BBa_K077125
type: ComponentDefinition
persistent identity: BBa K077125
display id: BBa_K077125
version: 1
was generated by: iGEM to SBOL conversion
description: Test construct for HSL/LuxR or LasR promotors, with LacZalpha output
created: 2008-07-27T11:00:00Z
modified: 2015-05-08T01:08:33Z
mutable provenance: BBa_E0435, BBa_K077015
owned by: james
owned by: myers
mutable description: Test construct for HSL/LuxR or LasR promotors, with LacZalpha output.
discontinued: true
dominant: false
group u list: _195_
m user id: 0
owner id: 2638
owning group id: 9
sample status: Discontinued
status: status/Deleted
bookmark: false
mutable notes: BBa_E0435, BBa_K077015
star: false
creator: Floor Hugenholtz
type: DnaRegion
role: partType/Composite
role: SO:0000804
component: BBa_B0012
component: BBa_B0010
component: BBa_C0079
component: BBa_B0034
component: BBa_E0035
component: BBa_C0062
component: BBa_R0040
sequence annotation: BBa_B0034
sequence annotation: BBa_E0035
sequence annotation: BBa_B0010
sequence annotation: BBa_B0012
sequence annotation: BBa_R0040
sequence annotation: BBa_C0062
sequence annotation: BBa_C0079
sequence: https://synbiohub.org/public/igem/BBa_K077125_sequence/1

BBa_B0012
type: Component
persistent identity: component1968729
display id: component1968729
version: 1
top level: BBa_K077125
definition: BBa_B0012
access: public

BBa_B0010
type: Component
persistent identity: component1968727
display id: component1968727
version: 1
top level: BBa_K077125
definition: BBa_B0010
access: public

BBa_C0079
type: Component
persistent identity: component1968725
display id: component1968725
version: 1
top level: BBa_K077125
definition: lasr
access: public

BBa_B0034
type: Component
persistent identity: component1968721
display id: component1968721
version: 1
top level: BBa_K077125
definition: BBa_B0034
access: public

BBa_B0010
type: Component
persistent identity: component1968701
display id: component1968701
version: 1
top level: BBa_K077125
definition: BBa_B0010
access: public

BBa_E0035
type: Component
persistent identity: component1968700
display id: component1968700
version: 1
top level: BBa_K077125
definition: BBa_E0035
access: public

BBa_B0034
type: Component
persistent identity: component1968687
display id: component1968687
version: 1
top level: BBa_K077125
definition: BBa_B0034
access: public

BBa_C0062
type: Component
persistent identity: component1968716
display id: component1968716
version: 1
top level: BBa_K077125
definition: luxr
access: public

BBa_B0034
type: Component
persistent identity: component1968713
display id: component1968713
version: 1
top level: BBa_K077125
definition: BBa_B0034
access: public

BBa_R0040
type: Component
persistent identity: component1968708
display id: component1968708
version: 1
top level: BBa_K077125
definition: p(tetR)
access: public

BBa_B0012
type: Component
persistent identity: component1968703
display id: component1968703
version: 1
top level: BBa_K077125
definition: BBa_B0012
access: public

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1968687
display id: annotation1968687
version: 1
top level: BBa_K077125
location: https://synbiohub.org/public/igem/BBa_K077125/annotation1968687/range1968687/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K077125/annotation1968687/range1968687/1
type: Range
persistent identity: range1968687
display id: range1968687
version: 1
top level: BBa_K077125
direction: direction/reverse
start: 1
end: 12
orientation: inline

BBa_E0035
type: SequenceAnnotation
persistent identity: annotation1968700
display id: annotation1968700
version: 1
top level: BBa_K077125
location: https://synbiohub.org/public/igem/BBa_K077125/annotation1968700/range1968700/1
component: BBa_E0035

https://synbiohub.org/public/igem/BBa_K077125/annotation1968700/range1968700/1
type: Range
persistent identity: range1968700
display id: range1968700
version: 1
top level: BBa_K077125
direction: direction/reverse
start: 19
end: 397
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1968701
display id: annotation1968701
version: 1
top level: BBa_K077125
location: https://synbiohub.org/public/igem/BBa_K077125/annotation1968701/range1968701/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K077125/annotation1968701/range1968701/1
type: Range
persistent identity: range1968701
display id: range1968701
version: 1
top level: BBa_K077125
direction: direction/forward
start: 406
end: 485
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1968703
display id: annotation1968703
version: 1
top level: BBa_K077125
location: https://synbiohub.org/public/igem/BBa_K077125/annotation1968703/range1968703/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K077125/annotation1968703/range1968703/1
type: Range
persistent identity: range1968703
display id: range1968703
version: 1
top level: BBa_K077125
direction: direction/forward
start: 494
end: 534
orientation: inline

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1968708
display id: annotation1968708
version: 1
top level: BBa_K077125
location: https://synbiohub.org/public/igem/BBa_K077125/annotation1968708/range1968708/1
component: BBa_R0040

https://synbiohub.org/public/igem/BBa_K077125/annotation1968708/range1968708/1
type: Range
persistent identity: range1968708
display id: range1968708
version: 1
top level: BBa_K077125
direction: direction/forward
start: 543
end: 596
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1968713
display id: annotation1968713
version: 1
top level: BBa_K077125
location: https://synbiohub.org/public/igem/BBa_K077125/annotation1968713/range1968713/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K077125/annotation1968713/range1968713/1
type: Range
persistent identity: range1968713
display id: range1968713
version: 1
top level: BBa_K077125
direction: direction/reverse
start: 605
end: 616
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation1968716
display id: annotation1968716
version: 1
top level: BBa_K077125
location: https://synbiohub.org/public/igem/BBa_K077125/annotation1968716/range1968716/1
component: BBa_C0062

https://synbiohub.org/public/igem/BBa_K077125/annotation1968716/range1968716/1
type: Range
persistent identity: range1968716
display id: range1968716
version: 1
top level: BBa_K077125
direction: direction/forward
start: 623
end: 1378
orientation: inline

BBa_B0034
type: SequenceAnnotation
persistent identity: annotation1968721
display id: annotation1968721
version: 1
top level: BBa_K077125
location: https://synbiohub.org/public/igem/BBa_K077125/annotation1968721/range1968721/1
component: BBa_B0034

https://synbiohub.org/public/igem/BBa_K077125/annotation1968721/range1968721/1
type: Range
persistent identity: range1968721
display id: range1968721
version: 1
top level: BBa_K077125
direction: direction/reverse
start: 1412
end: 1423
orientation: inline

BBa_C0079
type: SequenceAnnotation
persistent identity: annotation1968725
display id: annotation1968725
version: 1
top level: BBa_K077125
location: https://synbiohub.org/public/igem/BBa_K077125/annotation1968725/range1968725/1
component: BBa_C0079

https://synbiohub.org/public/igem/BBa_K077125/annotation1968725/range1968725/1
type: Range
persistent identity: range1968725
display id: range1968725
version: 1
top level: BBa_K077125
direction: direction/reverse
start: 1430
end: 2185
orientation: inline

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation1968727
display id: annotation1968727
version: 1
top level: BBa_K077125
location: https://synbiohub.org/public/igem/BBa_K077125/annotation1968727/range1968727/1
component: BBa_B0010

https://synbiohub.org/public/igem/BBa_K077125/annotation1968727/range1968727/1
type: Range
persistent identity: range1968727
display id: range1968727
version: 1
top level: BBa_K077125
direction: direction/forward
start: 2219
end: 2298
orientation: inline

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation1968729
display id: annotation1968729
version: 1
top level: BBa_K077125
location: https://synbiohub.org/public/igem/BBa_K077125/annotation1968729/range1968729/1
component: BBa_B0012

https://synbiohub.org/public/igem/BBa_K077125/annotation1968729/range1968729/1
type: Range
persistent identity: range1968729
display id: range1968729
version: 1
top level: BBa_K077125
direction: direction/forward
start: 2307
end: 2347
orientation: inline

BBa_B0034
type: ComponentDefinition
persistent identity: BBa B0034
display id: BBa_B0034
version: 1
was generated by: iGEM to SBOL conversion
description: RBS (Elowitz 1999) -- defines RBS efficiency
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: RBS based on Elowitz repressilator.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Varies from -6 to +1 region from original sequence to accomodate BioBricks suffix. No secondary structures are formed in the given RBS region. Users should check for secondary structures induced in the RBS by upstream and downstream elements in the +50 to -50 region, as such structures will greatly affect the strength of the RBS. Contact info for this part: Brian Chow
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr IAP, 2003.
type: DnaRegion
role: partType/RBS
role: SO:0000139
sequence annotation: conserved
sequence: https://synbiohub.org/public/igem/BBa_B0034_sequence/1

conserved
type: SequenceAnnotation
persistent identity: annotation23325
display id: annotation23325
version: 1
top level: BBa_B0034
location: https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
role: SO:0000330
role: feature/conserved

https://synbiohub.org/public/igem/BBa_B0034/annotation23325/range23325/1
type: Range
persistent identity: range23325
display id: range23325
version: 1
top level: BBa_B0034
direction: direction/forward
start: 5
end: 8
orientation: inline

BBa_B0012
type: ComponentDefinition
persistent identity: BBa B0012
display id: BBa_B0012
version: 1
was generated by: iGEM to SBOL conversion
description: TE from coliphageT7
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:20Z
mutable provenance: Derived from the TE terminator of T7 bacteriophage between Genes 1.3 and 1.4 <genbank>V01146</genbank>.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Transcription terminator for the E.coli RNA polymerase.
discontinued: false
dominant: false
experience: experience/Fails
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Suggested by Sri Kosuri and Drew Endy as a high efficiency terminator. The 5' end cutoff was placed immediately after the TAA stop codon and the 3' end cutoff was placed just prior to the RBS of Gene 1.4 (before AAGGAG). Use anywhere transcription should be stopped when the gene of interest is upstream of this terminator.
star: false
creator: Reshma Shetty
type: DnaRegion
role: SO:0000141
role: partType/Terminator
sequence annotation: BBa_B0012
sequence annotation: T7 TE
sequence annotation: polya
sequence annotation: stop
sequence: https://synbiohub.org/public/igem/BBa_B0012_sequence/1

BBa_B0012
type: SequenceAnnotation
persistent identity: annotation7020
display id: annotation7020
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_B0012/annotation7020/range7020/1
type: Range
persistent identity: range7020
display id: range7020
version: 1
top level: BBa_B0012
direction: direction/forward
start: 1
end: 41
orientation: inline

T7 TE
type: SequenceAnnotation
persistent identity: annotation1686
display id: annotation1686
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
role: SO:0000313
role: feature/stem loop

https://synbiohub.org/public/igem/BBa_B0012/annotation1686/range1686/1
type: Range
persistent identity: range1686
display id: range1686
version: 1
top level: BBa_B0012
direction: direction/forward
start: 8
end: 27
orientation: inline

polya
type: SequenceAnnotation
persistent identity: annotation1690
display id: annotation1690
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
role: feature/polya
role: SO:0000553

https://synbiohub.org/public/igem/BBa_B0012/annotation1690/range1690/1
type: Range
persistent identity: range1690
display id: range1690
version: 1
top level: BBa_B0012
direction: direction/forward
start: 28
end: 41
orientation: inline

stop
type: SequenceAnnotation
persistent identity: annotation1687
display id: annotation1687
version: 1
top level: BBa_B0012
location: https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
role: feature/stop
role: SO:0000319

https://synbiohub.org/public/igem/BBa_B0012/annotation1687/range1687/1
type: Range
persistent identity: range1687
display id: range1687
version: 1
top level: BBa_B0012
direction: direction/forward
start: 34
end: 34
orientation: inline

BBa_B0010
type: ComponentDefinition
persistent identity: BBa B0010
display id: BBa_B0010
version: 1
was generated by: iGEM to SBOL conversion
description: T1 from E. coli rrnB
created: 2003-11-19T12:00:00Z
modified: 2015-08-31T04:07:20Z
owned by: james
owned by: myers
mutable description: Transcriptional terminator consisting of a 64 bp stem-loop.
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
star: true
creator: Randy Rettberg
type: DnaRegion
role: partType/Terminator
role: SO:0000141
sequence annotation: BBa_B0010
sequence annotation: stem_loop
sequence: https://synbiohub.org/public/igem/BBa_B0010_sequence/1

BBa_B0010
type: SequenceAnnotation
persistent identity: annotation7018
display id: annotation7018
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_B0010/annotation7018/range7018/1
type: Range
persistent identity: range7018
display id: range7018
version: 1
top level: BBa_B0010
direction: direction/forward
start: 1
end: 80
orientation: inline

stem_loop
type: SequenceAnnotation
persistent identity: annotation4184
display id: annotation4184
version: 1
top level: BBa_B0010
location: https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
role: feature/stem loop
role: SO:0000313

https://synbiohub.org/public/igem/BBa_B0010/annotation4184/range4184/1
type: Range
persistent identity: range4184
display id: range4184
version: 1
top level: BBa_B0010
direction: direction/forward
start: 12
end: 55
orientation: inline

p(tetR)
type: ComponentDefinition
persistent identity: BBa R0040
display id: BBa_R0040
version: 1
was generated by: iGEM to SBOL conversion
description: TetR repressible promoter
created: 2003-01-31T12:00:00Z
modified: 2015-05-08T01:14:14Z
mutable provenance: Lutz, R., Bujard, H., Nucleic Acids Research (1997) 25, 1203-1210.
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: Sequence for pTet inverting regulator driven by the TetR protein.
discontinued: false
dominant: true
experience: experience/Works
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: BBa_R0040 TetR-Regulated Promoter is based on a cI promoter. It has been modified to include two TetR binding sites and the BioBrick standard assembly head and tail restriction sites.
star: true
creator: June Rhee, Connie Tao, Ty Thomson, Louis Waldman
type: DnaRegion
role: SO:0000167
role: partType/Regulatory
sequence annotation: BBa_R0040
sequence annotation: TetR 1
sequence annotation: -35
sequence annotation: TetR 2
sequence annotation: -10
sequence: https://synbiohub.org/public/igem/BBa_R0040_sequence/1

BBa_R0040
type: SequenceAnnotation
persistent identity: annotation1986784
display id: annotation1986784
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
role: feature/BioBrick
role: SO:0000804

https://synbiohub.org/public/igem/BBa_R0040/annotation1986784/range1986784/1
type: Range
persistent identity: range1986784
display id: range1986784
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 54
orientation: inline

TetR 1
type: SequenceAnnotation
persistent identity: annotation1986783
display id: annotation1986783
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986783/range1986783/1
type: Range
persistent identity: range1986783
display id: range1986783
version: 1
top level: p(tetR)
direction: direction/forward
start: 1
end: 19
orientation: inline

-35
type: SequenceAnnotation
persistent identity: annotation1986785
display id: annotation1986785
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
role: SO:0000167
role: feature/promoter

https://synbiohub.org/public/igem/BBa_R0040/annotation1986785/range1986785/1
type: Range
persistent identity: range1986785
display id: range1986785
version: 1
top level: p(tetR)
direction: direction/forward
start: 20
end: 25
orientation: inline

TetR 2
type: SequenceAnnotation
persistent identity: annotation1986786
display id: annotation1986786
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
role: SO:0001091
role: feature/binding

https://synbiohub.org/public/igem/BBa_R0040/annotation1986786/range1986786/1
type: Range
persistent identity: range1986786
display id: range1986786
version: 1
top level: p(tetR)
direction: direction/forward
start: 26
end: 44
orientation: inline

-10
type: SequenceAnnotation
persistent identity: annotation1986787
display id: annotation1986787
version: 1
top level: p(tetR)
location: https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_R0040/annotation1986787/range1986787/1
type: Range
persistent identity: range1986787
display id: range1986787
version: 1
top level: p(tetR)
direction: direction/forward
start: 43
end: 48
orientation: inline

luxr
type: ComponentDefinition
persistent identity: BBa C0062
display id: BBa_C0062
version: 1
was generated by: iGEM to SBOL conversion
description: luxR repressor/activator, (no LVA?)
created: 2003-01-31T12:00:00Z
modified: 2015-08-31T04:07:23Z
mutable provenance: V. fischeri <genbank>AF170104</genbank>
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: In complex with HSL, LuxR binds to the Lux promoter, activating transcription from Pr <bb_part>BBa_R0062</bb_part>, and repressing transcription from Pl <bb_part>BBa_R0063</bb_part>. The lux cassette of V. fischeri contains a left and a right promoter. The right promoter gives weak constitutive expression of downstream genes.This expression is up-regulated by the action of the Lux activator, LuxR complexed to HSL. Two molecules of LuxR protein form a complex with two molecules the signalling compound homoserine lactone (HSL). This complex binds to a palindromic site on the promoter, increasing the rate of transcription.
discontinued: false
dominant: true
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: 2 silent point mutants were introduced in the coding sequence to remove internal XbaI and PstI sites. Mutation sites were chosen to replace codons commonly used in E. coli with codons used at a similar frequency.
star: true
creator: Vinay S Mahajan, Voichita D. Marinescu, Brian Chow, Alexander D Wissner-Gross and Peter Carr
type: DnaRegion
role: partType/Coding
role: SO:0000316
sequence annotation: luxR
sequence annotation: prefix
sequence annotation: BBa_C0062
sequence annotation: T
sequence annotation: A
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0062_sequence/1

luxR
type: SequenceAnnotation
persistent identity: annotation1766
display id: annotation1766
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0062/annotation1766/range1766/1
type: Range
persistent identity: range1766
display id: range1766
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 750
orientation: inline

prefix
type: SequenceAnnotation
persistent identity: annotation1762
display id: annotation1762
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation1762/range1762/1
type: Range
persistent identity: range1762
display id: range1762
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 2
orientation: inline

BBa_C0062
type: SequenceAnnotation
persistent identity: annotation7039
display id: annotation7039
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
role: SO:0000804
role: feature/BioBrick

https://synbiohub.org/public/igem/BBa_C0062/annotation7039/range7039/1
type: Range
persistent identity: range7039
display id: range7039
version: 1
top level: luxr
direction: direction/forward
start: 1
end: 756
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1764
display id: annotation1764
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1764/range1764/1
type: Range
persistent identity: range1764
display id: range1764
version: 1
top level: luxr
direction: direction/forward
start: 174
end: 174
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1765
display id: annotation1765
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
role: feature/s mutation
role: SO:1000008

https://synbiohub.org/public/igem/BBa_C0062/annotation1765/range1765/1
type: Range
persistent identity: range1765
display id: range1765
version: 1
top level: luxr
direction: direction/forward
start: 492
end: 492
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213986
display id: annotation2213986
version: 1
top level: luxr
location: https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0062/annotation2213986/range2213986/1
type: Range
persistent identity: range2213986
display id: range2213986
version: 1
top level: luxr
direction: direction/forward
start: 757
end: 781
orientation: inline

lasr
type: ComponentDefinition
persistent identity: BBa C0079
display id: BBa_C0079
version: 1
was generated by: iGEM to SBOL conversion
description: lasR activator from P. aeruginosa PAO1(+LVA)
created: 2004-01-27T12:00:00Z
modified: 2015-08-31T04:07:24Z
mutable provenance: Genbank: NC_002516 (www.ncbi.nlm.nih.gov)
owned by: james
owned by: myers
part status: Released HQ 2013
mutable description: coding region for lasR protein, which accepts chemical signal AI-1 (made by lasI protein)
discontinued: false
dominant: false
experience: experience/None
group u list: _1_
m user id: 0
owner id: 24
owning group id: 7
sample status: In stock
status: status/Available
bookmark: false
mutable notes: Mutated 480 from g to a to remove PstI site
star: true
creator: Alvin Carter Powers (Fighting Darwins)
type: DnaRegion
role: SO:0000316
role: partType/Coding
sequence annotation: lasR
sequence annotation: G
sequence annotation: LVA
sequence annotation: Help:Barcodes
sequence: https://synbiohub.org/public/igem/BBa_C0079_sequence/1

lasR
type: SequenceAnnotation
persistent identity: annotation307935
display id: annotation307935
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_C0079/annotation307935/range307935/1
type: Range
persistent identity: range307935
display id: range307935
version: 1
top level: lasr
direction: direction/forward
start: 1
end: 717
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation308006
display id: annotation308006
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_C0079/annotation308006/range308006/1
type: Range
persistent identity: range308006
display id: range308006
version: 1
top level: lasr
direction: direction/forward
start: 480
end: 480
orientation: inline

LVA
type: SequenceAnnotation
persistent identity: annotation307948
display id: annotation307948
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1
role: SO:0000324
role: feature/tag

https://synbiohub.org/public/igem/BBa_C0079/annotation307948/range307948/1
type: Range
persistent identity: range307948
display id: range307948
version: 1
top level: lasr
direction: direction/forward
start: 718
end: 750
orientation: inline

Help:Barcodes
type: SequenceAnnotation
persistent identity: annotation2213999
display id: annotation2213999
version: 1
top level: lasr
location: https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_C0079/annotation2213999/range2213999/1
type: Range
persistent identity: range2213999
display id: range2213999
version: 1
top level: lasr
direction: direction/forward
start: 757
end: 781
orientation: inline

BBa_E0035
type: ComponentDefinition
persistent identity: BBa E0035
display id: BBa_E0035
version: 1
was generated by: iGEM to SBOL conversion
description: LacZ alpha fragment; complements matching N-terminal deletion mutant (lacZ-omega)
created: 2007-12-21T12:00:00Z
modified: 2015-08-31T04:07:25Z
mutable provenance: This part is derived from BBa_E0033.
owned by: james
owned by: myers
mutable description: This part encodes the alpha fragment of the lacZ gene. To greatly reduce the number of bases, this is only a portion of the LacZ gene. It can be used as a detection system with N-terminal deletion mutants of lacZ. Combination of lacZ-alpha with such a deletion mutant protein restores enzyme activity that can be assayed colorimetrically or more sensitively with chemiluminescent substrates.
discontinued: false
dominant: false
group u list: _41_
m user id: 0
owner id: 126
owning group id: 162
sample status: Not in stock
status: status/Unavailable
bookmark: false
mutable notes: It has the additional T needed to ensure that the double stop codon is in frame.
star: false
creator: Reshma Shetty
type: DnaRegion
role: partType/Reporter
role: SO:0000804
sequence annotation: lacZ gene fragment
sequence annotation: lacZ alpha
sequence annotation: T3 promoter
sequence annotation: C
sequence annotation: T
sequence annotation: A
sequence annotation: G
sequence annotation: T7 promoter
sequence annotation: inserted T
sequence annotation: barcode
sequence: https://synbiohub.org/public/igem/BBa_E0035_sequence/1

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1959035
display id: annotation1959035
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959035/range1959035/1
role: feature/misc
role: SO:0000110

https://synbiohub.org/public/igem/BBa_E0035/annotation1959035/range1959035/1
type: Range
persistent identity: range1959035
display id: range1959035
version: 1
top level: BBa_E0035
direction: direction/forward
start: 1
end: 15
orientation: inline

lacZ alpha
type: SequenceAnnotation
persistent identity: annotation1959036
display id: annotation1959036
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959036/range1959036/1
role: feature/cds
role: SO:0000316

https://synbiohub.org/public/igem/BBa_E0035/annotation1959036/range1959036/1
type: Range
persistent identity: range1959036
display id: range1959036
version: 1
top level: BBa_E0035
direction: direction/forward
start: 1
end: 354
orientation: inline

T3 promoter
type: SequenceAnnotation
persistent identity: annotation1959037
display id: annotation1959037
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959037/range1959037/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_E0035/annotation1959037/range1959037/1
type: Range
persistent identity: range1959037
display id: range1959037
version: 1
top level: BBa_E0035
direction: direction/forward
start: 26
end: 35
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1959039
display id: annotation1959039
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959039/range1959039/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0035/annotation1959039/range1959039/1
type: Range
persistent identity: range1959039
display id: range1959039
version: 1
top level: BBa_E0035
direction: direction/forward
start: 78
end: 78
orientation: inline

T
type: SequenceAnnotation
persistent identity: annotation1959040
display id: annotation1959040
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959040/range1959040/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0035/annotation1959040/range1959040/1
type: Range
persistent identity: range1959040
display id: range1959040
version: 1
top level: BBa_E0035
direction: direction/forward
start: 81
end: 81
orientation: inline

A
type: SequenceAnnotation
persistent identity: annotation1959041
display id: annotation1959041
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959041/range1959041/1
role: feature/mutation
role: SO:0001059

https://synbiohub.org/public/igem/BBa_E0035/annotation1959041/range1959041/1
type: Range
persistent identity: range1959041
display id: range1959041
version: 1
top level: BBa_E0035
direction: direction/forward
start: 87
end: 87
orientation: inline

C
type: SequenceAnnotation
persistent identity: annotation1959042
display id: annotation1959042
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959042/range1959042/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_E0035/annotation1959042/range1959042/1
type: Range
persistent identity: range1959042
display id: range1959042
version: 1
top level: BBa_E0035
direction: direction/forward
start: 108
end: 108
orientation: inline

G
type: SequenceAnnotation
persistent identity: annotation1959043
display id: annotation1959043
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959043/range1959043/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_E0035/annotation1959043/range1959043/1
type: Range
persistent identity: range1959043
display id: range1959043
version: 1
top level: BBa_E0035
direction: direction/forward
start: 111
end: 111
orientation: inline

T7 promoter
type: SequenceAnnotation
persistent identity: annotation1959038
display id: annotation1959038
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959038/range1959038/1
role: feature/promoter
role: SO:0000167

https://synbiohub.org/public/igem/BBa_E0035/annotation1959038/range1959038/1
type: Range
persistent identity: range1959038
display id: range1959038
version: 1
top level: BBa_E0035
direction: direction/forward
start: 173
end: 191
orientation: inline

lacZ gene fragment
type: SequenceAnnotation
persistent identity: annotation1959044
display id: annotation1959044
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959044/range1959044/1
role: SO:0000110
role: feature/misc

https://synbiohub.org/public/igem/BBa_E0035/annotation1959044/range1959044/1
type: Range
persistent identity: range1959044
display id: range1959044
version: 1
top level: BBa_E0035
direction: direction/forward
start: 199
end: 348
orientation: inline

inserted T
type: SequenceAnnotation
persistent identity: annotation1959045
display id: annotation1959045
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959045/range1959045/1
role: SO:0001059
role: feature/mutation

https://synbiohub.org/public/igem/BBa_E0035/annotation1959045/range1959045/1
type: Range
persistent identity: range1959045
display id: range1959045
version: 1
top level: BBa_E0035
direction: direction/forward
start: 348
end: 348
orientation: inline

barcode
type: SequenceAnnotation
persistent identity: annotation1959046
display id: annotation1959046
version: 1
top level: BBa_E0035
location: https://synbiohub.org/public/igem/BBa_E0035/annotation1959046/range1959046/1
role: feature/barcode
role: SO:0000807

https://synbiohub.org/public/igem/BBa_E0035/annotation1959046/range1959046/1
type: Range
persistent identity: range1959046
display id: range1959046
version: 1
top level: BBa_E0035
direction: direction/forward
start: 355
end: 379
orientation: inline

https://synbiohub.org/public/igem/BBa_K077125_sequence/1
type: Sequence
persistent identity: BBa K077125 sequence
display id: BBa_K077125_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaatactagatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatcgctactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagagaaagaggagaaatactagatggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcg…
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0034_sequence/1
type: Sequence
persistent identity: BBa B0034 sequence
display id: BBa_B0034_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: aaagaggagaaa
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0012_sequence/1
type: Sequence
persistent identity: BBa B0012 sequence
display id: BBa_B0012_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tcacactggctcaccttcgggtgggcctttctgcgtttata
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_B0010_sequence/1
type: Sequence
persistent identity: BBa B0010 sequence
display id: BBa_B0010_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctc
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_R0040_sequence/1
type: Sequence
persistent identity: BBa R0040 sequence
display id: BBa_R0040_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0062_sequence/1
type: Sequence
persistent identity: BBa C0062 sequence
display id: BBa_C0062_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_C0079_sequence/1
type: Sequence
persistent identity: BBa C0079 sequence
display id: BBa_C0079_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atggccttggttgacggttttcttgagctggaacgctcaagtggaaaattggagtggagcgccatcctccagaagatggcgagcgaccttggattctcgaagatcctgttcggcctgttgcctaaggacagccaggactacgagaacgccttcatcgtcggcaactacccggccgcctggcgcgagcattacgaccgggctggctacgcgcgggtcgacccgacggtcagtcactgtacccagagcgtactgccgattttctgggaaccgtccatctaccagacgcgaaagcagcacgagttcttcgaggaagcctcggccgccggcctggtgtatgggctgaccatgccgctgcatggtgctcgcggcgaactcggcgcgctgagcctcagcgtggaagcggaaaaccgggccgaggccaaccgtttcatagagtcggtcctgccgaccctgtggatgctcaaggactacgcactgcaaagcggtgccggactggccttcgaacatccggtcagcaaaccggtggttctgaccagccgggagaaggaagtgttgcagtggtgcgccatcggcaagaccagttgggagatatcggttatctgcaactgctcggaagccaatgtgaacttccatatgggaaatattcggcggaagttcggtgtgacctcccgccgcgtagcggccattatggccgttaatttgggtcttattactctcgctgcaaacgacgaaaactacgctttagtagcttaataacactgatagtgctagtgtagatcac
encoding: naseq.html

https://synbiohub.org/public/igem/BBa_E0035_sequence/1
type: Sequence
persistent identity: BBa E0035 sequence
display id: BBa_E0035_sequence
version: 1
was generated by: iGEM to SBOL conversion
owned by: james
owned by: myers
elements: atgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggcagcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggcccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaaaccctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcaccgatcgcccttcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatcgc
encoding: naseq.html

iGEM to SBOL conversion
type: Activity
persistent identity: igem2sbol
display id: igem2sbol
version: 1
description: Conversion of the iGEM parts registry to SBOL2.1
owned by: james
owned by: myers
creator: James Alastair McLaughlin
creator: Chris J. Myers
ended at time: 2017-03-06T15:00:00.000Z