##gff-version 3
##sequence-region BBa_K077125 1 2347
BBa_K077125	.	ribosome_entry_site	1412	1423	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K077125	.	conserved_region	1416	1419	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K077125	.	CDS	623	1378	.	+	0	ID=BBa_C0062;Name=BBa_C0062
BBa_K077125	.	engineered_tag	1379	1403	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K077125	.	engineered_region	623	624	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K077125	.	CDS	623	1372	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K077125	.	point_mutation	796	796	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K077125	.	engineered_region	623	1378	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K077125	.	point_mutation	1114	1114	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K077125	.	promoter	543	596	.	+	0	ID=BBa_R0040;Name=BBa_R0040
BBa_K077125	.	promoter	562	567	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K077125	.	non_covalent_binding_site	543	561	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K077125	.	engineered_region	543	596	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K077125	.	promoter	585	590	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K077125	.	non_covalent_binding_site	568	586	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K077125	.	ribosome_entry_site	605	616	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K077125	.	conserved_region	609	612	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K077125	.	terminator	2219	2298	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K077125	.	engineered_region	2219	2298	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K077125	.	stem_loop	2230	2273	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K077125	.	engineered_region	19	397	.	+	0	ID=BBa_E0035;Name=BBa_E0035
BBa_K077125	.	sequence_alteration	129	129	.	+	0	ID=annotation1959043;Name=G;Parent=BBa_E0035
BBa_K077125	.	sequence_alteration	96	96	.	+	0	ID=annotation1959039;Name=C;Parent=BBa_E0035
BBa_K077125	.	promoter	44	53	.	+	0	ID=annotation1959037;Name=T3 promoter;Parent=BBa_E0035
BBa_K077125	.	sequence_alteration	366	366	.	+	0	ID=annotation1959045;Name=inserted T;Parent=BBa_E0035
BBa_K077125	.	sequence_alteration	126	126	.	+	0	ID=annotation1959042;Name=C;Parent=BBa_E0035
BBa_K077125	.	engineered_tag	373	397	.	+	0	ID=annotation1959046;Name=barcode;Parent=BBa_E0035
BBa_K077125	.	sequence_alteration	99	99	.	+	0	ID=annotation1959040;Name=T;Parent=BBa_E0035
BBa_K077125	.	sequence_alteration	105	105	.	+	0	ID=annotation1959041;Name=A;Parent=BBa_E0035
BBa_K077125	.	sequence_feature	217	366	.	+	0	ID=annotation1959044;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_K077125	.	promoter	191	209	.	+	0	ID=annotation1959038;Name=T7 promoter;Parent=BBa_E0035
BBa_K077125	.	sequence_feature	19	33	.	+	0	ID=annotation1959035;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_K077125	.	CDS	19	372	.	+	0	ID=annotation1959036;Name=lacZ alpha;Parent=BBa_E0035
BBa_K077125	.	ribosome_entry_site	1	12	.	+	0	ID=BBa_B0034;Name=BBa_B0034
BBa_K077125	.	conserved_region	5	8	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K077125	.	terminator	406	485	.	+	0	ID=BBa_B0010;Name=BBa_B0010
BBa_K077125	.	engineered_region	406	485	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K077125	.	stem_loop	417	460	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K077125	.	CDS	1430	2185	.	+	0	ID=BBa_C0079;Name=BBa_C0079
BBa_K077125	.	engineered_tag	2186	2210	.	+	0	ID=annotation2213999;Name=Help:Barcodes;Parent=BBa_C0079
BBa_K077125	.	CDS	1430	2146	.	+	0	ID=annotation307935;Name=lasR;Parent=BBa_C0079
BBa_K077125	.	sequence_alteration	1909	1909	.	+	0	ID=annotation308006;Name=G;Parent=BBa_C0079
BBa_K077125	.	tag	2147	2179	.	+	0	ID=annotation307948;Name=LVA;Parent=BBa_C0079
BBa_K077125	.	terminator	494	534	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K077125	.	stem_loop	501	520	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K077125	.	engineered_region	494	534	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K077125	.	stop_codon	527	527	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K077125	.	polyA_site	521	534	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K077125	.	terminator	2307	2347	.	+	0	ID=BBa_B0012;Name=BBa_B0012
BBa_K077125	.	stem_loop	2314	2333	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K077125	.	engineered_region	2307	2347	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K077125	.	stop_codon	2340	2340	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K077125	.	polyA_site	2334	2347	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
>BBa_K077125
aaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagct
ccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtc
gacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaa
cgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaata
gcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatc
gctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcgg
tgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagt
gatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaaca
taaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatc
tgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatt
tcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtag
attattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgt
aattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatg
cttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattg
ttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaa
agaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtc
actttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacag
gagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagaaagaggagaa
atactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccag
aagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgcct
tcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcag
tcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgag
gaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcc
tcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaagga
ctacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaag
gaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtga
acttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttggg
tcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcacta
ctagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaa
cgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttata