LOCUS BBa_K077556 2694 bp DNA linear 22-Nov-2024 DEFINITION K077555 plus LuxR ACCESSION BBa_K077556 VERSION BBa_K077556.1 FEATURES Location/Qualifiers misc_feature 1..97 /label=BBa_R0065 misc_feature 6..25 /label=Lux Box promoter 47..52 /label=-10 misc_feature 57..73 /label=OR2 cI promoter 58..58 /label=lux p(R) start promoter 71..76 /label=-35 misc_feature 81..97 /label=OR1 cI promoter 94..97 /label=-10 promoter 1..97 /label=BBa_R0065 misc_feature 110..113 /label=conserved RBS 106..117 /label=BBa_B0034 misc_feature 703..734 /label=LVA CDS 124..702 /label=luxI misc_feature 124..741 /label=BBa_C0061 misc_feature 742..766 /label=Help:Barcodes CDS 124..741 /label=BBa_C0061 misc_feature 779..782 /label=conserved RBS 775..786 /label=BBa_B0034 misc_feature 793..1512 /label=BBa_E0040 misc_feature 1507..1545 /label=LVA misc_feature 1546..1551 /label=Double Stop misc_feature 793..795 /label=Start misc_feature 793..1551 /label=BBa_J04031 stem_loop 1571..1614 /label=stem_loop misc_feature 1560..1639 /label=BBa_B0010 terminator 1560..1639 /label=BBa_B0010 stem_loop 1655..1674 /label=T7 TE misc_feature 1681..1681 /label=stop polyA_site 1675..1688 /label=polya misc_feature 1648..1688 /label=BBa_B0012 terminator 1648..1688 /label=BBa_B0012 terminator 1560..1688 /label=BBa_B0015 misc_feature 1..1688 /label=BBa_K077555 misc_feature 1697..1715 /label=TetR 1 misc_feature 1697..1750 /label=BBa_R0040 promoter 1716..1721 /label=-35 misc_feature 1722..1740 /label=TetR 2 promoter 1739..1744 /label=-10 promoter 1697..1750 /label=BBa_R0040 misc_feature 1763..1766 /label=conserved RBS 1759..1770 /label=BBa_B0034 misc_feature 1777..1778 /label=prefix misc_feature 1950..1950 /label=T misc_feature 2268..2268 /label=A CDS 1777..2526 /label=luxR misc_feature 1777..2532 /label=BBa_C0062 misc_feature 2533..2557 /label=Help:Barcodes CDS 1777..2532 /label=BBa_C0062 stem_loop 2577..2620 /label=stem_loop misc_feature 2566..2645 /label=BBa_B0010 terminator 2566..2645 /label=BBa_B0010 stem_loop 2661..2680 /label=T7 TE misc_feature 2687..2687 /label=stop polyA_site 2681..2694 /label=polya misc_feature 2654..2694 /label=BBa_B0012 terminator 2654..2694 /label=BBa_B0012 misc_feature 1697..2694 /label=BBa_S03119 ORIGIN 1 taagcacctg taggatcgta caggtttacg caagaaaatg gtttgttata gtcgaataac 61 accgtgcgtg ttgactattt tacctctggc ggtgatatac tagagaaaga ggagaaatac 121 tagatgacta taatgataaa aaaatcggat tttttggcaa ttccatcgga ggagtataaa 181 ggtattctaa gtcttcgtta tcaagtgttt aagcaaagac ttgagtggga cttagttgta 241 gaaaataacc ttgaatcaga tgagtatgat aactcaaatg cagaatatat ttatgcttgt 301 gatgatactg aaaatgtaag tggatgctgg cgtttattac ctacaacagg tgattatatg 361 ctgaaaagtg tttttcctga attgcttggt caacagagtg ctcccaaaga tcctaatata 421 gtcgaattaa gtcgttttgc tgtaggtaaa aatagctcaa agataaataa ctctgctagt 481 gaaattacaa tgaaactatt tgaagctata tataaacacg ctgttagtca aggtattaca 541 gaatatgtaa cagtaacatc aacagcaata gagcgatttt taaagcgtat taaagttcct 601 tgtcatcgta ttggagacaa agaaattcat gtattaggtg atactaaatc ggttgtattg 661 tctatgccta ttaatgaaca gtttaaaaaa gcagtcttaa atgctgcaaa cgacgaaaac 721 tacgctttag tagcttaata actctgatag tgctagtgta gatctctact agagaaagag 781 gagaaatact agatgcgtaa aggagaagaa cttttcactg gagttgtccc aattcttgtt 841 gaattagatg gtgatgttaa tgggcacaaa ttttctgtca gtggagaggg tgaaggtgat 901 gcaacatacg gaaaacttac ccttaaattt atttgcacta ctggaaaact acctgttcca 961 tggccaacac ttgtcactac tttcggttat ggtgttcaat gctttgcgag atacccagat 1021 catatgaaac agcatgactt tttcaagagt gccatgcccg aaggttatgt acaggaaaga 1081 actatatttt tcaaagatga cgggaactac aagacacgtg ctgaagtcaa gtttgaaggt 1141 gatacccttg ttaatagaat cgagttaaaa ggtattgatt ttaaagaaga tggaaacatt 1201 cttggacaca aattggaata caactataac tcacacaatg tatacatcat ggcagacaaa 1261 caaaagaatg gaatcaaagt taacttcaaa attagacaca acattgaaga tggaagcgtt 1321 caactagcag accattatca acaaaatact ccaattggcg atggccctgt ccttttacca 1381 gacaaccatt acctgtccac acaatctgcc ctttcgaaag atcccaacga aaagagagac 1441 cacatggtcc ttcttgagtt tgtaacagct gctgggatta cacatggcat ggatgaacta 1501 tacaaaaggc ctgctgcaaa cgacgaaaac tacgctttag tagcttaata atactagagc 1561 caggcatcaa ataaaacgaa aggctcagtc gaaagactgg gcctttcgtt ttatctgttg 1621 tttgtcggtg aacgctctct actagagtca cactggctca ccttcgggtg ggcctttctg 1681 cgtttatata ctagagtccc tatcagtgat agagattgac atccctatca gtgatagaga 1741 tactgagcac tactagagaa agaggagaaa tactagatga aaaacataaa tgccgacgac 1801 acatacagaa taattaataa aattaaagct tgtagaagca ataatgatat taatcaatgc 1861 ttatctgata tgactaaaat ggtacattgt gaatattatt tactcgcgat catttatcct 1921 cattctatgg ttaaatctga tatttcaatc ctagataatt accctaaaaa atggaggcaa 1981 tattatgatg acgctaattt aataaaatat gatcctatag tagattattc taactccaat 2041 cattcaccaa ttaattggaa tatatttgaa aacaatgctg taaataaaaa atctccaaat 2101 gtaattaaag aagcgaaaac atcaggtctt atcactgggt ttagtttccc tattcatacg 2161 gctaacaatg gcttcggaat gcttagtttt gcacattcag aaaaagacaa ctatatagat 2221 agtttatttt tacatgcgtg tatgaacata ccattaattg ttccttctct agttgataat 2281 tatcgaaaaa taaatatagc aaataataaa tcaaacaacg atttaaccaa aagagaaaaa 2341 gaatgtttag cgtgggcatg cgaaggaaaa agctcttggg atatttcaaa aatattaggt 2401 tgcagtgagc gtactgtcac tttccattta accaatgcgc aaatgaaact caatacaaca 2461 aaccgctgcc aaagtatttc taaagcaatt ttaacaggag caattgattg cccatacttt 2521 aaaaattaat aacactgata gtgctagtgt agatcactac tagagccagg catcaaataa 2581 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 2641 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //