BBa_K078102 1 BBa_K078102 dxnB 2008-10-23T11:00:00Z 2015-05-08T01:08:33Z an extradiol dioxygenase from the dibenzofuran- and dibenzo-p-dioxin-degrading bacterium Sphingomonas sp. strain RW1. Type: genomic DNA We use this part to construct the degradation pathway of dioxin. This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used . The vector is the standard vector,pSB1AC3, provided by biobrick08. We recommend double digestion of EcoRI+PstI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h. false false _184_ 0 2670 9 It's complicated false We use this part to construct the degradation pathway of dioxin. This is a standard parts. Acoording to the standard, we add EcoR1,Not1 and Xba1 upstream; and spe1, Not1, Pst1 and stop condon downstream. Anyone of the five sites could be used . The vector is the standard vector,pSB1AC3, provided by biobrick08. We recommend double digestion of EcoRI+PstI.Digest in NEBuffer EcoRI + BSA at 37??C for about 3h. false Sally.Wang (Jingyi Wang) BBa_K078102_sequence 1 gaattcgcggccgcttctagaatgacccagctacccagcaatttcatcgcaacttgtggataccgcacgcattacatcgagatgggtgagggcaagccactgatccttatccacggcggcggtgctggcgcggacgcccgcggaaactgggaaaaattgtgcatgccactactggcaaaggaccgcagggtaatagccctcgatatggtcggttttggctatagcgatgcacccgatccggaaggattcgattatggtctggagtgtcgcgttgatcagctaatcgcctttatcgaggccatgaatctggtcgaagcaaccggtcccgtggatatcatcggcaattccatgggcgggcgcaccacactggctaccgccatacgccgccccgacctcgtcggtgacatggtcctgatgggtagcgctggggtcgaccgctcgatgggcggcggggttgcgccgctgacagaatacgacttcacttacgccggcatggaacgtgtcgtcgcagcgttgaccaatgatcactacattccggacccagacatggtgcgatacaggctcgaactgtcacagcggcctgcctatgccaaggccctcagcgctaccttcaaggcgcttgtagcgcgatccggcctgtggatcgaggatgagaaaattgccgaggtccagcaccggacccttgtggttaacgggaaggctgacaaggttgtcccacccgaacacgcctataaattcctctctctcctaaccaattcaaccggcgtcatactcccccactgcgggcactgggcaatgatcgagcatccggttgcatttgctcatattacttcagcctttttcgctcggaaattctagtaataaagcctgcaggcggccgactagt igem2sbol 1 iGEM to SBOL conversion Conversion of the iGEM parts registry to SBOL2.1 Chris J. Myers James Alastair McLaughlin 2017-03-06T15:00:00.000Z