##gff-version 3
##sequence-region BBa_K077126 1 1603
BBa_K077126	.	engineered_region	64	1603	.	+	0	ID=BBa_K077124;Name=BBa_K077124
BBa_K077126	.	engineered_region	64	597	.	+	0	ID=BBa_E0435;Name=BBa_E0435;Parent=BBa_K077124
BBa_K077126	.	terminator	469	597	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_E0435
BBa_K077126	.	terminator	469	548	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K077126	.	engineered_region	469	548	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K077126	.	stem_loop	480	523	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K077126	.	terminator	557	597	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K077126	.	polyA_site	584	597	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K077126	.	engineered_region	557	597	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K077126	.	stop_codon	590	590	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K077126	.	stem_loop	564	583	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K077126	.	ribosome_entry_site	64	75	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_E0435
BBa_K077126	.	conserved_region	68	71	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K077126	.	engineered_region	82	460	.	+	0	ID=BBa_E0035;Name=BBa_E0035;Parent=BBa_E0435
BBa_K077126	.	promoter	254	272	.	+	0	ID=annotation1959038;Name=T7 promoter;Parent=BBa_E0035
BBa_K077126	.	sequence_alteration	189	189	.	+	0	ID=annotation1959042;Name=C;Parent=BBa_E0035
BBa_K077126	.	CDS	82	435	.	+	0	ID=annotation1959036;Name=lacZ alpha;Parent=BBa_E0035
BBa_K077126	.	sequence_alteration	162	162	.	+	0	ID=annotation1959040;Name=T;Parent=BBa_E0035
BBa_K077126	.	promoter	107	116	.	+	0	ID=annotation1959037;Name=T3 promoter;Parent=BBa_E0035
BBa_K077126	.	sequence_alteration	429	429	.	+	0	ID=annotation1959045;Name=inserted T;Parent=BBa_E0035
BBa_K077126	.	sequence_feature	82	96	.	+	0	ID=annotation1959035;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_K077126	.	engineered_tag	436	460	.	+	0	ID=annotation1959046;Name=barcode;Parent=BBa_E0035
BBa_K077126	.	sequence_alteration	192	192	.	+	0	ID=annotation1959043;Name=G;Parent=BBa_E0035
BBa_K077126	.	sequence_alteration	168	168	.	+	0	ID=annotation1959041;Name=A;Parent=BBa_E0035
BBa_K077126	.	sequence_alteration	159	159	.	+	0	ID=annotation1959039;Name=C;Parent=BBa_E0035
BBa_K077126	.	sequence_feature	280	429	.	+	0	ID=annotation1959044;Name=lacZ gene fragment;Parent=BBa_E0035
BBa_K077126	.	engineered_region	606	1603	.	+	0	ID=BBa_S03119;Name=BBa_S03119;Parent=BBa_K077124
BBa_K077126	.	terminator	1475	1554	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03119
BBa_K077126	.	engineered_region	1475	1554	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K077126	.	stem_loop	1486	1529	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K077126	.	terminator	1563	1603	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03119
BBa_K077126	.	polyA_site	1590	1603	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K077126	.	engineered_region	1563	1603	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K077126	.	stop_codon	1596	1596	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K077126	.	stem_loop	1570	1589	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K077126	.	promoter	606	659	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_S03119
BBa_K077126	.	promoter	625	630	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K077126	.	engineered_region	606	659	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K077126	.	non_covalent_binding_site	606	624	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K077126	.	promoter	648	653	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K077126	.	non_covalent_binding_site	631	649	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K077126	.	CDS	686	1441	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S03119
BBa_K077126	.	engineered_region	686	1441	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K077126	.	CDS	686	1435	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K077126	.	point_mutation	859	859	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K077126	.	engineered_tag	1442	1466	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K077126	.	engineered_region	686	687	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K077126	.	point_mutation	1177	1177	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K077126	.	ribosome_entry_site	668	679	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03119
BBa_K077126	.	conserved_region	672	675	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K077126	.	promoter	1	55	.	+	0	ID=BBa_R0062;Name=BBa_R0062
BBa_K077126	.	promoter	20	25	.	+	0	ID=annotation2046;Name=-35;Parent=BBa_R0062
BBa_K077126	.	start_codon	53	53	.	+	0	ID=annotation2048;Name=start;Parent=BBa_R0062
BBa_K077126	.	promoter	42	47	.	+	0	ID=annotation2047;Name=-10;Parent=BBa_R0062
BBa_K077126	.	engineered_region	1	55	.	+	0	ID=annotation7070;Name=BBa_R0062;Parent=BBa_R0062
BBa_K077126	.	non_covalent_binding_site	1	20	.	+	0	ID=annotation2045;Name=LuxR/HSL;Parent=BBa_R0062
>BBa_K077126
acctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataaatactagagaaagaggagaaatact
agatgaccatgattacgccaagcgcgcaattaaccctcactaaagggaacaaaagctggagctccaccgcggtggcggc
agcactagagctagtggatcccccgggctgtagaaattcgatatcaagcttatcgataccgtcgacctcgagggggggc
ccggtacccaattcgccctatagtgagtcgtattacgcgcgctcactggccgtcgttttacaacgtcgtgactgggaaa
accctggcgttacccaacttaatcgccttgcagcacatccccctttcgccagctggcgtaatagcgaagaggcccgcac
cgatcgcccttcccaacagttgcgcagcctgaattaataacgctgatagtgctagtgtagatcgctactagagccaggc
atcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctacta
gagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatc
cctatcagtgatagagatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacac
atacagaataattaataaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatg
gtacattgtgaatattatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataatt
accctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaa
tcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaa
acatcaggtcttatcactgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacatt
cagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttga
taattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgg
gcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaacca
atgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgccc
atactttaaaaattaataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctc
agtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttc
gggtgggcctttctgcgtttata