LOCUS       BBa_K077126             1603 bp    DNA     linear       22-Nov-2024
DEFINITION  Test construct for HSL/LuxR promotor, with LacZalpha output
ACCESSION   BBa_K077126
VERSION     BBa_K077126.1  
FEATURES             Location/Qualifiers
     misc_feature    1..20
                     /label=LuxR/HSL
     promoter        20..25
                     /label=-35
     promoter        42..47
                     /label=-10
     misc_feature    53..53
                     /label=start
     misc_feature    1..55
                     /label=BBa_R0062
     promoter        1..55
                     /label=BBa_R0062
     misc_feature    68..71
                     /label=conserved
     RBS             64..75
                     /label=BBa_B0034
     misc_feature    82..96
                     /label=lacZ gene fragment
     CDS             82..435
                     /label=lacZ alpha
     promoter        107..116
                     /label=T3 promoter
     promoter        254..272
                     /label=T7 promoter
     misc_feature    159..159
                     /label=C
     misc_feature    162..162
                     /label=T
     misc_feature    168..168
                     /label=A
     misc_feature    189..189
                     /label=C
     misc_feature    192..192
                     /label=G
     misc_feature    280..429
                     /label=lacZ gene fragment
     misc_feature    429..429
                     /label=inserted T
     misc_feature    436..460
                     /label=barcode
     misc_feature    82..460
                     /label=BBa_E0035
     stem_loop       480..523
                     /label=stem_loop
     misc_feature    469..548
                     /label=BBa_B0010
     terminator      469..548
                     /label=BBa_B0010
     stem_loop       564..583
                     /label=T7 TE
     misc_feature    590..590
                     /label=stop
     polyA_site      584..597
                     /label=polya
     misc_feature    557..597
                     /label=BBa_B0012
     terminator      557..597
                     /label=BBa_B0012
     terminator      469..597
                     /label=BBa_B0015
     misc_feature    64..597
                     /label=BBa_E0435
     misc_feature    606..624
                     /label=TetR 1
     misc_feature    606..659
                     /label=BBa_R0040
     promoter        625..630
                     /label=-35
     misc_feature    631..649
                     /label=TetR 2
     promoter        648..653
                     /label=-10
     promoter        606..659
                     /label=BBa_R0040
     misc_feature    672..675
                     /label=conserved
     RBS             668..679
                     /label=BBa_B0034
     misc_feature    686..687
                     /label=prefix
     misc_feature    859..859
                     /label=T
     misc_feature    1177..1177
                     /label=A
     CDS             686..1435
                     /label=luxR
     misc_feature    686..1441
                     /label=BBa_C0062
     misc_feature    1442..1466
                     /label=Help:Barcodes
     CDS             686..1441
                     /label=BBa_C0062
     stem_loop       1486..1529
                     /label=stem_loop
     misc_feature    1475..1554
                     /label=BBa_B0010
     terminator      1475..1554
                     /label=BBa_B0010
     stem_loop       1570..1589
                     /label=T7 TE
     misc_feature    1596..1596
                     /label=stop
     polyA_site      1590..1603
                     /label=polya
     misc_feature    1563..1603
                     /label=BBa_B0012
     terminator      1563..1603
                     /label=BBa_B0012
     misc_feature    606..1603
                     /label=BBa_S03119
     misc_feature    64..1603
                     /label=BBa_K077124
ORIGIN
        1 acctgtagga tcgtacaggt ttacgcaaga aaatggtttg ttatagtcga ataaatacta
       61 gagaaagagg agaaatacta gatgaccatg attacgccaa gcgcgcaatt aaccctcact
      121 aaagggaaca aaagctggag ctccaccgcg gtggcggcag cactagagct agtggatccc
      181 ccgggctgta gaaattcgat atcaagctta tcgataccgt cgacctcgag ggggggcccg
      241 gtacccaatt cgccctatag tgagtcgtat tacgcgcgct cactggccgt cgttttacaa
      301 cgtcgtgact gggaaaaccc tggcgttacc caacttaatc gccttgcagc acatccccct
      361 ttcgccagct ggcgtaatag cgaagaggcc cgcaccgatc gcccttccca acagttgcgc
      421 agcctgaatt aataacgctg atagtgctag tgtagatcgc tactagagcc aggcatcaaa
      481 taaaacgaaa ggctcagtcg aaagactggg cctttcgttt tatctgttgt ttgtcggtga
      541 acgctctcta ctagagtcac actggctcac cttcgggtgg gcctttctgc gtttatatac
      601 tagagtccct atcagtgata gagattgaca tccctatcag tgatagagat actgagcact
      661 actagagaaa gaggagaaat actagatgaa aaacataaat gccgacgaca catacagaat
      721 aattaataaa attaaagctt gtagaagcaa taatgatatt aatcaatgct tatctgatat
      781 gactaaaatg gtacattgtg aatattattt actcgcgatc atttatcctc attctatggt
      841 taaatctgat atttcaatcc tagataatta ccctaaaaaa tggaggcaat attatgatga
      901 cgctaattta ataaaatatg atcctatagt agattattct aactccaatc attcaccaat
      961 taattggaat atatttgaaa acaatgctgt aaataaaaaa tctccaaatg taattaaaga
     1021 agcgaaaaca tcaggtctta tcactgggtt tagtttccct attcatacgg ctaacaatgg
     1081 cttcggaatg cttagttttg cacattcaga aaaagacaac tatatagata gtttattttt
     1141 acatgcgtgt atgaacatac cattaattgt tccttctcta gttgataatt atcgaaaaat
     1201 aaatatagca aataataaat caaacaacga tttaaccaaa agagaaaaag aatgtttagc
     1261 gtgggcatgc gaaggaaaaa gctcttggga tatttcaaaa atattaggtt gcagtgagcg
     1321 tactgtcact ttccatttaa ccaatgcgca aatgaaactc aatacaacaa accgctgcca
     1381 aagtatttct aaagcaattt taacaggagc aattgattgc ccatacttta aaaattaata
     1441 acactgatag tgctagtgta gatcactact agagccaggc atcaaataaa acgaaaggct
     1501 cagtcgaaag actgggcctt tcgttttatc tgttgtttgt cggtgaacgc tctctactag
     1561 agtcacactg gctcaccttc gggtgggcct ttctgcgttt ata
//