LOCUS BBa_K077557 2694 bp DNA linear 22-Dec-2024 DEFINITION Part 1 of the Conways game of life system ACCESSION BBa_K077557 VERSION BBa_K077557.1 FEATURES Location/Qualifiers misc_feature 1..19 /label=TetR 1 misc_feature 1..54 /label=BBa_R0040 promoter 20..25 /label=-35 misc_feature 26..44 /label=TetR 2 promoter 43..48 /label=-10 promoter 1..54 /label=BBa_R0040 misc_feature 67..70 /label=conserved RBS 63..74 /label=BBa_B0034 misc_feature 81..82 /label=prefix misc_feature 254..254 /label=T misc_feature 572..572 /label=A CDS 81..830 /label=luxR misc_feature 81..836 /label=BBa_C0062 misc_feature 837..861 /label=Help:Barcodes CDS 81..836 /label=BBa_C0062 stem_loop 881..924 /label=stem_loop misc_feature 870..949 /label=BBa_B0010 terminator 870..949 /label=BBa_B0010 stem_loop 965..984 /label=T7 TE misc_feature 991..991 /label=stop polyA_site 985..998 /label=polya misc_feature 958..998 /label=BBa_B0012 terminator 958..998 /label=BBa_B0012 misc_feature 1..998 /label=BBa_S03119 misc_feature 1007..1103 /label=BBa_R0065 misc_feature 1012..1031 /label=Lux Box promoter 1053..1058 /label=-10 misc_feature 1063..1079 /label=OR2 cI promoter 1064..1064 /label=lux p(R) start promoter 1077..1082 /label=-35 misc_feature 1087..1103 /label=OR1 cI promoter 1100..1103 /label=-10 promoter 1007..1103 /label=BBa_R0065 misc_feature 1116..1119 /label=conserved RBS 1112..1123 /label=BBa_B0034 misc_feature 1709..1740 /label=LVA CDS 1130..1708 /label=luxI misc_feature 1130..1747 /label=BBa_C0061 misc_feature 1748..1772 /label=Help:Barcodes CDS 1130..1747 /label=BBa_C0061 misc_feature 1785..1788 /label=conserved RBS 1781..1792 /label=BBa_B0034 misc_feature 1799..2518 /label=BBa_E0040 misc_feature 2513..2551 /label=LVA misc_feature 2552..2557 /label=Double Stop misc_feature 1799..1801 /label=Start misc_feature 1799..2557 /label=BBa_J04031 stem_loop 2577..2620 /label=stem_loop misc_feature 2566..2645 /label=BBa_B0010 terminator 2566..2645 /label=BBa_B0010 stem_loop 2661..2680 /label=T7 TE misc_feature 2687..2687 /label=stop polyA_site 2681..2694 /label=polya misc_feature 2654..2694 /label=BBa_B0012 terminator 2654..2694 /label=BBa_B0012 terminator 2566..2694 /label=BBa_B0015 misc_feature 1007..2694 /label=BBa_K077555 ORIGIN 1 tccctatcag tgatagagat tgacatccct atcagtgata gagatactga gcactactag 61 agaaagagga gaaatactag atgaaaaaca taaatgccga cgacacatac agaataatta 121 ataaaattaa agcttgtaga agcaataatg atattaatca atgcttatct gatatgacta 181 aaatggtaca ttgtgaatat tatttactcg cgatcattta tcctcattct atggttaaat 241 ctgatatttc aatcctagat aattacccta aaaaatggag gcaatattat gatgacgcta 301 atttaataaa atatgatcct atagtagatt attctaactc caatcattca ccaattaatt 361 ggaatatatt tgaaaacaat gctgtaaata aaaaatctcc aaatgtaatt aaagaagcga 421 aaacatcagg tcttatcact gggtttagtt tccctattca tacggctaac aatggcttcg 481 gaatgcttag ttttgcacat tcagaaaaag acaactatat agatagttta tttttacatg 541 cgtgtatgaa cataccatta attgttcctt ctctagttga taattatcga aaaataaata 601 tagcaaataa taaatcaaac aacgatttaa ccaaaagaga aaaagaatgt ttagcgtggg 661 catgcgaagg aaaaagctct tgggatattt caaaaatatt aggttgcagt gagcgtactg 721 tcactttcca tttaaccaat gcgcaaatga aactcaatac aacaaaccgc tgccaaagta 781 tttctaaagc aattttaaca ggagcaattg attgcccata ctttaaaaat taataacact 841 gatagtgcta gtgtagatca ctactagagc caggcatcaa ataaaacgaa aggctcagtc 901 gaaagactgg gcctttcgtt ttatctgttg tttgtcggtg aacgctctct actagagtca 961 cactggctca ccttcgggtg ggcctttctg cgtttatata ctagagtaag cacctgtagg 1021 atcgtacagg tttacgcaag aaaatggttt gttatagtcg aataacaccg tgcgtgttga 1081 ctattttacc tctggcggtg atatactaga gaaagaggag aaatactaga tgactataat 1141 gataaaaaaa tcggattttt tggcaattcc atcggaggag tataaaggta ttctaagtct 1201 tcgttatcaa gtgtttaagc aaagacttga gtgggactta gttgtagaaa ataaccttga 1261 atcagatgag tatgataact caaatgcaga atatatttat gcttgtgatg atactgaaaa 1321 tgtaagtgga tgctggcgtt tattacctac aacaggtgat tatatgctga aaagtgtttt 1381 tcctgaattg cttggtcaac agagtgctcc caaagatcct aatatagtcg aattaagtcg 1441 ttttgctgta ggtaaaaata gctcaaagat aaataactct gctagtgaaa ttacaatgaa 1501 actatttgaa gctatatata aacacgctgt tagtcaaggt attacagaat atgtaacagt 1561 aacatcaaca gcaatagagc gatttttaaa gcgtattaaa gttccttgtc atcgtattgg 1621 agacaaagaa attcatgtat taggtgatac taaatcggtt gtattgtcta tgcctattaa 1681 tgaacagttt aaaaaagcag tcttaaatgc tgcaaacgac gaaaactacg ctttagtagc 1741 ttaataactc tgatagtgct agtgtagatc tctactagag aaagaggaga aatactagat 1801 gcgtaaagga gaagaacttt tcactggagt tgtcccaatt cttgttgaat tagatggtga 1861 tgttaatggg cacaaatttt ctgtcagtgg agagggtgaa ggtgatgcaa catacggaaa 1921 acttaccctt aaatttattt gcactactgg aaaactacct gttccatggc caacacttgt 1981 cactactttc ggttatggtg ttcaatgctt tgcgagatac ccagatcata tgaaacagca 2041 tgactttttc aagagtgcca tgcccgaagg ttatgtacag gaaagaacta tatttttcaa 2101 agatgacggg aactacaaga cacgtgctga agtcaagttt gaaggtgata cccttgttaa 2161 tagaatcgag ttaaaaggta ttgattttaa agaagatgga aacattcttg gacacaaatt 2221 ggaatacaac tataactcac acaatgtata catcatggca gacaaacaaa agaatggaat 2281 caaagttaac ttcaaaatta gacacaacat tgaagatgga agcgttcaac tagcagacca 2341 ttatcaacaa aatactccaa ttggcgatgg ccctgtcctt ttaccagaca accattacct 2401 gtccacacaa tctgcccttt cgaaagatcc caacgaaaag agagaccaca tggtccttct 2461 tgagtttgta acagctgctg ggattacaca tggcatggat gaactataca aaaggcctgc 2521 tgcaaacgac gaaaactacg ctttagtagc ttaataatac tagagccagg catcaaataa 2581 aacgaaaggc tcagtcgaaa gactgggcct ttcgttttat ctgttgtttg tcggtgaacg 2641 ctctctacta gagtcacact ggctcacctt cgggtgggcc tttctgcgtt tata //