BBa_K078005 1 BBa_K078005 2，2???，3-Trihydroxybiphenyl 1,2-dioxygenase. The second step enzyme in dixon degradati 2008-10-14T11:00:00Z 2015-05-08T01:08:33Z from the dibenzofuran- and dibenzo-p-dioxin-degrading bacterium Sphingomonas sp. strain RW1. Type: genomic DNA 2，2???，3-Trihydroxybiphenyl 1,2-dioxygenase.The second step meta cleavage enzyme in dioxin degradation pathway. According to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used except Not1. The vector is the standard vector provided by biobrick08. We recommend double digestion of EcoRI+SpeI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h. false false _184_ 0 2670 9 It's complicated false We use it to construct the degradation pathway of dioxin. According to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used except Not1. The vector is the standard vector provided by biobrick08. We recommend double digestion of EcoRI+SpeI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h. false Sally.Wang (Jingyi Wang) annotation1983863 1 dbfB range1983863 1 1 885 BBa_K078005_sequence 1 atgtcagtaaaacaacttggctaccttatttttgaatgtagggctgatgttctggagcaaatggtagtcgtataccaagatatcatcggtgctgtggtggagcgtgatgaaggcggacgcgctcttgttcgccttgatggccgacctttccgtatcaggctcgatcctggccccgcaaaccgccttgcggcgatcggttggaatgtagaccctagtgatctggcggcgatagcggaacaggtggagaaggcatgttattcggtcgtaacggctgatgcggaactggccgcagatcgcgcagccgcccaagttcgacagttcgccgataacgatggctttacccatgaactgtatgtcgaatcgtcgtttcccaccgaccctgtgctcgagtctctattcgtctgtggtgaagaagcgaacggcatcttcggcttggggcacctcgtagtgatcgtggctgatcgggcgaagactcaatcttttttcactgatgttctgggcttcggactcagtgatcgggtaacttggcctgaagccgacatcttcttccttcactgcaaccagcgtcatcacaccgttgcactttcggcaccggcgctcggtcttaagccaggtatggttcatcatctgatgctagaagccaagagcaaagagcaggttgatcgcgcgttcgcagcggtcaagcgcttgggctatgatgtcctcatgacaattggccagcactccaatgataaggtctactccttctacatgatggcaccggctggttttgcagttgagttgggctttggtggccaggtgattggagatttggaaagttggcatgttggattctatgacgcaccgagcatttggggccacgagttgcagttgccggcgcattga igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z