgcggg atttgattca gaattagctt
      721 tatcttcaat taaacgttta aaagaagtgg tgatgaaaga gaagccgatt gttttctttg
      781 gacatgatat agagcaggaa aggggatgta aagtgttccc tgaatatata gctgcaaacg
      841 acgaaaacta cgctttagta gcttaataac gctgatagtg ctagtgtaga tcgctactag
      901 agccaggcat caaataaaac gaaaggctca gtcgaaagac tgggcctttc gttttatctg
      961 ttgtttgtcg gtgaacgctc tctactagag tcacactggc tcaccttcgg gtgggccttt
     1021 ctgcgtttat a
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