LOCUS       BBa_K077125             2347 bp    DNA     linear       22-Dec-2024
DEFINITION  Test construct for HSL/LuxR or LasR promotors, with LacZalpha 
            output
ACCESSION   BBa_K077125
VERSION     BBa_K077125.1  
FEATURES             Location/Qualifiers
     misc_feature    5..8
                     /label=conserved
     RBS             1..12
                     /label=BBa_B0034
     misc_feature    19..33
                     /label=lacZ gene fragment
     CDS             19..372
                     /label=lacZ alpha
     promoter        44..53
                     /label=T3 promoter
     promoter        191..209
                     /label=T7 promoter
     misc_feature    96..96
                     /label=C
     misc_feature    99..99
                     /label=T
     misc_feature    105..105
                     /label=A
     misc_feature    126..126
                     /label=C
     misc_feature    129..129
                     /label=G
     misc_feature    217..366
                     /label=lacZ gene fragment
     misc_feature    366..366
                     /label=inserted T
     misc_feature    373..397
                     /label=barcode
     misc_feature    19..397
                     /label=BBa_E0035
     stem_loop       417..460
                     /label=stem_loop
     misc_feature    406..485
                     /label=BBa_B0010
     terminator      406..485
                     /label=BBa_B0010
     stem_loop       501..520
                     /label=T7 TE
     misc_feature    527..527
                     /label=stop
     polyA_site      521..534
                     /label=polya
     misc_feature    494..534
                     /label=BBa_B0012
     terminator      494..534
                     /label=BBa_B0012
     misc_feature    543..561
                     /label=TetR 1
     misc_feature    543..596
                     /label=BBa_R0040
     promoter        562..567
                     /label=-35
     misc_feature    568..586
                     /label=TetR 2
     promoter        585..590
                     /label=-10
     promoter        543..596
                     /label=BBa_R0040
     misc_feature    609..612
                     /label=conserved
     RBS             605..616
                     /label=BBa_B0034
     misc_feature    623..624
                     /label=prefix
     misc_feature    796..796
                     /label=T
     misc_feature    1114..1114
                     /label=A
     CDS             623..1372
                     /label=luxR
     misc_feature    623..1378
                     /label=BBa_C0062
     misc_feature    1379..1403
                     /label=Help:Barcodes
     CDS             623..1378
                     /label=BBa_C0062
     misc_feature    1416..1419
                     /label=conserved
     RBS             1412..1423
                     /label=BBa_B0034
     CDS             1430..2146
                     /label=lasR
     misc_feature    2147..2179
                     /label=LVA
     misc_feature    1909..1909
                     /label=G
     misc_feature    2186..2210
                     /label=Help:Barcodes
     CDS             1430..2185
                     /label=BBa_C0079
     stem_loop       2230..2273
                     /label=stem_loop
     misc_feature    2219..2298
                     /label=BBa_B0010
     terminator      2219..2298
                     /label=BBa_B0010
     stem_loop       2314..2333
                     /label=T7 TE
     misc_feature    2340..2340
                     /label=stop
     polyA_site      2334..2347
                     /label=polya
     misc_feature    2307..2347
                     /label=BBa_B0012
     terminator      2307..2347
                     /label=BBa_B0012
ORIGIN
        1 aaagaggaga aatactagat gaccatgatt acgccaagcg cgcaattaac cctcactaaa
       61 gggaacaaaa gctggagctc caccgcggtg gcggcagcac tagagctagt ggatcccccg
      121 ggctgtagaa attcgatatc aagcttatcg ataccgtcga cctcgagggg gggcccggta
      181 cccaattcgc cctatagtga gtcgtattac gcgcgctcac tggccgtcgt tttacaacgt
      241 cgtgactggg aaaaccctgg cgttacccaa cttaatcgcc ttgcagcaca tccccctttc
      301 gccagctggc gtaatagcga agaggcccgc accgatcgcc cttcccaaca gttgcgcagc
      361 ctgaattaat aacgctgata gtgctagtgt agatcgctac tagagccagg catcaaataa
      421 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg
      481 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tatatactag
      541 agtccctatc agtgatagag attgacatcc ctatcagtga tagagatact gagcactact
      601 agagaaagag gagaaatact agatgaaaaa cataaatgcc gacgacacat acagaataat
      661 taataaaatt aaagcttgta gaagcaataa tgatattaat caatgcttat ctgatatgac
      721 taaaatggta cattgtgaat attatttact cgcgatcatt tatcctcatt ctatggttaa
      781 atctgatatt tcaatcctag ataattaccc taaaaaatgg aggcaatatt atgatgacgc
      841 taatttaata aaatatgatc ctatagtaga ttattctaac tccaatcatt caccaattaa
      901 ttggaatata tttgaaaaca atgctgtaaa taaaaaatct ccaaatgtaa ttaaagaagc
      961 gaaaacatca ggtcttatca ctgggtttag tttccctatt catacggcta acaatggctt
     1021 cggaatgctt agttttgcac attcagaaaa agacaactat atagatagtt tatttttaca
     1081 tgcgtgtatg aacataccat taattgttcc ttctctagtt gataattatc gaaaaataaa
     1141 tatagcaaat aataaatcaa acaacgattt aaccaaaaga gaaaaagaat gtttagcgtg
     1201 ggcatgcgaa ggaaaaagct cttgggatat ttcaaaaata ttaggttgca gtgagcgtac
     1261 tgtcactttc catttaacca atgcgcaaat gaaactcaat acaacaaacc gctgccaaag
     1321 tatttctaaa gcaattttaa caggagcaat tgattgccca tactttaaaa attaataaca
     1381 ctgatagtgc tagtgtagat cactactaga gaaagaggag aaatactaga tggccttggt
     1441 tgacggtttt cttgagctgg aacgctcaag tggaaaattg gagtggagcg ccatcctcca
     1501 gaagatggcg agcgaccttg gattctcgaa gatcctgttc ggcctgttgc ctaaggacag
     1561 ccaggactac gagaacgcct tcatcgtcgg caactacccg gccgcctggc gcgagcatta
     1621 cgaccgggct ggctacgcgc gggtcgaccc gacggtcagt cactgtaccc agagcgtact
     1681 gccgattttc tgggaaccgt ccatctacca gacgcgaaag cagcacgagt tcttcgagga
     1741 agcctcggcc gccggcctgg tgtatgggct gaccatgccg ctgcatggtg ctcgcggcga
     1801 actcggcgcg ctgagcctca gcgtggaagc ggaaaaccgg gccgaggcca accgtttcat
     1861 agagtcggtc ctgccgaccc tgtggatgct caaggactac gcactgcaaa gcggtgccgg
     1921 actggccttc gaacatccgg tcagcaaacc ggtggttctg accagccggg agaaggaagt
     1981 gttgcagtgg tgcgccatcg gcaagaccag ttgggagata tcggttatct gcaactgctc
     2041 ggaagccaat gtgaacttcc atatgggaaa tattcggcgg aagttcggtg tgacctcccg
     2101 ccgcgtagcg gccattatgg ccgttaattt gggtcttatt actctcgctg caaacgacga
     2161 aaactacgct ttagtagctt aataacactg atagtgctag tgtagatcac tactagagcc
     2221 aggcatcaaa taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt
     2281 ttgtcggtga acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc
     2341 gtttata
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