LOCUS       BBa_K077124             1540 bp    DNA     linear       23-May-2024
DEFINITION  Test construct for HSL/LuxR promotors, with LacZalpha output
ACCESSION   BBa_K077124
VERSION     BBa_K077124.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..33
                     /label=lacZ gene fragment
     CDS             19..372
                     /label=lacZ alpha
     promoter        44..53
                     /label=T3 promoter
     promoter        191..209
                     /label=T7 promoter
     misc_feature    96..96
                     /label=C
     misc_feature    99..99
                     /label=T
     misc_feature    105..105
                     /label=A
     misc_feature    126..126
                     /label=C
     misc_feature    129..129
                     /label=G
     misc_feature    217..366
                     /label=lacZ gene fragment
     misc_feature    366..366
                     /label=inserted T
     misc_feature    373..397
                     /label=barcode
     misc_feature    19..397
                     /label=BBa_E0035
     stem_loop       417..460
                     /label=stem_loop
     misc_feature    406..485
                     /label=BBa_B0010
     terminator      406..485
                     /label=BBa_B0010
     stem_loop       501..520
                     /label=T7 TE
     misc_feature    527..527
                     /label=stop
     polyA_site      521..534
                     /label=polya
     misc_feature    494..534
                     /label=BBa_B0012
     terminator      494..534
                     /label=BBa_B0012
     terminator      406..534
                     /label=BBa_B0015
     misc_feature    1..534
                     /label=BBa_E0435
     misc_feature    543..561
                     /label=TetR 1
     misc_feature    543..596
                     /label=BBa_R0040
     promoter        562..567
                     /label=-35
     misc_feature    568..586
                     /label=TetR 2
     promoter        585..590
                     /label=-10
     promoter        543..596
                     /label=BBa_R0040
     misc_feature    609..612
                     /label=conserved
     RBS             605..616
                     /label=BBa_B0034
     misc_feature    623..624
                     /label=prefix
     misc_feature    796..796
                     /label=T
     misc_feature    1114..1114
                     /label=A
     CDS             623..1372
                     /label=luxR
     misc_feature    623..1378
                     /label=BBa_C0062
     misc_feature    1379..1403
                     /label=Help:Barcodes
     CDS             623..1378
                     /label=BBa_C0062
     stem_loop       1423..1466
                     /label=stem_loop
     misc_feature    1412..1491
                     /label=BBa_B0010
     terminator      1412..1491
                     /label=BBa_B0010
     stem_loop       1507..1526
                     /label=T7 TE
     misc_feature    1533..1533
                     /label=stop
     polyA_site      1527..1540
                     /label=polya
     misc_feature    1500..1540
                     /label=BBa_B0012
     terminator      1500..1540
                     /label=BBa_B0012
     misc_feature    543..1540
                     /label=BBa_S03119
ORIGIN
        1 aaagaggaga aatactagat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
       61 gggaacaaaa gctggagctc caccgcggtg gcggcagcac tagagctagt ggatcccccg
      121 ggctgtagaa attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta
      181 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
      241 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      301 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      361 ctgaattaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa
      421 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      481 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
      541 agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact gagcactact
      601 agagaaagag gagaaatact agatgaaaaa cataaatgcc gacgacacat acagaataat
      661 taataaaatt aaagcttgta gaagcaataa tgatattaat caatgcttat ctgatatgac
      721 taaaatggta cattgtgaat attatttact cgcgatcatt tatcctcatt ctatggttaa
      781 atctgatatt tcaatcctag ataattaccc taaaaaatgg aggcaatatt atgatgacgc
      841 taatttaata aaatatgatc ctatagtaga ttattctaac tccaatcatt caccaattaa
      901 ttggaatata tttgaaaaca atgctgtaaa taaaaaatct ccaaatgtaa ttaaagaagc
      961 gaaaacatca ggtcttatca ctgggtttag tttccctatt catacggcta acaatggctt
     1021 cggaatgctt agttttgcac attcagaaaa agacaactat atagatagtt tatttttaca
     1081 tgcgtgtatg aacataccat taattgttcc ttctctagtt gataattatc gaaaaataaa
     1141 tatagcaaat aataaatcaa acaacgattt aaccaaaaga gaaaaagaat gtttagcgtg
     1201 ggcatgcgaa ggaaaaagct cttgggatat ttcaaaaata ttaggttgca gtgagcgtac
     1261 tgtcactttc catttaacca atgcgcaaat gaaactcaat acaacaaacc gctgccaaag
     1321 tatttctaaa gcaattttaa caggagcaat tgattgccca tactttaaaa attaataaca
     1381 ctgatagtgc tagtgtagat cactactaga gccaggcatc aaataaaacg aaaggctcag
     1441 tcgaaagact gggcctttcg ttttatctgt tgtttgtcgg tgaacgctct ctactagagt
     1501 cacactggct caccttcggg tgggcctttc tgcgtttata
//