##gff-version 3
##sequence-region BBa_K077556 1 2694
BBa_K077556	.	engineered_region	1697	2694	.	+	0	ID=BBa_S03119;Name=BBa_S03119
BBa_K077556	.	terminator	2654	2694	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03119
BBa_K077556	.	stop_codon	2687	2687	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K077556	.	polyA_site	2681	2694	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K077556	.	stem_loop	2661	2680	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K077556	.	engineered_region	2654	2694	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K077556	.	ribosome_entry_site	1759	1770	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03119
BBa_K077556	.	conserved_region	1763	1766	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K077556	.	promoter	1697	1750	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_S03119
BBa_K077556	.	engineered_region	1697	1750	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K077556	.	promoter	1716	1721	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K077556	.	promoter	1739	1744	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K077556	.	non_covalent_binding_site	1697	1715	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K077556	.	non_covalent_binding_site	1722	1740	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K077556	.	CDS	1777	2532	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S03119
BBa_K077556	.	engineered_tag	2533	2557	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K077556	.	engineered_region	1777	2532	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K077556	.	engineered_region	1777	1778	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K077556	.	point_mutation	1950	1950	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K077556	.	CDS	1777	2526	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K077556	.	point_mutation	2268	2268	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K077556	.	terminator	2566	2645	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03119
BBa_K077556	.	engineered_region	2566	2645	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K077556	.	stem_loop	2577	2620	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K077556	.	engineered_region	1	1688	.	+	0	ID=BBa_K077555;Name=BBa_K077555
BBa_K077556	.	ribosome_entry_site	106	117	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K077555
BBa_K077556	.	conserved_region	110	113	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K077556	.	terminator	1560	1688	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K077555
BBa_K077556	.	terminator	1560	1639	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K077556	.	engineered_region	1560	1639	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K077556	.	stem_loop	1571	1614	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K077556	.	terminator	1648	1688	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K077556	.	stop_codon	1681	1681	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K077556	.	polyA_site	1675	1688	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K077556	.	stem_loop	1655	1674	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K077556	.	engineered_region	1648	1688	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K077556	.	promoter	1	97	.	+	0	ID=BBa_R0065;Name=BBa_R0065;Parent=BBa_K077555
BBa_K077556	.	promoter	71	76	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_K077556	.	non_covalent_binding_site	57	73	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_K077556	.	promoter	58	58	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_K077556	.	non_covalent_binding_site	6	25	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_K077556	.	engineered_region	1	97	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_K077556	.	non_covalent_binding_site	81	97	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_K077556	.	promoter	47	52	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_K077556	.	promoter	94	97	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_K077556	.	engineered_region	793	1551	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_K077555
BBa_K077556	.	stop_codon	1546	1551	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_K077556	.	start_codon	793	795	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_K077556	.	tag	1507	1545	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_K077556	.	engineered_region	793	1512	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_K077556	.	CDS	124	741	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K077555
BBa_K077556	.	CDS	124	702	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K077556	.	engineered_tag	742	766	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K077556	.	engineered_region	124	741	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K077556	.	tag	703	734	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K077556	.	ribosome_entry_site	775	786	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K077555
BBa_K077556	.	conserved_region	779	782	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
>BBa_K077556
taagcacctgtaggatcgtacaggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactatt
ttacctctggcggtgatatactagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggc
aattccatcggaggagtataaaggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagtt
gtagaaaataaccttgaatcagatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatg
taagtggatgctggcgtttattacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaaca
gagtgctcccaaagatcctaatatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactct
gctagtgaaattacaatgaaactatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacag
taacatcaacagcaatagagcgatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgt
attaggtgatactaaatcggttgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaac
gacgaaaactacgctttagtagcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatact
agatgcgtaaaggagaagaacttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaa
attttctgtcagtggagagggtgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaa
ctacctgttccatggccaacacttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatga
aacagcatgactttttcaagagtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaa
ctacaagacacgtgctgaagtcaagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaa
gaagatggaaacattcttggacacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaa
agaatggaatcaaagttaacttcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaaca
aaatactccaattggcgatggccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagat
cccaacgaaaagagagaccacatggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactat
acaaaaggcctgctgcaaacgacgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaa
aggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctc
accttcgggtgggcctttctgcgtttatatactagagtccctatcagtgatagagattgacatccctatcagtgataga
gatactgagcactactagagaaagaggagaaatactagatgaaaaacataaatgccgacgacacatacagaataattaa
taaaattaaagcttgtagaagcaataatgatattaatcaatgcttatctgatatgactaaaatggtacattgtgaatat
tatttactcgcgatcatttatcctcattctatggttaaatctgatatttcaatcctagataattaccctaaaaaatgga
ggcaatattatgatgacgctaatttaataaaatatgatcctatagtagattattctaactccaatcattcaccaattaa
ttggaatatatttgaaaacaatgctgtaaataaaaaatctccaaatgtaattaaagaagcgaaaacatcaggtcttatc
actgggtttagtttccctattcatacggctaacaatggcttcggaatgcttagttttgcacattcagaaaaagacaact
atatagatagtttatttttacatgcgtgtatgaacataccattaattgttccttctctagttgataattatcgaaaaat
aaatatagcaaataataaatcaaacaacgatttaaccaaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaa
agctcttgggatatttcaaaaatattaggttgcagtgagcgtactgtcactttccatttaaccaatgcgcaaatgaaac
tcaatacaacaaaccgctgccaaagtatttctaaagcaattttaacaggagcaattgattgcccatactttaaaaatta
ataacactgatagtgctagtgtagatcactactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttata