##gff-version 3
##sequence-region BBa_K077557 1 2694
BBa_K077557	.	engineered_region	1007	2694	.	+	0	ID=BBa_K077555;Name=BBa_K077555
BBa_K077557	.	promoter	1007	1103	.	+	0	ID=BBa_R0065;Name=BBa_R0065;Parent=BBa_K077555
BBa_K077557	.	engineered_region	1007	1103	.	+	0	ID=annotation1986774;Name=BBa_R0065;Parent=BBa_R0065
BBa_K077557	.	non_covalent_binding_site	1087	1103	.	+	0	ID=annotation1986780;Name=OR1 cI;Parent=BBa_R0065
BBa_K077557	.	promoter	1053	1058	.	+	0	ID=annotation1986776;Name=-10;Parent=BBa_R0065
BBa_K077557	.	non_covalent_binding_site	1063	1079	.	+	0	ID=annotation1986777;Name=OR2 cI;Parent=BBa_R0065
BBa_K077557	.	promoter	1077	1082	.	+	0	ID=annotation1986779;Name=-35;Parent=BBa_R0065
BBa_K077557	.	promoter	1064	1064	.	+	0	ID=annotation1986778;Name=lux p(R) start;Parent=BBa_R0065
BBa_K077557	.	non_covalent_binding_site	1012	1031	.	+	0	ID=annotation1986775;Name=Lux Box;Parent=BBa_R0065
BBa_K077557	.	promoter	1100	1103	.	+	0	ID=annotation1986781;Name=-10;Parent=BBa_R0065
BBa_K077557	.	engineered_region	1799	2557	.	+	0	ID=BBa_J04031;Name=BBa_J04031;Parent=BBa_K077555
BBa_K077557	.	tag	2513	2551	.	+	0	ID=annotation1507235;Name=LVA;Parent=BBa_J04031
BBa_K077557	.	engineered_region	1799	2518	.	+	0	ID=annotation1507202;Name=BBa_E0040;Parent=BBa_J04031
BBa_K077557	.	start_codon	1799	1801	.	+	0	ID=annotation1507246;Name=Start;Parent=BBa_J04031
BBa_K077557	.	stop_codon	2552	2557	.	+	0	ID=annotation1507242;Name=Double Stop;Parent=BBa_J04031
BBa_K077557	.	CDS	1130	1747	.	+	0	ID=BBa_C0061;Name=BBa_C0061;Parent=BBa_K077555
BBa_K077557	.	CDS	1130	1708	.	+	0	ID=annotation1761;Name=luxI;Parent=BBa_C0061
BBa_K077557	.	engineered_tag	1748	1772	.	+	0	ID=annotation2213985;Name=Help:Barcodes;Parent=BBa_C0061
BBa_K077557	.	engineered_region	1130	1747	.	+	0	ID=annotation7038;Name=BBa_C0061;Parent=BBa_C0061
BBa_K077557	.	tag	1709	1740	.	+	0	ID=annotation1760;Name=LVA;Parent=BBa_C0061
BBa_K077557	.	terminator	2566	2694	.	+	0	ID=BBa_B0015;Name=BBa_B0015;Parent=BBa_K077555
BBa_K077557	.	terminator	2654	2694	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_B0015
BBa_K077557	.	polyA_site	2681	2694	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K077557	.	stem_loop	2661	2680	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K077557	.	engineered_region	2654	2694	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K077557	.	stop_codon	2687	2687	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K077557	.	terminator	2566	2645	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_B0015
BBa_K077557	.	stem_loop	2577	2620	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K077557	.	engineered_region	2566	2645	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
BBa_K077557	.	ribosome_entry_site	1112	1123	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K077555
BBa_K077557	.	conserved_region	1116	1119	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K077557	.	ribosome_entry_site	1781	1792	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_K077555
BBa_K077557	.	conserved_region	1785	1788	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K077557	.	engineered_region	1	998	.	+	0	ID=BBa_S03119;Name=BBa_S03119
BBa_K077557	.	CDS	81	836	.	+	0	ID=BBa_C0062;Name=BBa_C0062;Parent=BBa_S03119
BBa_K077557	.	point_mutation	572	572	.	+	0	ID=annotation1765;Name=A;Parent=BBa_C0062
BBa_K077557	.	CDS	81	830	.	+	0	ID=annotation1766;Name=luxR;Parent=BBa_C0062
BBa_K077557	.	point_mutation	254	254	.	+	0	ID=annotation1764;Name=T;Parent=BBa_C0062
BBa_K077557	.	engineered_tag	837	861	.	+	0	ID=annotation2213986;Name=Help:Barcodes;Parent=BBa_C0062
BBa_K077557	.	engineered_region	81	836	.	+	0	ID=annotation7039;Name=BBa_C0062;Parent=BBa_C0062
BBa_K077557	.	engineered_region	81	82	.	+	0	ID=annotation1762;Name=prefix;Parent=BBa_C0062
BBa_K077557	.	terminator	958	998	.	+	0	ID=BBa_B0012;Name=BBa_B0012;Parent=BBa_S03119
BBa_K077557	.	polyA_site	985	998	.	+	0	ID=annotation1690;Name=polya;Parent=BBa_B0012
BBa_K077557	.	stem_loop	965	984	.	+	0	ID=annotation1686;Name=T7 TE;Parent=BBa_B0012
BBa_K077557	.	engineered_region	958	998	.	+	0	ID=annotation7020;Name=BBa_B0012;Parent=BBa_B0012
BBa_K077557	.	stop_codon	991	991	.	+	0	ID=annotation1687;Name=stop;Parent=BBa_B0012
BBa_K077557	.	promoter	1	54	.	+	0	ID=BBa_R0040;Name=BBa_R0040;Parent=BBa_S03119
BBa_K077557	.	promoter	43	48	.	+	0	ID=annotation1986787;Name=-10;Parent=BBa_R0040
BBa_K077557	.	non_covalent_binding_site	1	19	.	+	0	ID=annotation1986783;Name=TetR 1;Parent=BBa_R0040
BBa_K077557	.	promoter	20	25	.	+	0	ID=annotation1986785;Name=-35;Parent=BBa_R0040
BBa_K077557	.	non_covalent_binding_site	26	44	.	+	0	ID=annotation1986786;Name=TetR 2;Parent=BBa_R0040
BBa_K077557	.	engineered_region	1	54	.	+	0	ID=annotation1986784;Name=BBa_R0040;Parent=BBa_R0040
BBa_K077557	.	ribosome_entry_site	63	74	.	+	0	ID=BBa_B0034;Name=BBa_B0034;Parent=BBa_S03119
BBa_K077557	.	conserved_region	67	70	.	+	0	ID=annotation23325;Name=conserved;Parent=BBa_B0034
BBa_K077557	.	terminator	870	949	.	+	0	ID=BBa_B0010;Name=BBa_B0010;Parent=BBa_S03119
BBa_K077557	.	stem_loop	881	924	.	+	0	ID=annotation4184;Name=stem_loop;Parent=BBa_B0010
BBa_K077557	.	engineered_region	870	949	.	+	0	ID=annotation7018;Name=BBa_B0010;Parent=BBa_B0010
>BBa_K077557
tccctatcagtgatagagattgacatccctatcagtgatagagatactgagcactactagagaaagaggagaaatacta
gatgaaaaacataaatgccgacgacacatacagaataattaataaaattaaagcttgtagaagcaataatgatattaat
caatgcttatctgatatgactaaaatggtacattgtgaatattatttactcgcgatcatttatcctcattctatggtta
aatctgatatttcaatcctagataattaccctaaaaaatggaggcaatattatgatgacgctaatttaataaaatatga
tcctatagtagattattctaactccaatcattcaccaattaattggaatatatttgaaaacaatgctgtaaataaaaaa
tctccaaatgtaattaaagaagcgaaaacatcaggtcttatcactgggtttagtttccctattcatacggctaacaatg
gcttcggaatgcttagttttgcacattcagaaaaagacaactatatagatagtttatttttacatgcgtgtatgaacat
accattaattgttccttctctagttgataattatcgaaaaataaatatagcaaataataaatcaaacaacgatttaacc
aaaagagaaaaagaatgtttagcgtgggcatgcgaaggaaaaagctcttgggatatttcaaaaatattaggttgcagtg
agcgtactgtcactttccatttaaccaatgcgcaaatgaaactcaatacaacaaaccgctgccaaagtatttctaaagc
aattttaacaggagcaattgattgcccatactttaaaaattaataacactgatagtgctagtgtagatcactactagag
ccaggcatcaaataaaacgaaaggctcagtcgaaagactgggcctttcgttttatctgttgtttgtcggtgaacgctct
ctactagagtcacactggctcaccttcgggtgggcctttctgcgtttatatactagagtaagcacctgtaggatcgtac
aggtttacgcaagaaaatggtttgttatagtcgaataacaccgtgcgtgttgactattttacctctggcggtgatatac
tagagaaagaggagaaatactagatgactataatgataaaaaaatcggattttttggcaattccatcggaggagtataa
aggtattctaagtcttcgttatcaagtgtttaagcaaagacttgagtgggacttagttgtagaaaataaccttgaatca
gatgagtatgataactcaaatgcagaatatatttatgcttgtgatgatactgaaaatgtaagtggatgctggcgtttat
tacctacaacaggtgattatatgctgaaaagtgtttttcctgaattgcttggtcaacagagtgctcccaaagatcctaa
tatagtcgaattaagtcgttttgctgtaggtaaaaatagctcaaagataaataactctgctagtgaaattacaatgaaa
ctatttgaagctatatataaacacgctgttagtcaaggtattacagaatatgtaacagtaacatcaacagcaatagagc
gatttttaaagcgtattaaagttccttgtcatcgtattggagacaaagaaattcatgtattaggtgatactaaatcggt
tgtattgtctatgcctattaatgaacagtttaaaaaagcagtcttaaatgctgcaaacgacgaaaactacgctttagta
gcttaataactctgatagtgctagtgtagatctctactagagaaagaggagaaatactagatgcgtaaaggagaagaac
ttttcactggagttgtcccaattcttgttgaattagatggtgatgttaatgggcacaaattttctgtcagtggagaggg
tgaaggtgatgcaacatacggaaaacttacccttaaatttatttgcactactggaaaactacctgttccatggccaaca
cttgtcactactttcggttatggtgttcaatgctttgcgagatacccagatcatatgaaacagcatgactttttcaaga
gtgccatgcccgaaggttatgtacaggaaagaactatatttttcaaagatgacgggaactacaagacacgtgctgaagt
caagtttgaaggtgatacccttgttaatagaatcgagttaaaaggtattgattttaaagaagatggaaacattcttgga
cacaaattggaatacaactataactcacacaatgtatacatcatggcagacaaacaaaagaatggaatcaaagttaact
tcaaaattagacacaacattgaagatggaagcgttcaactagcagaccattatcaacaaaatactccaattggcgatgg
ccctgtccttttaccagacaaccattacctgtccacacaatctgccctttcgaaagatcccaacgaaaagagagaccac
atggtccttcttgagtttgtaacagctgctgggattacacatggcatggatgaactatacaaaaggcctgctgcaaacg
acgaaaactacgctttagtagcttaataatactagagccaggcatcaaataaaacgaaaggctcagtcgaaagactggg
cctttcgttttatctgttgtttgtcggtgaacgctctctactagagtcacactggctcaccttcgggtgggcctttctg
cgtttata