LOCUS       BBa_K079001             2075 bp    DNA     linear       22-Dec-2024
DEFINITION  LacI protein with GFP reporter protein
ACCESSION   BBa_K079001
VERSION     BBa_K079001.1  
FEATURES             Location/Qualifiers
     misc_feature    1090..1128
                     /label=LVA
     CDS             1..1128
                     /label=lacI-LVA
     misc_feature    1..1128
                     /label=BBa_C0012
     misc_feature    1129..1153
                     /label=Help:Barcodes
     CDS             1..1128
                     /label=BBa_C0012
     misc_feature    1166..1169
                     /label=conserved
     RBS             1162..1173
                     /label=BBa_B0034
     misc_feature    1180..1899
                     /label=BBa_E0040
     misc_feature    1894..1932
                     /label=LVA
     misc_feature    1933..1938
                     /label=Double Stop
     misc_feature    1180..1182
                     /label=Start
     misc_feature    1180..1938
                     /label=BBa_J04031
     stem_loop       1958..2001
                     /label=stem_loop
     misc_feature    1947..2026
                     /label=BBa_B0010
     terminator      1947..2026
                     /label=BBa_B0010
     stem_loop       2042..2061
                     /label=T7 TE
     misc_feature    2068..2068
                     /label=stop
     polyA_site      2062..2075
                     /label=polya
     misc_feature    2035..2075
                     /label=BBa_B0012
     terminator      2035..2075
                     /label=BBa_B0012
ORIGIN
        1 atggtgaatg tgaaaccagt aacgttatac gatgtcgcag agtatgccgg tgtctcttat
       61 cagaccgttt cccgcgtggt gaaccaggcc agccacgttt ctgcgaaaac gcgggaaaaa
      121 gtggaagcgg cgatggcgga gctgaattac attcccaacc gcgtggcaca acaactggcg
      181 ggcaaacagt cgttgctgat tggcgttgcc acctccagtc tggccctgca cgcgccgtcg
      241 caaattgtcg cggcgattaa atctcgcgcc gatcaactgg gtgccagcgt ggtggtgtcg
      301 atggtagaac gaagcggcgt cgaagcctgt aaagcggcgg tgcacaatct tctcgcgcaa
      361 cgcgtcagtg ggctgatcat taactatccg ctggatgacc aggatgccat tgctgtggaa
      421 gctgcctgca ctaatgttcc ggcgttattt cttgatgtct ctgaccagac acccatcaac
      481 agtattattt tctcccatga agacggtacg cgactgggcg tggagcatct ggtcgcattg
      541 ggtcaccagc aaatcgcgct gttagcgggc ccattaagtt ctgtctcggc gcgtctgcgt
      601 ctggctggct ggcataaata tctcactcgc aatcaaattc agccgatagc ggaacgggaa
      661 ggcgactgga gtgccatgtc cggttttcaa caaaccatgc aaatgctgaa tgagggcatc
      721 gttcccactg cgatgctggt tgccaacgat cagatggcgc tgggcgcaat gcgcgccatt
      781 accgagtccg ggctgcgcgt tggtgcggat atctcggtag tgggatacga cgataccgaa
      841 gacagctcat gttatatccc gccgttaacc accatcaaac aggattttcg cctgctgggg
      901 caaaccagcg tggaccgctt gctgcaactc tctcagggcc aggcggtgaa gggcaatcag
      961 ctgttgcccg tctcactggt gaaaagaaaa accaccctgg cgcccaatac gcaaaccgcc
     1021 tctccccgcg cgttggccga ttcattaatg cagctggcac gacaggtttc ccgactggaa
     1081 agcgggcagg ctgcaaacga cgaaaactac gctttagtag cttaataact ctgatagtgc
     1141 tagtgtagat ctctactaga gaaagaggag aaatactaga tgcgtaaagg agaagaactt
     1201 ttcactggag ttgtcccaat tcttgttgaa ttagatggtg atgttaatgg gcacaaattt
     1261 tctgtcagtg gagagggtga aggtgatgca acatacggaa aacttaccct taaatttatt
     1321 tgcactactg gaaaactacc tgttccatgg ccaacacttg tcactacttt cggttatggt
     1381 gttcaatgct ttgcgagata cccagatcat atgaaacagc atgacttttt caagagtgcc
     1441 atgcccgaag gttatgtaca ggaaagaact atatttttca aagatgacgg gaactacaag
     1501 acacgtgctg aagtcaagtt tgaaggtgat acccttgtta atagaatcga gttaaaaggt
     1561 attgatttta aagaagatgg aaacattctt ggacacaaat tggaatacaa ctataactca
     1621 cacaatgtat acatcatggc agacaaacaa aagaatggaa tcaaagttaa cttcaaaatt
     1681 agacacaaca ttgaagatgg aagcgttcaa ctagcagacc attatcaaca aaatactcca
     1741 attggcgatg gccctgtcct tttaccagac aaccattacc tgtccacaca atctgccctt
     1801 tcgaaagatc ccaacgaaaa gagagaccac atggtccttc ttgagtttgt aacagctgct
     1861 gggattacac atggcatgga tgaactatac aaaaggcctg ctgcaaacga cgaaaactac
     1921 gctttagtag cttaataata ctagagccag gcatcaaata aaacgaaagg ctcagtcgaa
     1981 agactgggcc tttcgtttta tctgttgttt gtcggtgaac gctctctact agagtcacac
     2041 tggctcacct tcgggtgggc ctttctgcgt ttata
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