##gff-version 3
##sequence-region BBa_K077048 1 1864
BBa_K077048	.	terminator	811	858	.	+	0	ID=BBa_B0016;Name=BBa_B0016
BBa_K077048	.	promoter	867	920	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K077048	.	promoter	886	891	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K077048	.	non_covalent_binding_site	892	910	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K077048	.	engineered_region	867	920	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K077048	.	non_covalent_binding_site	867	885	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K077048	.	promoter	909	914	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K077048	.	ribosome_entry_site	929	940	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K077048	.	conserved_region	933	936	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K077048	.	CDS	947	1702	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K077048	.	CDS	947	1696	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K077048	.	engineered_region	947	1702	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K077048	.	engineered_tag	1703	1727	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K077048	.	point_mutation	1120	1120	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K077048	.	engineered_region	947	948	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K077048	.	point_mutation	1438	1438	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K077048	.	terminator	1824	1864	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K077048	.	engineered_region	1824	1864	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K077048	.	stop_codon	1857	1857	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K077048	.	stem_loop	1831	1850	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K077048	.	polyA_site	1851	1864	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K077048	.	CDS	83	802	.	+	0	ID=BBa_E0040;Name=BBa_E0040
BBa_K077048	.	CDS	83	802	.	+	0	ID=annotation1934520;Name=GFP protein;Parent=BBa_E0040
BBa_K077048	.	terminator	1736	1815	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K077048	.	stem_loop	1747	1790	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K077048	.	engineered_region	1736	1815	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K077048	.	ribosome_entry_site	64	76	.	+	0	ID=BBa_B0032;Name=BBa_B0032
BBa_K077048	.	ribosome_entry_site	70	73	.	+	0	ID=annotation1710;Name=RBS;Parent=BBa_B0032
BBa_K077048	.	engineered_region	64	76	.	+	0	ID=annotation7027;Name=BBa_B0032;Parent=BBa_B0032
BBa_K077048	.	ribosome_entry_site	64	76	.	+	0	ID=annotation1709;Name=RBS-3\Weak;Parent=BBa_B0032
BBa_K077048	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K077048	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
BBa_K077048	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K077048	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K077048	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K077048	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
>BBa_K077048
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagtcacacaggaaagtac
tagatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcaca
aattttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaa
actacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatg
aaacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacggga
actacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaa
agaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaa
aagaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaac
aaaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaaga
tcccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaacta
tacaaataataatactagagctagcataaccccttggggcctctaaacgggtcttgaggggttttttgtactagagtcc
ctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagat
gaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaa
tgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaat
ctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcc
tatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatct
ccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggct
tcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacatacc
attaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaa
agagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagc
gtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaat
tttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagcca
ggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctcta
ctagagtcacactggctcaccttcgggtgggcctttctgcgtttata